CAJ | 학술논문

目的:观察超声波辐照对培养的血管平滑肌细胞(VSMCs)增殖和凋亡的影响,筛选防止介入治疗后血管再狭窄的辐照参数,并探讨其机理.方法:单声源压电陶瓷超声辐照系统声强连续可调,台盼蓝排斥实验分析辐照后即刻细胞存活率;MTT比色法描述辐照后5天VSMCs的生长动力学变化;BrdU掺入法检测辐照对VSMCs S期DNA复制的影响;ELISA法检测辐照后VSMCs凋亡情况.结果:1.超声辐照对培养的VSMCs有直接杀伤作用,该作用随辐照强度和辐照时间的增加而增加,相同辐照强度和时间下,低频超声该作用更显著.2.超声辐照组较对照组VSMCs生长曲线平缓,0.705MHz组以0.5W/cm2,5min辐照最为明显.3.BrdU掺入法测定S期细胞增殖发现,0.705MHz,0.5W/cm2,5min辐照组BrdU%低于对照组(40.5%±6.8%vs55.8%±3.3%,P＜0 05).4.0.705MHz,0.5W/cm2,5min辐照的凋亡富积因子与阳性对照相比为1.851±0.087vs1.924±0.074,P=0.836.结论:0.705MHz,0.5W/cm2,5min超声辐照在无明显杀伤细胞的情况下诱导培养的VSMCs凋亡,阻止VSMCs S期DNA复制,提示USE是一种潜在的治疗介入治疗后血管再狭窄的手段.
목적:관찰초성파복조대배양적혈관평활기세포(VSMCs)증식화조망적영향,사선방지개입치료후혈관재협착적복조삼수,병탐토기궤리.방법:단성원압전도자초성복조계통성강련속가조,태반람배척실험분석복조후즉각세포존활솔;MTT비색법묘술복조후5천VSMCs적생장동역학변화;BrdU참입법검측복조대VSMCs S기DNA복제적영향;ELISA법검측복조후VSMCs조망정황.결과:1.초성복조대배양적VSMCs유직접살상작용,해작용수복조강도화복조시간적증가이증가,상동복조강도화시간하,저빈초성해작용경현저.2.초성복조조교대조조VSMCs생장곡선평완,0.705MHz조이0.5W/cm2,5min복조최위명현.3.BrdU참입법측정S기세포증식발현,0.705MHz,0.5W/cm2,5min복조조BrdU%저우대조조(40.5%±6.8%vs55.8%±3.3%,P＜0 05).4.0.705MHz,0.5W/cm2,5min복조적조망부적인자여양성대조상비위1.851±0.087vs1.924±0.074,P=0.836.결론:0.705MHz,0.5W/cm2,5min초성복조재무명현살상세포적정황하유도배양적VSMCs조망,조지VSMCs S기DNA복제,제시USE시일충잠재적치료개입치료후혈관재협착적수단.