中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2012年
1期
10-12
,共3页
慢性不可预知温和应激模型%神经干细胞%海马
慢性不可預知溫和應激模型%神經榦細胞%海馬
만성불가예지온화응격모형%신경간세포%해마
Chronic unpredictable mild stress%Neural stem cell%Hippocampus
目的 探讨慢性应激抑郁状态对大鼠海马神经干细胞的影响.方法 应用慢性不可预知温和应激和孤养法建立抑郁模型,分为对照组、CUMS 14 d组、CUMS 28 d组,采用免疫组化、免疫荧光,测定大鼠海马神经干细胞的增殖、存活和分化的改变.结果 海马神经干细胞增殖:与对照组( 2919.50±188.80)比较,14d CUMS组(2254.17±164.41)和28 d CUMS组(1900.33±104.10)海马齿状回BrdU阳性细胞数均减少,差异有统计学意义(P＜0.01).海马神经干细胞存活:与对照组( 2404.50±148.77)相比,CUMS 28 d组(1845.33±126.88) BrdU阳性细胞数明显减少,差异有统计学意义(P＜0.01).海马神经干细胞分化:与对照组[ NeuN/BrdU(71.63±10.21),GFAP/BrdU( 14.34±2.03)]比较,CUMS 28d组[NeuN/BrdU(69.11±11.30),GFAP/BrdU( 17.27±2.93)]细胞分化无明显变化(P＞0.05).结论 慢性应激抑郁模型大鼠海马齿状回神经干细胞的增殖和存活受到抑制,但干细胞的分化无明显改变.
目的 探討慢性應激抑鬱狀態對大鼠海馬神經榦細胞的影響.方法 應用慢性不可預知溫和應激和孤養法建立抑鬱模型,分為對照組、CUMS 14 d組、CUMS 28 d組,採用免疫組化、免疫熒光,測定大鼠海馬神經榦細胞的增殖、存活和分化的改變.結果 海馬神經榦細胞增殖:與對照組( 2919.50±188.80)比較,14d CUMS組(2254.17±164.41)和28 d CUMS組(1900.33±104.10)海馬齒狀迴BrdU暘性細胞數均減少,差異有統計學意義(P＜0.01).海馬神經榦細胞存活:與對照組( 2404.50±148.77)相比,CUMS 28 d組(1845.33±126.88) BrdU暘性細胞數明顯減少,差異有統計學意義(P＜0.01).海馬神經榦細胞分化:與對照組[ NeuN/BrdU(71.63±10.21),GFAP/BrdU( 14.34±2.03)]比較,CUMS 28d組[NeuN/BrdU(69.11±11.30),GFAP/BrdU( 17.27±2.93)]細胞分化無明顯變化(P＞0.05).結論 慢性應激抑鬱模型大鼠海馬齒狀迴神經榦細胞的增殖和存活受到抑製,但榦細胞的分化無明顯改變.
목적 탐토만성응격억욱상태대대서해마신경간세포적영향.방법 응용만성불가예지온화응격화고양법건립억욱모형,분위대조조、CUMS 14 d조、CUMS 28 d조,채용면역조화、면역형광,측정대서해마신경간세포적증식、존활화분화적개변.결과 해마신경간세포증식:여대조조( 2919.50±188.80)비교,14d CUMS조(2254.17±164.41)화28 d CUMS조(1900.33±104.10)해마치상회BrdU양성세포수균감소,차이유통계학의의(P＜0.01).해마신경간세포존활:여대조조( 2404.50±148.77)상비,CUMS 28 d조(1845.33±126.88) BrdU양성세포수명현감소,차이유통계학의의(P＜0.01).해마신경간세포분화:여대조조[ NeuN/BrdU(71.63±10.21),GFAP/BrdU( 14.34±2.03)]비교,CUMS 28d조[NeuN/BrdU(69.11±11.30),GFAP/BrdU( 17.27±2.93)]세포분화무명현변화(P＞0.05).결론 만성응격억욱모형대서해마치상회신경간세포적증식화존활수도억제,단간세포적분화무명현개변.
Objective To investigate the effect of chronic stress on hippocampal neurogenesis.Methods Chronic unpredictable mild stress(CUMS) was used as animal model to provoke a decrease of neurogenesis in hippocampus.Simultaneously,hippocampal neurogenesis was monitored by assessing cell proliferation,survival,and differentiation.All rats were divided into control group,14 d CUMS group,28 d CUMS group.Results The cell proliferation in 14d CUMS group(2254.17 ± 164.41 ) or 28d CUMS( 1900.33 ± 104.10) group were decreased contrast to the control (2919.50 ± 188.80) (P＜0.01).The cell survival in 28 d CUMS group ( 1845.33± 126.88 ) were decreased contrast to the control (2404.50 ± 148.77 ) (P ＜ 0.01 ).The cell differentiation had no difference between control group and CUMS group( NeuN/BrdU(71.63 ± 10.21 ) vs (69.11 ± 11.30),GFAP/BrdU ( 14.34 ± 2.03 ) vs ( 17.27 ± 2.93 ) ) (P ＞ 0.05 ).Conclusion These results suggest that chronic stress decreases cell proliferation and survival,but does not affect the phenotype of newborn cells in the hippocampus.
