CAJ | 학술논문

从某化工厂的活性污泥中分离纯化得到2株能以1,4-二氯苯为唯一碳源和能源生长的降解菌DEB-2和DEB-3,通过形态特征和生理生化实验初步鉴定为假单胞菌属(Pseudomonas)和芽孢杆菌属(Bacillus).根据细菌在有机相-水相两相体系中的细胞数量研究了DEB-2和DEB-3的表面疏水性以及在不同环境条件下的变化.同时对菌株DEB-2和DEB-3在液体培养条件下降解不同初始浓度的1,4-二氯苯的性能进行研究.结果表明,DEB-2和DEB-3的表面疏水性随培养时间、温度和pH值的变化而变化,并且细菌的细胞表面疏水性与其在水环境中对有机污染物的降解呈一定的相关性,疏水性大的菌株DEB-2对疏水性有机物的降解能力较疏水性小的菌株DEB-3要强.
종모화공엄적활성오니중분리순화득도2주능이1,4-이록분위유일탄원화능원생장적강해균DEB-2화DEB-3,통과형태특정화생리생화실험초보감정위가단포균속(Pseudomonas)화아포간균속(Bacillus).근거세균재유궤상-수상량상체계중적세포수량연구료DEB-2화DEB-3적표면소수성이급재불동배경조건하적변화.동시대균주DEB-2화DEB-3재액체배양조건하강해불동초시농도적1,4-이록분적성능진행연구.결과표명,DEB-2화DEB-3적표면소수성수배양시간、온도화pH치적변화이변화,병차세균적세포표면소수성여기재수배경중대유궤오염물적강해정일정적상관성,소수성대적균주DEB-2대소수성유궤물적강해능력교소수성소적균주DEB-3요강.
Two strains named DEB-2 and DEB-3 that degraded dichlorobenzene as sole carbon and energy source were isolated from activated sludge of a chemical plant. They were identified as Pseudomonas and Bacillus according to the morphology and biochemical properties. Surface hydrophobicity of the two strains and their changes under different environmental conditions were studied based on cell amount of bacteria in organic water two- phase system. The degradation of different initial concentrations of dichlorobenzene by DEB-2 and DEB-3 were carried out in solution. Results showed that hydrophobicity changed with the change of cultivation time, temperature and pH value. The hydrophobicity of the two strains were related definitely with the degradation of organic pollutant in water environment, with the degradation capability of hydrophobic organics by the strain DEB-2 of high hydrophobicity stronger than that by the swain DEB-3 of low hydrophobicity.