CAJ | 학술논문

运用移动反应界面理论建立奶粉中三聚氰胺的毛细管电泳富集分离体系。与传统的毛细管电泳相比,体系中引入了富集缓冲溶液（富集相）和分离缓冲溶液（分离相）。优化的条件：样品缓冲溶液为50mmol/L甲酸-甲酸钠（pH2.60）,富集相为20mmol/L甲酸钠-甲酸pH8.00）,分离缓冲溶液为50mmol/L～50mmol/L甲酸-甲酸钠（pH2.60）;样品压力进样50mbar×40s,富集相压力进样50mbar×20s,紫外检测波长232nm,电压30kv。该方法比常规电泳灵敏度高近20倍。
운용이동반응계면이론건립내분중삼취청알적모세관전영부집분리체계。여전통적모세관전영상비,체계중인입료부집완충용액（부집상）화분리완충용액（분리상）。우화적조건：양품완충용액위50mmol/L갑산-갑산납（pH2.60）,부집상위20mmol/L갑산납-갑산pH8.00）,분리완충용액위50mmol/L～50mmol/L갑산-갑산납（pH2.60）;양품압력진양50mbar×40s,부집상압력진양50mbar×20s,자외검측파장232nm,전압30kv。해방법비상규전영령민도고근20배。
A stacking system was based on moving reaction boundary（MRB） for stacking and separation of melamine in milk powder.The experimental conditions were optimized for the stacking of melamine as well as its separation.The optimized conditions were 50mmol/L HCOOH-HCOONa（pH2.60） as sample buffer,20mmol/L HCOONa-HCOOH（pH8.00） as stacking buffer,50mmol/L HCOOH-HCOONa（pH 2.60） as separation buffer,50mbar（40s） sample phase injection and 50mbar（20s） stacking phase injection,232nm of detection wavelength,30kv of voltage.This method can be well used for stacking and separation of melamine in milk powder.