北京中医药大学学报
北京中醫藥大學學報
북경중의약대학학보
JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2010年
3期
187-190,后插二
,共5页
张燕%李红岩%郭伶俐%孙令军
張燕%李紅巖%郭伶俐%孫令軍
장연%리홍암%곽령리%손령군
慢传输型便秘%Cajal间质细胞%血管活性肠肽%大黄%大鼠
慢傳輸型便祕%Cajal間質細胞%血管活性腸肽%大黃%大鼠
만전수형편비%Cajal간질세포%혈관활성장태%대황%대서
slow transit constipation%interstitial Cajal cells%vasoactive intestinal polypeptide%Dahuang(Radix et Rhizoma Rhei)%rats
目的 观察大黄对慢传输型便秘大鼠结肠传输能力的影响,揭示大黄与该病发展的相关性.方法 将健康SD大鼠24只随机分为大黄组及对照组,大黄组应用中药大黄喂养制作慢传输型便秘的动物模型.分别于禁食后,经口灌入活性炭悬液10 mL/kg,动物处死后剖腹取出幽门到直肠末段的全部肠管并测量肠管长度、碳末推进长度及肠道传输功能.取结肠组织进行HE染色及电镜下观察.同时进行免疫组织化学染色,观察血管活性肠肽(VIP)的表达状况.结果 大黄组碳末推进长度、肠道传输功能低于对照组.大黄组大鼠结肠肌间神经丛VIP含量较对照组明显下降,光镜及电镜下均可见结肠细胞结构明显退行性改变,结肠肌间神经丛内未找到Cajal间质细胞(ICC).结论 大黄等蒽醌类泻剂损伤结肠壁神经丛,使结肠传输功能下降.因此,对慢传输型便秘患者治疗时,应避免长期应用此类药物.
目的 觀察大黃對慢傳輸型便祕大鼠結腸傳輸能力的影響,揭示大黃與該病髮展的相關性.方法 將健康SD大鼠24隻隨機分為大黃組及對照組,大黃組應用中藥大黃餵養製作慢傳輸型便祕的動物模型.分彆于禁食後,經口灌入活性炭懸液10 mL/kg,動物處死後剖腹取齣幽門到直腸末段的全部腸管併測量腸管長度、碳末推進長度及腸道傳輸功能.取結腸組織進行HE染色及電鏡下觀察.同時進行免疫組織化學染色,觀察血管活性腸肽(VIP)的錶達狀況.結果 大黃組碳末推進長度、腸道傳輸功能低于對照組.大黃組大鼠結腸肌間神經叢VIP含量較對照組明顯下降,光鏡及電鏡下均可見結腸細胞結構明顯退行性改變,結腸肌間神經叢內未找到Cajal間質細胞(ICC).結論 大黃等蒽醌類瀉劑損傷結腸壁神經叢,使結腸傳輸功能下降.因此,對慢傳輸型便祕患者治療時,應避免長期應用此類藥物.
목적 관찰대황대만전수형편비대서결장전수능력적영향,게시대황여해병발전적상관성.방법 장건강SD대서24지수궤분위대황조급대조조,대황조응용중약대황위양제작만전수형편비적동물모형.분별우금식후,경구관입활성탄현액10 mL/kg,동물처사후부복취출유문도직장말단적전부장관병측량장관장도、탄말추진장도급장도전수공능.취결장조직진행HE염색급전경하관찰.동시진행면역조직화학염색,관찰혈관활성장태(VIP)적표체상황.결과 대황조탄말추진장도、장도전수공능저우대조조.대황조대서결장기간신경총VIP함량교대조조명현하강,광경급전경하균가견결장세포결구명현퇴행성개변,결장기간신경총내미조도Cajal간질세포(ICC).결론 대황등은곤류사제손상결장벽신경총,사결장전수공능하강.인차,대만전수형편비환자치료시,응피면장기응용차류약물.
Objective To observe the influence of Dahuang(Radix et Rhizoma Rhei)on colonic transit in rats with slow transit constipation(STC),and reveal the correlation between Dahuang and STC development.Methods All SD rats(n=24)were randomly divided into the Dahuang group and control group.The rat model of STC was established by feeding Dahuang in the Dahuang group,and after fasting activated charcoal suspension(10 mL/kg)were given orally to rats of two groups respectively.The length of whole intestine,charcoal powder propelling length and colonic transit were detected after killing rats and taking the intestine from pylorus to distal rectum.The colonic tissue was stained by HE and observed with the electron microscope,and at the same time stained by immunohistochemistry for observing the expression of vasoactive intestinal polypeptide(VIP).Results The charcoal powder propelling length and colonic transit were reduced in the Dahuang group compared with those in the control group.The level of VIP in colonic intermuscular nerve plexus decreased significantly in the Dahuang group than that in the control group.The obvious retrograde degenerations of colonic cytoarchitacture were observed by both light microscope and electron microscope.The colonic transit was slower and there was no interstitial Cajal cells (ICC)in colonic intermuscular nerve plexus in the Dahuang group,and VIP level decreased significantly compared with that in the control group.Conclusion Anthraquinone cathartic,including Dahuang,can injure colonic intermuscular nerve plexus and induce colonic transit.This kind of medicines should not be administrated for long time when curing STC.
