CAJ | 학술논문

背景:有报道显示美国处于高风险的骨质疏松妇女中磷水平较高,这是否意味着降低血磷水平的物质将会为防治骨质疏松提供新的有效手段?目的:观察猪骨蛋白对骨质疏松大鼠骨密度和血清中钙及磷水平的影响.方法:以肌肉注射地塞米松建立Wistar大鼠骨质疏松模型.造模后以数字表法随机分为生理盐水组、接骨七哩片组、50,100,200 mg/kg猪骨蛋白组,不作任何处置大鼠作为正常对照.治疗12周后,分离血清并用生物化学方法测定血清磷和血钙水平,同时收集大鼠胫骨制作成骨切片,以QDR-4000双能X射线吸收仪测定各组大鼠胫骨吸光度值;苏木精-伊红染色观察胫骨骨髓腔变化.结果与结论:各组之间血清钙浓度比较差异无显著性意义(P＞0.05).与生理盐水组比较,50,100,200 mg/kg猪骨蛋白组大鼠血清磷浓度下降(P＜0.05).50,100,200 mg/kg猪骨蛋白组、接骨七哩片组骨密度值高于生理盐水组(P＜0.05).正常对照组大鼠胫骨的骨髓腔是小的,生理盐水组大鼠胫骨的骨髓腔特别大,50,100,200 mg/kg猪骨蛋白组、接骨七哩片组大鼠胫骨骨髓腔比生理盐水组大鼠胫骨骨髓腔小.结果提示猪骨蛋白不改变骨质疏松大鼠血清钙的水平,但它能降低骨质疏松大鼠血清磷的浓度,增加骨密度.不过,在该实验浓度范围内,没有显示剂量效应关系;猪骨蛋白也能缩小骨质疏松大鼠胫骨骨髓腔.
배경:유보도현시미국처우고풍험적골질소송부녀중린수평교고,저시부의미착강저혈린수평적물질장회위방치골질소송제공신적유효수단?목적:관찰저골단백대골질소송대서골밀도화혈청중개급린수평적영향.방법:이기육주사지새미송건립Wistar대서골질소송모형.조모후이수자표법수궤분위생리염수조、접골칠리편조、50,100,200 mg/kg저골단백조,불작임하처치대서작위정상대조.치료12주후,분리혈청병용생물화학방법측정혈청린화혈개수평,동시수집대서경골제작성골절편,이QDR-4000쌍능X사선흡수의측정각조대서경골흡광도치;소목정-이홍염색관찰경골골수강변화.결과여결론:각조지간혈청개농도비교차이무현저성의의(P＞0.05).여생리염수조비교,50,100,200 mg/kg저골단백조대서혈청린농도하강(P＜0.05).50,100,200 mg/kg저골단백조、접골칠리편조골밀도치고우생리염수조(P＜0.05).정상대조조대서경골적골수강시소적,생리염수조대서경골적골수강특별대,50,100,200 mg/kg저골단백조、접골칠리편조대서경골골수강비생리염수조대서경골골수강소.결과제시저골단백불개변골질소송대서혈청개적수평,단타능강저골질소송대서혈청린적농도,증가골밀도.불과,재해실험농도범위내,몰유현시제량효응관계;저골단백야능축소골질소송대서경골골수강.
BACKGROUND: Several studies have demonstrated that many American women who are at high risk of developing osteoporosis have higher levels of serum phosphorus. This indicates that some substances which can lower the serum level of phosphorus will supply a new and effective method to prevent and treat osteoporosis.OBJECTIVE: To observe the influences of porcine bone protein on bone mineral density (BMD) and serum levels of calcium and phosphorus in a rat model of osteoporosis.METHODS: Wistar rat models of osteoporosis were established by intramuscular injection of dexamethasone. Rat models were randomly divided into physiological saline, Jiegu Qili tablet, 50, 100, 200 mg/kg porcine bone protein groups. Rats that did not receive any treatments served as normal controls. After 12 weeks of treatment, serum was collected and serum levels of phosphorus and calcium were determined by biochemistry method. At the same time, tibia sections were made to determine tibial DMD by QDR-400 dual energy X-ray absorptiometry and to observe tibia marrow cavity by hematoxylin-eosin staining.RESULTS AND CONCLUSION: There was no significant difference in serum level of calcium among groups (P＞0.05).Compared with the physiological saline group, serum level of phosphorus in the 50, 100, 200 mg/kg porcine bone protein groups was significantly decreased (P ＜ 0.05). BMD was significantly higher in the 50, 100, 200 mg/kg porcine bone protein, Jiegu Qili tablet groups than in the physiological saline group (P ＜ 0.05). The tibia marrow cavity was smallest in the normal control group and largest in the physiological saline group. The tibia marrow cavity was larger in the 50, 100, 200 mg/kg porcine bone protein,Jiegu Qili tablet groups than in the physiological saline group. These results indicate that porcine bone protein cannot change the serum level of calcium, but it lowers serum level of phosphorus, and increases BMD, in a rat model of osteoporosis. However, the dose-dependent effect of porcine bone protein was not observed within the present experimental dosage. In addition, porcine bone protein can also reduce the marrow cavity of the tibia of rats with osteoporosis.