CAJ | 학술논문

基于元分析的大豆胞囊线虫抗性QTL的整合
기우원분석적대두포낭선충항성QTL적정합
Integration of QTLs related to soybean cyst nematode resistance based on meta-analysis

万方数据

中国油料作物学报中國油料作物學報 중국유료작물학보
CHINESE JOURNAL OF OIL CROP SCIENCES
2010年 1期 104-109 ,共6页

张闻博%蒋洪蔚%李灿东%邱鹏程%齐照明%刘春燕%姜威%王晶%胡国华%陈庆山

장문박%장홍위%리찬동%구붕정%제조명%류춘연%강위%왕정%호국화%진경산

大豆胞囊线虫%QTL的映射%元分析%真实QTL 大豆胞囊線蟲%QTL的映射%元分析%真實QTL
대두포낭선충%QTL적영사%원분석%진실QTL
SCN%QTL projection%Meta-analysis%Real QTL

以2004年发布的大豆公共遗传图谱soymap2为参考图谱,共搜集整理了自1994年以来已报道的与抗大豆胞囊线虫相关的151个QTLs,通过BioMercator2.1和公共标记将相关QTLs映射整合到大豆公共遗传连锁图谱soymap2上,并利用元分析技术发掘"真实"QTL.本文共发掘出与抗大豆胞囊线虫1、2、3、4、5和14号生理小种相关的16个"真实"QTL,其中有四个位点的图距小于1.5cM,一个位点的图距小于5cM,分布于A2、E和G连锁群,其中G连锁群上5.11cM处的定位区间包含已报道的Rhg1位点;发现在G连锁群上有一个定位区间控制1,4号生理小种,在B1连锁群上有一个定位区间控制2,5号生理小种,可见这些位点具有兼抗性.
이2004년발포적대두공공유전도보soymap2위삼고도보,공수집정리료자1994년이래이보도적여항대두포낭선충상관적151개QTLs,통과BioMercator2.1화공공표기장상관QTLs영사정합도대두공공유전련쇄도보soymap2상,병이용원분석기술발굴"진실"QTL.본문공발굴출여항대두포낭선충1、2、3、4、5화14호생리소충상관적16개"진실"QTL,기중유사개위점적도거소우1.5cM,일개위점적도거소우5cM,분포우A2、E화G련쇄군,기중G련쇄군상5.11cM처적정위구간포함이보도적Rhg1위점;발현재G련쇄군상유일개정위구간공제1,4호생리소충,재B1련쇄군상유일개정위구간공제2,5호생리소충,가견저사위점구유겸항성.
According to soybean public map soymap2 (published in 2004), 151 QTLs related to soybean cyst nematode resistance were collected from markers reported since 1994. They were integrated and projected to the reference soymap2 using BioMercator2.1 software. 16"real" QTLs and their corresponding markers were obtained using meta-analysis method to narrow down the confidence interval. The "real" QTLs included markers related cyst nematode resistance to race 1,2,3,4,5, and 14. Part of them distributed on A2, E, and G linkage groups (LGs). Four of them had intervals less than 1.5 cM and one of them less than 5 cM. Rhg1,a previously reported locus, was found on LG G with 5.11 cM. Two of them located on LG B1 which related to race 2 and 5, another two on LG G related to race1 and 4. These results indicated double resistances to soybean cyst nematode and offered a basis for gene mining or molecular breeding in soybean.