湖北农业科学
湖北農業科學
호북농업과학
2010年
2期
412-415,442
,共5页
鲫鱼%氰戊菊酯%肝胰脏%鳃%超氧化物歧化酶(SOD)%碱性磷酸酶(AKP)%酸性磷酸酶(AKP)%谷光甘肽硫转移酶(GSTs)
鯽魚%氰戊菊酯%肝胰髒%鰓%超氧化物歧化酶(SOD)%堿性燐痠酶(AKP)%痠性燐痠酶(AKP)%穀光甘肽硫轉移酶(GSTs)
즉어%청무국지%간이장%새%초양화물기화매(SOD)%감성린산매(AKP)%산성린산매(AKP)%곡광감태류전이매(GSTs)
Carassius auratus%fenvalerate%liver%gill%superoxide dismutase (SOD)%alkaline phosphatase (AKP)%acid pbosphatase (ACP)%glutathione S-transferases(GSTs)
为了探讨氰戊菊酯对鲫鱼的影响,用不同浓度的氰戊菊酯(5、10、20、40μg/L)分别处理鲫鱼4、8、12、16d后,对肝胰脏、鳃中的超氧化物歧化酶(SOD)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、谷胱甘肽硫转移酶(GSTs)活性的变化进行了检测.结果表明,低浓度的氰戊菊酯能激活鲫鱼肝胰脏和鳃中的超氧化物歧化酶(SOD)活性,但随着氰戊菊酯浓度的增加和时间的延长,SOD酶活性受到不同程度的抑制;肝胰脏和鳃中AKP和ACP酶活性均随氰戊菊酯浓度的增加和染毒时间的延长而降低.其中在染毒后4d,肝胰脏AKP酶活性随着氯戊菊酯农度升高而显著或极显著降低.肝胰脏ACP酶活性在染毒后12d和16d,试验组与对照组差异显著.鳃组织ACP活性试验组与对照组相比,大部分没有显著差异.氰戊菊酯对鲫鱼肝胰脏GSTs活性产生较强的影响,存在剂量-效应和时间-效应关系.鲫鱼肝姨脏和鳃中的SOD、AKP,肝胰脏中ACP、GSTs活性均可以用作其组织细胞受农药胁迫的生物标志物,鲫鱼可作为一种淡水水体污染的监测生物.
為瞭探討氰戊菊酯對鯽魚的影響,用不同濃度的氰戊菊酯(5、10、20、40μg/L)分彆處理鯽魚4、8、12、16d後,對肝胰髒、鰓中的超氧化物歧化酶(SOD)、堿性燐痠酶(AKP)、痠性燐痠酶(ACP)、穀胱甘肽硫轉移酶(GSTs)活性的變化進行瞭檢測.結果錶明,低濃度的氰戊菊酯能激活鯽魚肝胰髒和鰓中的超氧化物歧化酶(SOD)活性,但隨著氰戊菊酯濃度的增加和時間的延長,SOD酶活性受到不同程度的抑製;肝胰髒和鰓中AKP和ACP酶活性均隨氰戊菊酯濃度的增加和染毒時間的延長而降低.其中在染毒後4d,肝胰髒AKP酶活性隨著氯戊菊酯農度升高而顯著或極顯著降低.肝胰髒ACP酶活性在染毒後12d和16d,試驗組與對照組差異顯著.鰓組織ACP活性試驗組與對照組相比,大部分沒有顯著差異.氰戊菊酯對鯽魚肝胰髒GSTs活性產生較彊的影響,存在劑量-效應和時間-效應關繫.鯽魚肝姨髒和鰓中的SOD、AKP,肝胰髒中ACP、GSTs活性均可以用作其組織細胞受農藥脅迫的生物標誌物,鯽魚可作為一種淡水水體汙染的鑑測生物.
위료탐토청무국지대즉어적영향,용불동농도적청무국지(5、10、20、40μg/L)분별처리즉어4、8、12、16d후,대간이장、새중적초양화물기화매(SOD)、감성린산매(AKP)、산성린산매(ACP)、곡광감태류전이매(GSTs)활성적변화진행료검측.결과표명,저농도적청무국지능격활즉어간이장화새중적초양화물기화매(SOD)활성,단수착청무국지농도적증가화시간적연장,SOD매활성수도불동정도적억제;간이장화새중AKP화ACP매활성균수청무국지농도적증가화염독시간적연장이강저.기중재염독후4d,간이장AKP매활성수착록무국지농도승고이현저혹겁현저강저.간이장ACP매활성재염독후12d화16d,시험조여대조조차이현저.새조직ACP활성시험조여대조조상비,대부분몰유현저차이.청무국지대즉어간이장GSTs활성산생교강적영향,존재제량-효응화시간-효응관계.즉어간이장화새중적SOD、AKP,간이장중ACP、GSTs활성균가이용작기조직세포수농약협박적생물표지물,즉어가작위일충담수수체오염적감측생물.
The effects of different concentration of fenvalerate (5,10,20,40μg/L)on activities of superoxide dismutase (SOD),alkaline phosphatase (AKP),acid phosphatase (ACP) and glutathione S-transferases(GSTs) were investigated in liver and gill of crucian(Carassius auratus) for 4,8,12 and 16 days after exposure.The results showed that the activities of SOD increased in low fenvalerate levels.Then the activities of SOD decreased as the increase in fenvalerate concentration with the time extension.The AKP and ACP activities in liver and gill decreased as the increase in fenvalerate concentration with the time extension.After 4d,the activities of AKP in liver decreased obviously with the concentration enhancing (P＜0.05).After 12 and 16d,the activities of ACP in liver significantly reduced(P＜0.05).While ACP activities in gill showed no significantly difference (P＞0.05).Meanwhile,GSTs activities in liver and gill changed obviously with the fenvalerate contamination.The time-response and dose-response relationship existed between fenvalerate and activities of GSTs in liver and gill.The results suggested that fenvalerate could induce the activities of enzymes related with immune.Under the laboratory simulating conditions,the inchoate changes in SOD,AKP,ACP,GSTs activity in liver and gill could be regarded as sensitive indices of fenvalerate to the immune toxicity to the crucian (Carassius auratus) and Carasslus auratu could serve as an indicating organism of fresh water pollution environment.
