CAJ | 학술논문

DNA甲基化作为一种重要的表观遗传修饰,广泛存在于高等动植物中,并在维持基因组稳定性、调节基因表达等方面起着重要作用,因此建立快速有效地DNA甲基化检测技术至关重要.本文以两种不同MuDR活性的玉米转座子材料为研究对象, 探讨了甲基化特异性PCR(MSP)在检测DNA甲基化的有效性.结果表明: MSP技术可快速有效地检测MuDR转座子的末端反向重复(TIRs)序列内的CpG岛DNA甲基化的变化,灵敏度高,特异性强,可作为植物已知基因DNA甲基化检测的一种新方法.同时利用MSP研究发现,玉米MuDR转座子的活性随其TIRs序列内的CpG岛DNA甲基化的变化而改变, DNA甲基化是调控玉米MuDR转座活性的重要分子机制之一.
DNA갑기화작위일충중요적표관유전수식,엄범존재우고등동식물중,병재유지기인조은정성、조절기인표체등방면기착중요작용,인차건립쾌속유효지DNA갑기화검측기술지관중요.본문이량충불동MuDR활성적옥미전좌자재료위연구대상, 탐토료갑기화특이성PCR(MSP)재검측DNA갑기화적유효성.결과표명: MSP기술가쾌속유효지검측MuDR전좌자적말단반향중복(TIRs)서렬내적CpG도DNA갑기화적변화,령민도고,특이성강,가작위식물이지기인DNA갑기화검측적일충신방법.동시이용MSP연구발현,옥미MuDR전좌자적활성수기TIRs서렬내적CpG도DNA갑기화적변화이개변, DNA갑기화시조공옥미MuDR전좌활성적중요분자궤제지일.
DNA methylation is a kind of important epigenetic modification, which widely exists in the higher animals and plants and plays an influential role in maintaining genomic stability and regulating gene expression. Thus, it is very vital to develop a rapid and effective technique of detecting DNA methylation. In this thesis, two Mutator lines with different MuDR activity were utilized to explore the MSP validity in determining DNA methylation status. The results indicated that by using the Methylation-specific PCR (MSP) approach, it is feasible to determine the methylation status of CpG islands of terminal inverted repeats (TIRs) of MuDR element rapidly and effectively. Furthermore, this method also has a high sensitivity and intense specificity, so it can be used as a new method of detecting DNA methylation of the known plant genes. Meanwhile, this research has also revealed that based on MSP analysis, the variation of MuDR activity is associated with the alteration of methylation status of CpG islands of terminal inverted repeats (TIRs) of MuDR element in maize. Thus, DNA methylation may be one of important molecular mechanisms in regulating the MuDR transposition activity in Maize.