CAJ | 학술논문

背景:从蚕丝中提取的丝素蛋白具有良好的细胞相容性,且可生物降解,用于修饰生物材料能提高细胞在材料表面的黏附和生长能力.目的:探讨丝素蛋白,羟基磷灰石材料复合脂肪间充质干细胞修复包含性骨缺损的作用.方法:取2月龄新西兰大白兔附睾处脂肪组织,经胰酶消化传代培养脂肪间充质干细胞,取第3代兔脂肪问充质干细胞以1 ×10~(10)L~(-1)浓度接种于丝素蛋白/羟基磷灰支架材料上,培养3h后加入含有1 μmol/L地塞米松、50 pmol/L维生素C、10 mmol/Lβ-甘油磷酸钠的DMEM培养液进行成骨诱导.新西兰大白兔36只,在兔股骨远端制备直径4.5 mm、深10mm的松质骨缺损.细胞复合材料组植入复合脂肪间充质干细胞的丝素蛋白,羟基磷灰石;单纯材料组植入丝素蛋白,羟基磷灰石;空白对照组不作任何植入.结果与结论:12周时大体观察细胞复合材料组骨缺损区完全被骨组织修复,单纯材料组骨缺损区缩小、部分修复,空白对照组骨缺损无修复.12周时X射线、组织学检查显示细胞复合材料组完全修复了骨缺损区,材料组部分修复了骨缺损区,细胞复合材料组的新生骨小梁多于单纯材料组(P<0.05),空白对照组未见骨修复.结果说明复合脂肪间充质干细胞的丝素蛋白,羟基磷灰石修复兔股骨包含性骨缺损能力优于单纯丝素蛋白,羟基磷灰石材料.
배경:종잠사중제취적사소단백구유량호적세포상용성,차가생물강해,용우수식생물재료능제고세포재재료표면적점부화생장능력.목적:탐토사소단백,간기린회석재료복합지방간충질간세포수복포함성골결손적작용.방법:취2월령신서란대백토부고처지방조직,경이매소화전대배양지방간충질간세포,취제3대토지방문충질간세포이1 ×10~(10)L~(-1)농도접충우사소단백/간기린회지가재료상,배양3h후가입함유1 μmol/L지새미송、50 pmol/L유생소C、10 mmol/Lβ-감유린산납적DMEM배양액진행성골유도.신서란대백토36지,재토고골원단제비직경4.5 mm、심10mm적송질골결손.세포복합재료조식입복합지방간충질간세포적사소단백,간기린회석;단순재료조식입사소단백,간기린회석;공백대조조불작임하식입.결과여결론:12주시대체관찰세포복합재료조골결손구완전피골조직수복,단순재료조골결손구축소、부분수복,공백대조조골결손무수복.12주시X사선、조직학검사현시세포복합재료조완전수복료골결손구,재료조부분수복료골결손구,세포복합재료조적신생골소량다우단순재료조(P<0.05),공백대조조미견골수복.결과설명복합지방간충질간세포적사소단백,간기린회석수복토고골포함성골결손능력우우단순사소단백,간기린회석재료.
BACKGROUND: Silk fibroin derived from silk had a good biocompatibility and biodegradation, which could be used for biomaterials to improve cell adhesion and growth abilities. OBJECTIVE: To investigate the efficacy of silk fibroin/hydroxyapatite (SF/HA) compounded of adipose-derived stromal cells (ADSCs) on repairing bone defects. METHODS: Adipose tissues were derived from epididymis of 2-month-old New Zealand rabbits and trypsogen-passaged to obtain ADSCs. The third-passage ADSCs at the concentration of 1×10/L were placed on SF/HA scaffold. Three hours later, the composite was cultured with DMEM culture media containing 1 μmol/L dexamethasone, 50 μmol/L vitamin C, and 10 mmol/L β-sodium glycerophosphate. Thirty-six rabbits were induced cancellated bone defect sizing 4.5 mm × 4.5 mm × 10 mm. The composite group was implanted with SF/HN/ADSCs scaffold, the simple group was implanted with SF/HA scaffold, but any treatment was employed in the blank control group. RESULTS AND CONCLUSION: At 12 weeks, general observation demonstrated that the bone defects were repaired entirely in composite group and partly in simple group. However, the bone defect was not repaired in the blank control group. X-ray and histological observation suggested that at 12 weeks the bone defects were repaired entirely in composite group and partly in simple group. The quantity of the newly formed bone in the composite group was significantly more than that in the simple group (P < 0.05). Repair showed no effect in the blank control group. SF/HA/ADSCs composite could successfully repair bone defects of a rabbit femur, and the effect was superior to SF/HA scaffold.