CAJ | 학술논문

目的探讨不同浓度七叶皂苷钠对兔耳缘静脉组织病理学的影响.方法新西兰白兔32只,完全随机分为4组:低剂量组、中剂量组、高剂量组和对照组,每组8只.低、中、高剂量组分别在15 min内耳缘静脉滴注1、2、4 mg七叶皂苷钠(均用生理盐水配制成10 ml的溶液),对照组耳缘予静脉滴注同体积生理盐水,连续给药3 d.给药过程中观察注射部位的不良反应,第3天给药结束30 min后处死各组兔.用光学显微镜观察耳缘静脉穿刺周围的组织病理学改变.结果各实验组随药物浓度增加,静脉穿刺周围沿静脉走向呈现程度递增的静脉炎特征,而对照组无此类变化.中剂量组近心端血管内皮细胞缺失、炎症细胞浸润、组织水肿评分分别为[1.0(1.5)、1.5(1.0)、1.0(0.5)分],远心端血管内皮细胞缺失、炎症细胞浸润评分分别为[2.0(2.0)、1.0(2.0)分];高剂量组近、远心端血管内皮细胞缺失、炎症细胞浸润、组织水肿评分分别为[2.0(1.0)、2.0(0.0)、2.0(1.0)分,3.0(1.0)、2.0(2.0)、2.0(2.0)分],上述各项与对照组[0.0(0.0)、0.0(0.0)、0.0(0:5)分,0.0(0.0)、0.0(0.0)、0.5(1.0)分]比较差异均有统计学意义(P<0.05),并且随着作用浓度的增加,低、中、高剂量组病理损伤程度逐步加重.高剂量组的病理损伤最重,具备了静脉炎的基本病理特征.符号秩检验结果显示,高剂量组兔耳近心端与远心端病理损伤程度差异无统计学意义(P>0.05).结论七叶皂苷钠导致兔耳输液性静脉炎的毒性与其输注剂量有关,剂量越高,静脉炎的严重程度越高.
목적 탐토불동농도칠협조감납대토이연정맥조직병이학적영향.방법 신서란백토32지,완전수궤분위4조:저제량조、중제량조、고제량조화대조조,매조8지.저、중、고제량조분별재15 min내이연정맥적주1、2、4 mg칠협조감납(균용생리염수배제성10 ml적용액),대조조이연여정맥적주동체적생리염수,련속급약3 d.급약과정중관찰주사부위적불량반응,제3천급약결속30 min후처사각조토.용광학현미경관찰이연정맥천자주위적조직병이학개변.결과 각실험조수약물농도증가,정맥천자주위연정맥주향정현정도체증적정맥염특정,이대조조무차류변화.중제량조근심단혈관내피세포결실、염증세포침윤、조직수종평분분별위[1.0(1.5)、1.5(1.0)、1.0(0.5)분],원심단혈관내피세포결실、염증세포침윤평분분별위[2.0(2.0)、1.0(2.0)분];고제량조근、원심단혈관내피세포결실、염증세포침윤、조직수종평분분별위[2.0(1.0)、2.0(0.0)、2.0(1.0)분,3.0(1.0)、2.0(2.0)、2.0(2.0)분],상술각항여대조조[0.0(0.0)、0.0(0.0)、0.0(0:5)분,0.0(0.0)、0.0(0.0)、0.5(1.0)분]비교차이균유통계학의의(P<0.05),병차수착작용농도적증가,저、중、고제량조병리손상정도축보가중.고제량조적병리손상최중,구비료정맥염적기본병리특정.부호질검험결과현시,고제량조토이근심단여원심단병리손상정도차이무통계학의의(P>0.05).결론 칠협조감납도치토이수액성정맥염적독성여기수주제량유관,제량월고,정맥염적엄중정도월고.
Objective To investigate the histopathologic changes in the rabbits' ear veins induced by different concentrations of sodium aescinate(SA). Methods Thirty-two rabbits were randomly divided into four groups (8 in each group) : the low-dose SA group, the moderate-dose SA group, the high-dose SA group and the control group. The first 3 groups were respectively infused with 1 mg/10 ml, 2 mg/10 ml and 4 mg/10 ml solutions of SA into one ear vein within 15 min 3 days. The control group was administrated with the same volume of normal saline. The ears of rabbit were observed every day for adverse reactions. After the last infusion, the rabbits were killed by an overdose of sodium pentobarbital. The histopathologic changes in the rabbits' ear veins were observed under light microscope. Results Compared with the control group, infusion of sodium aescine of different concentrations caused marked pathological damage on the part around the ear vein. The median (interquartile range) of the scores of the loss of venous endothelial cells, inflammatory cell infiltration and edema of the proximal regions in the moderate-dose SA group were 1.0(1.5),1.5(1.0),1.0(0.5) scores, the loss of venous endothelial cells and inflammatory cell infiltration of the distal regions in the moderate-dose SA group were 2.0(2.0) ,1.0(2.0) scores, the loss of venous endothelial cells, inflammatory cell infiltration and edema of the proximal and distal regions in the high-dose SA group were 2.0(1.0) ,2.0(0. 0) ,2. 0(1. 0) scores and 3.0(1.0) ,2. 0(2. 0) ,2. 0(2. 0) scores. Compared with the control group,the above indicators were statistically significant differences(P<0.05). The high-dose SA group(4 mg/10 ml) displayed the most severe phlebitis symptoms. There were no statistically significant differences in pathological damages between the proximal and distal regions in the high-dose SA group (P > 0. 05 ). Conclusions The phlebitic effect of SA on rabbits is related to dosage. Specifically, higher dosage of the drug can cause more severe phlebitis.