CAJ | 학술논문

WRKY是植物特有的转录因子家族,主要参与植物的各种防卫反应,调节植物生长发育等.本文运用多元统计分析和相关分析等方法,对拟南芥和水稻WRKY基因的密码子用法进行了分析,发现两个物种WRKY基因的碱基组成不同,水稻基因在密码子第1、2和3位的GC含量均高于拟南芥,第3位差异最大.但两个物种的WRKY基因存在共同的进化趋势,即基因的GC3s逐步增大.对应分析结果显示,拟南芥WRKY基因的密码子使用偏好性受碱基组成等多种因素共同影响,水稻主要受碱基组成和基因表达水平两个因素的影响.最后确定了拟南芥和水稻WRKY基因家族的最优密码子,分别为11个和27个.研究结果为深入开展WRKY基因的进化、表达调控和提高该基因家族新成员预测的准确性等具有重要指导意义.
WRKY시식물특유적전록인자가족,주요삼여식물적각충방위반응,조절식물생장발육등.본문운용다원통계분석화상관분석등방법,대의남개화수도WRKY기인적밀마자용법진행료분석,발현량개물충WRKY기인적감기조성불동,수도기인재밀마자제1、2화3위적GC함량균고우의남개,제3위차이최대.단량개물충적WRKY기인존재공동적진화추세,즉기인적GC3s축보증대.대응분석결과현시,의남개WRKY기인적밀마자사용편호성수감기조성등다충인소공동영향,수도주요수감기조성화기인표체수평량개인소적영향.최후학정료의남개화수도WRKY기인가족적최우밀마자,분별위11개화27개.연구결과위심입개전WRKY기인적진화、표체조공화제고해기인가족신성원예측적준학성등구유중요지도의의.
WRKY, a family of transcription factors peculiar to plant, participates in various kinds of defense responses and regulates growth and development in plant.In this study, the codon usage of WRKY genes in Arabidopsis and rice was analyzed using methods of multivariate statistical analysis and correlation analysis.The results showed that nucleotide composition of WRKY genes in the two species were obviously different.The G+C contents at the first, second and third codon positions of rice were higher than those of Arabidopsis, and the difference of G+C content at the third codon position was most significant.As WRKY gene family evolves, they prefer the codons with C or G at the synonymous position.Through the correspondence analysis, it was found that the codon usage of WRKY genes in Arabidopsis was affected by many factors, such as the nucleotide composition.The nucleotide composition and gene expression level were the main factors in shaping the codon usage in rice.Additionally, 11 codons in Arabidopsis and 27 codons in rice were defined as 'optimal codons'.The results provide important guidance for gene evolution, regulation of gene expression and gene prediction of WRKY family.