CAJ | 학술논문

目的观察巨噬细胞肿瘤疫苗对细胞毒性T细胞(CTL)反应及Th1/Th2型细胞因子分泌的调节作用.方法分别采用MTT法和比色法检测肿瘤细胞杀伤率和清液上乳酸脱氢酶(LDH)含量,采用Western印记法与ELISA法检测脾细胞内及分泌入培养上清的IL-2、IL-4、IL-10及IFN-У等细胞因子.结果巨噬细胞肿瘤疫苗接种组的淋巴细胞肿瘤细胞杀伤率与培养上清液LDH水平分别为(36.70±21.02)%和(0.29±0.15)U/ml,明显高于热灭活肿瘤细胞接种组、石蜡诱导的巨噬细胞接种组(P值均<0.05).肿瘤细胞冻融物刺激72 h后,各组免疫小鼠的脾细胞内均可检测到IL-2、IL-4、IL-10及IFN-У等细胞因子;同时巨噬细胞肿瘤疫苗接种组脾细胞培养上清中,IL-2、IL-4、IL-10及IFN-У含量分别为(7.97±3.15)ps/ml、(0.44±0.11)pg/ml、(1.83±0.85)pg/ml和(9.16±4.64)pg/ml,IL-2、IFN-У的水平均高于热灭活肿瘤细胞接种组和石蜡诱导的巨噬细胞接种组(P值均<0.05).结论巨噬细胞肿瘤疫苗可诱导机体产生特异性抗肿瘤反应,能够促进Th1型细胞因子IL-2、IFN-У的分泌.
목적 관찰거서세포종류역묘대세포독성T세포(CTL)반응급Th1/Th2형세포인자분비적조절작용.방법 분별채용MTT법화비색법검측종류세포살상솔화청액상유산탈경매(LDH)함량,채용Western인기법여ELISA법검측비세포내급분비입배양상청적IL-2、IL-4、IL-10급IFN-У등세포인자.결과 거서세포종류역묘접충조적림파세포종류세포살상솔여배양상청액LDH수평분별위(36.70±21.02)%화(0.29±0.15)U/ml,명현고우열멸활종류세포접충조、석사유도적거서세포접충조(P치균<0.05).종류세포동융물자격72 h후,각조면역소서적비세포내균가검측도IL-2、IL-4、IL-10급IFN-У등세포인자;동시거서세포종류역묘접충조비세포배양상청중,IL-2、IL-4、IL-10급IFN-У함량분별위(7.97±3.15)ps/ml、(0.44±0.11)pg/ml、(1.83±0.85)pg/ml화(9.16±4.64)pg/ml,IL-2、IFN-У적수평균고우열멸활종류세포접충조화석사유도적거서세포접충조(P치균<0.05).결론 거서세포종류역묘가유도궤체산생특이성항종류반응,능구촉진Th1형세포인자IL-2、IFN-У적분비.
Objective To investgate the effect of macrophage tumor vaccine on the regulation of CTL response and Th1/Th2 type cytokines production. Methods The tumor cell injury rate and the level of LDH in culture pressed in spleen cells and released into culture supernatant were detected with Western bolting and ELISA, re-spectively. Results The tumor injury rate and the level of LDH in culture supernatant of macrophage tumor vaccine immunized group were (36. 70 +21.02)% and (0. 29 +0. 15) U/ml, respectively. Both are signifi-cantly higher than that of the group immunized with heat inactivated tumor cells and the group immunized with liquid paraffin induced macrophages (P < 0. 05). After stimulating 72 hours with the freeze thawing tumor cells, IL-10 and IFN-T in culture supernatant of macrophage tumor vaccine immunized group were (7. 97 ±3. 15) pg/ml, (0. 44 ±0. 11) pg/ml, (1.83±0. 85) pg/ml and (9. 16±4. 64) pg/ml, respectively. All of them obvi-ously higher than that of the group of immunized with heat inactivated tumor cells and the group immunized with liquid paraffin induced macrophages(P <0. 05). Condusion Macrophage tumor vaccine could elicit specific