CAJ | 학술논문

目的:探讨降钙素基因相关肽(CGRP)对脂多糖(LPS)诱导的小鼠巨噬细胞表达髓样细胞触发受体-1(TREM-1)的影响及其信号转导途径.方法:采用反转录-聚合酶链式反应(RT-PCR)观察巨噬细胞TREM-1 mRNA表达量的变化,应用流式细胞术检测巨噬细胞膜表面TREM-1蛋白的表达.(结果):CGRP对未受刺激的巨噬细胞表达TREM-1无明显影响,LPS可诱导巨噬细胞表达TREM-1;CGRP预处理呈剂量和时间依赖性上调LPS所致的巨噬细胞TREM-1 mRNA的表达;同时,CGRP上调LPS所致的巨噬细胞膜表面TREM-1蛋白的表达;上述作用均可被PKC阻断剂H-7和PKA阻断剂H-89部分逆转(P<0.05).结论:CGRP上调LPS诱导的巨噬细胞表达TREM-1,其胞内信号转导途径与PKA和PKC有关.
목적:탐토강개소기인상관태(CGRP)대지다당(LPS)유도적소서거서세포표체수양세포촉발수체-1(TREM-1)적영향급기신호전도도경.방법:채용반전록-취합매련식반응(RT-PCR)관찰거서세포TREM-1 mRNA표체량적변화,응용류식세포술검측거서세포막표면TREM-1단백적표체.(결과):CGRP대미수자격적거서세포표체TREM-1무명현영향,LPS가유도거서세포표체TREM-1;CGRP예처리정제량화시간의뢰성상조LPS소치적거서세포TREM-1 mRNA적표체;동시,CGRP상조LPS소치적거서세포막표면TREM-1단백적표체;상술작용균가피PKC조단제H-7화PKA조단제H-89부분역전(P<0.05).결론:CGRP상조LPS유도적거서세포표체TREM-1,기포내신호전도도경여PKA화PKC유관.
Objective To determine the effect of calcitonin gene-related peptide (CGRP) on triggering receptor expressed on myeloid cells-1 (TREM-1) in the lipopolysaccharide (LPS)-induced macrophages and its signal transduction pathway. Methods The levels of TREM-1 mRNA in the macrophages were observed by reverse transcription-polymerase chain reaction (RT-PCR), and flow cytometry was performed to detect TREM-1 protein expression levels in the macrophages. Results CGRP had no regulating effect on the expression of TREM-1 in the macrophages; LPS could up-regulate macrophages to express TREM-1; CGRP increased TREM-1 mRNA expression in LPS-induced macrophages in dose and time-dependent manner; CGRP increased TREM-1 protein expression in LPS-induced macrophages, which could be partially reversed by H-7 or H-89 (P<0.05). Conclusion CGRP can regulate the LPS-induced macrophages synthesis and secretion of TREM-1, and the intracellular signal transduction pathway is related to PKA and PKC.