中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2011年
6期
566-572
,共7页
刘元刚%程欲%张晨%刘树滔%饶平凡
劉元剛%程欲%張晨%劉樹滔%饒平凡
류원강%정욕%장신%류수도%요평범
伤口愈合%抗氧化剂%氧化性应激%活性氧
傷口愈閤%抗氧化劑%氧化性應激%活性氧
상구유합%항양화제%양화성응격%활성양
Wound healing%Antioxidants%Oxidative stress%Reactive oxygen species
目的 探讨氧化应激在机械性创伤愈合发展中的地位、作用和抗氧化剂PTD-SOD对创伤愈合的影响.方法 制备机械性创伤小鼠模型和不同浓度的PTD-SOD(1 000 U、3 000 U、6 000 U、10 000 U)和SOD(3 000 U、6 000 U)溶液,分别用上述溶液进行治疗,同时设立模型对照组、等渗盐水对照组和复方碘液对照组,各组均连续治疗13天.观察各组创伤愈合情况,记录创伤收缩率和愈合天数;于创伤后第14天取各组小鼠创伤愈合部位皮肤,一部分制成10%组织匀浆液用于检测抗氧化酶(SOD、CAT、GSH-Px)活性及丙二醛(MDA)、羟脯氨酸(Hyp)含量,一部分制成病理组织切片用于皮肤组织学观察.结果 (1)与模型对照组、等渗盐水对照组及复方碘液对照组相比,同时相点PTD-SOD各组(10 000 U组除外)和SOD各组的抗氧化酶活性和Hyp含量显著升高(P<0.05或0.01),MDA含量显著降低(P<0.05或0.01),创伤收缩率提高(P<0.05或0.01),创伤愈合时间缩短(P<0.05或0.01).(2)在同等剂量下,从促创伤愈合时间、抗氧化酶活性、MDA含量、Hyp含量等方面比较,伤后同时相点PTD-SOD组明显优于SOD组(P<0.05或0.01).(3)10 000 U PTD-SOD组创伤愈合效果明显不如其他剂量PTD-SOD组(P<0.05或0.01).结论 在创伤愈合的早期特别是炎症期,应考虑氧化应激的存在,抗氧化治疗是一个治疗策略,适时、适当的抗氧化治疗,可以抑制氧化应激态,缩短创面愈合时间,提高创面愈合质量.适当剂量的PTD-SOD在皮肤创伤治疗中具有很好的抗氧化应激损伤、抑制炎症和促进愈合的效果.
目的 探討氧化應激在機械性創傷愈閤髮展中的地位、作用和抗氧化劑PTD-SOD對創傷愈閤的影響.方法 製備機械性創傷小鼠模型和不同濃度的PTD-SOD(1 000 U、3 000 U、6 000 U、10 000 U)和SOD(3 000 U、6 000 U)溶液,分彆用上述溶液進行治療,同時設立模型對照組、等滲鹽水對照組和複方碘液對照組,各組均連續治療13天.觀察各組創傷愈閤情況,記錄創傷收縮率和愈閤天數;于創傷後第14天取各組小鼠創傷愈閤部位皮膚,一部分製成10%組織勻漿液用于檢測抗氧化酶(SOD、CAT、GSH-Px)活性及丙二醛(MDA)、羥脯氨痠(Hyp)含量,一部分製成病理組織切片用于皮膚組織學觀察.結果 (1)與模型對照組、等滲鹽水對照組及複方碘液對照組相比,同時相點PTD-SOD各組(10 000 U組除外)和SOD各組的抗氧化酶活性和Hyp含量顯著升高(P<0.05或0.01),MDA含量顯著降低(P<0.05或0.01),創傷收縮率提高(P<0.05或0.01),創傷愈閤時間縮短(P<0.05或0.01).(2)在同等劑量下,從促創傷愈閤時間、抗氧化酶活性、MDA含量、Hyp含量等方麵比較,傷後同時相點PTD-SOD組明顯優于SOD組(P<0.05或0.01).(3)10 000 U PTD-SOD組創傷愈閤效果明顯不如其他劑量PTD-SOD組(P<0.05或0.01).結論 在創傷愈閤的早期特彆是炎癥期,應攷慮氧化應激的存在,抗氧化治療是一箇治療策略,適時、適噹的抗氧化治療,可以抑製氧化應激態,縮短創麵愈閤時間,提高創麵愈閤質量.適噹劑量的PTD-SOD在皮膚創傷治療中具有很好的抗氧化應激損傷、抑製炎癥和促進愈閤的效果.
목적 탐토양화응격재궤계성창상유합발전중적지위、작용화항양화제PTD-SOD대창상유합적영향.방법 제비궤계성창상소서모형화불동농도적PTD-SOD(1 000 U、3 000 U、6 000 U、10 000 U)화SOD(3 000 U、6 000 U)용액,분별용상술용액진행치료,동시설립모형대조조、등삼염수대조조화복방전액대조조,각조균련속치료13천.관찰각조창상유합정황,기록창상수축솔화유합천수;우창상후제14천취각조소서창상유합부위피부,일부분제성10%조직균장액용우검측항양화매(SOD、CAT、GSH-Px)활성급병이철(MDA)、간포안산(Hyp)함량,일부분제성병리조직절편용우피부조직학관찰.결과 (1)여모형대조조、등삼염수대조조급복방전액대조조상비,동시상점PTD-SOD각조(10 000 U조제외)화SOD각조적항양화매활성화Hyp함량현저승고(P<0.05혹0.01),MDA함량현저강저(P<0.05혹0.01),창상수축솔제고(P<0.05혹0.01),창상유합시간축단(P<0.05혹0.01).(2)재동등제량하,종촉창상유합시간、항양화매활성、MDA함량、Hyp함량등방면비교,상후동시상점PTD-SOD조명현우우SOD조(P<0.05혹0.01).(3)10 000 U PTD-SOD조창상유합효과명현불여기타제량PTD-SOD조(P<0.05혹0.01).결론 재창상유합적조기특별시염증기,응고필양화응격적존재,항양화치료시일개치료책략,괄시、괄당적항양화치료,가이억제양화응격태,축단창면유합시간,제고창면유합질량.괄당제량적PTD-SOD재피부창상치료중구유흔호적항양화응격손상、억제염증화촉진유합적효과.
Objective To study the role of the oxidative stress in the development of wound healing and observe the effect of the antioxidant PTD-SOD on damage and inflammation reaction after mechanical wound. Methods In this experiment,acute wound healing model by removal the whole layer dorsal skin of the mice was prepared,SOD(3 000 U and 6 000 U)and the fusion protein PTD-SOD with different concentrations(1 000 U,3 000 U,6 000 U and 10 000 U)were used to deal with the wounds continuously for 13 days.The mice were divided into different concentration SOD treatment group and PTD-SOD treatment group,model control group,physiological saline treatment group and compound iodine solution control group.The wound healing situation and healing percentage of the fight and left skin wounds of each mouse in every group was recorded every day.At day 14 after wound,the wound healing skin of each group was removed and some were used to make 10%tissues homogenate for detecting the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)and contents of malondialdehyde(MDA)and hydroxyproline(Hyp);in the meantime,the other removed skin were fixed in 10% formalin for observing the histopathological changes of the tissues. Results Compared with the model control group,the physiological saline treatment group and the compound iodine solution control group,the skin wound healing percentage was significantly(P<0.05 or P<0.01)improved,with increase of the activities of SOD,CAT,GSH-Px and contents of Hyp (P<0.05 or P<0.01)and decrease of MDA(P<0.05 or P<0.01) in the SOD groups or PTD-SOD groups (except for 10 000 U PTD-SOD group).When compared with the physiological saline treatment group or the compound iodine solution treatment group,the effect was similar to the model control group.In comparison to the SOD groups,under the same dosage and environment condition,the PTD-SOD groups were much better than SOD groups with regard to promoting skin wound healing percentage,increasing activities of antioxidases and contents of Hyp,decreasing contents of MDA.Among the PTD-SOD groups,the effect of high dosage 10 000 U on promoting skin wound healing was declined. Conclusions The oxidative stress may playan important role in the development of wound healing.Proper application of treatment with antioxidants is a alternative strategy in the early stage of wound.PTD-SOD is able to prevent the oxidative stress damage,inhibit inflammatory infiltration and promote skin wound healing efficiently.
