CAJ | 학술논문

目的研究文冠果壳苷对侧脑室注射β肽淀粉样蛋白_(1-42)(Aβ_(1-42))小鼠学习记忆障碍的改善作用,并初步探讨了其作用机制.方法通过避暗和水迷宫实验检测了小鼠的学习记忆能力;使用分光光度计检测小鼠大脑组织中乙酰胆碱酯酶(AchE)和胆碱乙酰转移酶(ChAT)活性的变化.结果小鼠侧脑室注射凝聚态Aβ(1-42)显著降低了小鼠被动回避学习记忆能力及空间学习记忆能力(P<0.01),并且小鼠大脑AchE和ChAT活性显著降低(P均<0.05);而文冠果壳苷能够显著减少避暗错误次数,延长潜伏期(分别P<0.05、P<0.01);剂量依赖性地缩短水迷宫实验中小鼠到达安全台的游泳时间(分别P<0.05、P<0.01),不同程度地抑制Aβ_(1-42)模型小鼠的AchE和ChAT活性的降低(P<0.05和<0.01).结论文冠果壳苷对侧脑室注射Aβ_(1-42)小鼠学习记忆障碍具有显著的改善作用,其作用机制可能与保护中枢胆碱能神经细胞,改善中枢胆碱能神经系统功能等有关.
목적 연구문관과각감대측뇌실주사β태정분양단백_(1-42)(Aβ_(1-42))소서학습기억장애적개선작용,병초보탐토료기작용궤제.방법 통과피암화수미궁실험검측료소서적학습기억능력;사용분광광도계검측소서대뇌조직중을선담감지매(AchE)화담감을선전이매(ChAT)활성적변화.결과 소서측뇌실주사응취태Aβ(1-42)현저강저료소서피동회피학습기억능력급공간학습기억능력(P<0.01),병차소서대뇌AchE화ChAT활성현저강저(P균<0.05);이문관과각감능구현저감소피암착오차수,연장잠복기(분별P<0.05、P<0.01);제량의뢰성지축단수미궁실험중소서도체안전태적유영시간(분별P<0.05、P<0.01),불동정도지억제Aβ_(1-42)모형소서적AchE화ChAT활성적강저(P<0.05화<0.01).결론 문관과각감대측뇌실주사Aβ_(1-42)소서학습기억장애구유현저적개선작용,기작용궤제가능여보호중추담감능신경세포,개선중추담감능신경계통공능등유관.
Objective To investigate the effects of Xanthoceraside on the learning and memory impairment induced in mire by innacere-broventricular injection of aggregated amyloid β peptide _(1-42)(Aβ_(1-42)). Methods Learning and memory functions in mice were examined us-ing step-through test and water maze test. Biochemical determination of acetylcholinesterase (AchE) and choline acetyl transferase (ChAT) were measured with spectrophotometric melhod. Results Administration of Xanthoceraside reduced number of error and prolonged the laten-cy in step-through test in mice impaired by Aβ_(1-42) (P < 0.05,P< 0.01,respectively). In water maze test,the swimming time decreased in mice treated with Xanthoceraside compared with the model mice impaired by Aβ_(1-42) (P< 0.05,P< 0.01,respectively). The results of bio-chemical determination showed that decrease level of AchE and ChAT in mice impaired by Aβ_(1-42) were significantly ameliorated by Xantho-ceraside administration (P < 0.05 and P < 0.01). Conclusion Xanthoceraside has the effect of improving learning and memory impairment in mice inducel by intracerebroventricular injection of Aβ_(1-42) via enhancing the cholinergic system functions.