CAJ | 학술논문

背景:目前常采用在骨缺损处充填自体骨、异体骨、人工合成骨替代品等方法进行骨缺损修复,但临床效果均不理想.研究表明脂肪间充质干细胞具有多种分化能力及很强的增殖潜力,可将其诱导分化成骨.目的:分析脂肪间充质干细胞在骨组织工程中的应用情况,以明确脂肪间充质干细胞是否可以作为骨组织工程中的种子细胞.方法:应用计算机检索Medline数据库(1999-01/2008-12),以adipose tissue-derived mesenchymal stem cells,adipose mesenchymal stem cells,adipose stem cell;osteogenic induction,osteogenic inducement,bone induction,osteoblastic induced;chondroblast induction,cartilage induction;bone tissue engineering,tissue engineering bone,tissue engineering of bone为检索词;应用计算机检索清华同方数据库(2003-01/2008-12),以脂肪间充质干细胞、成骨诱导、成软骨诱导、骨组织工程为检索词.结果与结论:共收集361篇关于脂肪间充质干细胞与骨组织工程相关的文献,中文246篇,英文115篇.排除发表时间较早、重复及类似研究,纳入29篇符合标准的文献.脂肪间充质干细胞体外扩增和自我更新能力很强,传代培养易于获得大量有分化能力的细胞,是真正意义上的具有多向分化潜能的干细胞,脂肪间充质干细胞在一定的培养条件下,可定向分化为成骨细胞、软骨细胞及骨细胞和肌肉细胞,配合合适的支架后可以应用到骨组织工程中,可以作为骨组织工程中的种子细胞.
배경:목전상채용재골결손처충전자체골、이체골、인공합성골체대품등방법진행골결손수복,단림상효과균불이상.연구표명지방간충질간세포구유다충분화능력급흔강적증식잠력,가장기유도분화성골.목적:분석지방간충질간세포재골조직공정중적응용정황,이명학지방간충질간세포시부가이작위골조직공정중적충자세포.방법:응용계산궤검색Medline수거고(1999-01/2008-12),이adipose tissue-derived mesenchymal stem cells,adipose mesenchymal stem cells,adipose stem cell;osteogenic induction,osteogenic inducement,bone induction,osteoblastic induced;chondroblast induction,cartilage induction;bone tissue engineering,tissue engineering bone,tissue engineering of bone위검색사;응용계산궤검색청화동방수거고(2003-01/2008-12),이지방간충질간세포、성골유도、성연골유도、골조직공정위검색사.결과여결론:공수집361편관우지방간충질간세포여골조직공정상관적문헌,중문246편,영문115편.배제발표시간교조、중복급유사연구,납입29편부합표준적문헌.지방간충질간세포체외확증화자아경신능력흔강,전대배양역우획득대량유분화능력적세포,시진정의의상적구유다향분화잠능적간세포,지방간충질간세포재일정적배양조건하,가정향분화위성골세포、연골세포급골세포화기육세포,배합합괄적지가후가이응용도골조직공정중,가이작위골조직공정중적충자세포.
BACKGROUND: At present, bone defects usually repaired by autologous bone, allogenic bone, synthetic bone substitutes and other methods, which received poor clinical results. Preliminary studies have shown that adipose-derived mesenchymal stem cells (ADSCs) possess strong proliferation ability and differentiation potential, and can be induced differentiate into bone. OBJECTIVE: To analyze the application of ADSCs in bone tissue engineering, and to identify whether ADSCs can be used as seed cells in bone tissue engineering. METHODS: The databases of PubMed (1999-01/2008-12) and Tongfang (2003-01/2008-12) was retrieved using key words of "adipose tissue-derived mesenchymal stem cells, adipose mesenchymal stem cells, adipose stem cell; osteogenic induction, osteogenic inducement, bone induction, osteoblastic induced; chondroblast induction, cartilage induction; bone tissue engineering, tissue engineering bone, tissue engineering of bone". RESULTS AND CONCLUSION: A total of 361 literatures were collected, including 246 in Chinese and 115 in English. Totally 29 literatures were accordant with the study criteria. ADSCs is a truly multi-directional differentiation potential cells, which possess strong amplification and self-renewal potential, and can be directional differentiated into osteoblasts, cartilage cells, bone cells and muscle cells. It can be used as seed cells in bone tissue engineering when matching appropriate stents.