CAJ | 학술논문

利用随机扩增多态性DNA(RAPD)标记对7个猴头菇之间的亲缘关系进行研究,获得了猴头菇不同菌株的DNA指纹图谱.结果显示:14个随机引物中有3个随机引物的扩增产物的DNA条带表现出明显的多态性.这3个引物共扩增出44条带,其中36条为多态性条带,多态性位点比率为81.81%.同时利用NTSYSpc 2.1生物软件分析7个供试菌株之间的遗传相似性,并绘制系统进化树.
이용수궤확증다태성DNA(RAPD)표기대7개후두고지간적친연관계진행연구,획득료후두고불동균주적DNA지문도보.결과현시:14개수궤인물중유3개수궤인물적확증산물적DNA조대표현출명현적다태성.저3개인물공확증출44조대,기중36조위다태성조대,다태성위점비솔위81.81%.동시이용NTSYSpc 2.1생물연건분석7개공시균주지간적유전상사성,병회제계통진화수.
The phylogenetic relationship of 7 Hericium erinaceuss strains was studied using randomly amplified polymorphic DNA (RAPD) markers. The DNA fingerprints of different strains showed that there was genetic diversity among the tested strains. The results indicated that 3 out of 14 random primers were polymorphic. These 3 random primers amplified 44 bands in total, 36 bands of which showed polymorphism and the polymorphic rate was 81.81%. Furthermore, the NTSYSpc 2.1 biology software was used to analyze the genetic similarity of the tested strains and to construct the phylogenetic tree. This study can provide the molecular evidences for variety identification and phylogenetic analysis of the Hericium erinaceus.