中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2008年
10期
768-771
,共4页
李学松%龚侃%孙国锋%周利群%何志嵩%辛殿琪%那彦群%梁云燕%王代树%郭应禄
李學鬆%龔侃%孫國鋒%週利群%何誌嵩%辛殿琪%那彥群%樑雲燕%王代樹%郭應祿
리학송%공간%손국봉%주리군%하지숭%신전기%나언군%량운연%왕대수%곽응록
前列腺肿瘤%细胞周期%凋亡%Bcl-2
前列腺腫瘤%細胞週期%凋亡%Bcl-2
전렬선종류%세포주기%조망%Bcl-2
Prostate neoplasms%Cell cycle%Apoptosis%Bcl-2
目的 探讨凋亡相关基因Bel-2和Bax在前列腺癌不同周期时相的差异表达及意义.方法 应用改良的胸腺嘧啶核苷和高压笑气双阻断法把前列腺癌细胞系PC-3同步在不同的周期时相,应用流式细胞术检测同步化的效率.进而应用RT-PCR及Western blot方法检测不同周期时相Bcl-2和Bax在mRNA和蛋白水平的表达.结果 前列腺癌细胞系PC-3的M、G1、S和G2期细胞同步化效率分别为92.1%、87.0%、80.2%和75.9%;Bcl-2在不同周期时相均有表达且存在差异,G1期表达水平最高,S、M和G2期表达水平显著降低,mRNA和蛋白水平表达具有一致性;Bax在各周期的mRNA水平及蛋自水平表达没有明显差别.结论 细胞周期对凋亡相关基因Bel-2的表达有显著影响,对Bax无影响,对于采用Bcl-2途径治疗前列腺癌具有指导意义.
目的 探討凋亡相關基因Bel-2和Bax在前列腺癌不同週期時相的差異錶達及意義.方法 應用改良的胸腺嘧啶覈苷和高壓笑氣雙阻斷法把前列腺癌細胞繫PC-3同步在不同的週期時相,應用流式細胞術檢測同步化的效率.進而應用RT-PCR及Western blot方法檢測不同週期時相Bcl-2和Bax在mRNA和蛋白水平的錶達.結果 前列腺癌細胞繫PC-3的M、G1、S和G2期細胞同步化效率分彆為92.1%、87.0%、80.2%和75.9%;Bcl-2在不同週期時相均有錶達且存在差異,G1期錶達水平最高,S、M和G2期錶達水平顯著降低,mRNA和蛋白水平錶達具有一緻性;Bax在各週期的mRNA水平及蛋自水平錶達沒有明顯差彆.結論 細胞週期對凋亡相關基因Bel-2的錶達有顯著影響,對Bax無影響,對于採用Bcl-2途徑治療前列腺癌具有指導意義.
목적 탐토조망상관기인Bel-2화Bax재전렬선암불동주기시상적차이표체급의의.방법 응용개량적흉선밀정핵감화고압소기쌍조단법파전렬선암세포계PC-3동보재불동적주기시상,응용류식세포술검측동보화적효솔.진이응용RT-PCR급Western blot방법검측불동주기시상Bcl-2화Bax재mRNA화단백수평적표체.결과 전렬선암세포계PC-3적M、G1、S화G2기세포동보화효솔분별위92.1%、87.0%、80.2%화75.9%;Bcl-2재불동주기시상균유표체차존재차이,G1기표체수평최고,S、M화G2기표체수평현저강저,mRNA화단백수평표체구유일치성;Bax재각주기적mRNA수평급단자수평표체몰유명현차별.결론 세포주기대조망상관기인Bel-2적표체유현저영향,대Bax무영향,대우채용Bcl-2도경치료전렬선암구유지도의의.
Objective To investigate the differential expression of apoptosis associated gene Bcl-2 and Bax through cell cycle and its possible clinical meaning.Methods The prostate cancer cell line PC-3 was synchronized in M,G1,S and G2 phase using modified thymine deoxyriboside blockage and high pressure N2O technique.The efficiency of synchronization was detected by flow-cytometry.RT-PCR and western blot methods were used to examine the expression of Bcl-2 and Bax in mRNA and protein level.Results The synchronized rate of M,G1,S and G,phase were 92.1%,87.0%,80.2% and 75.9%respectively.Bcl-2 was constitutively expressed through the cell cycle,but both the mRNA and protein expression level of Bcl-2 were very high in the G1 phase,dramatically decreased in M,S and G2 phase.The expression level of Bax had no change through the cell cycle.Conclusions Cell cycle could influence the expression level of Bcl-2 significantly but not Bax.these might have some clinical relevance.
