农业科学与技术：英文版
農業科學與技術：英文版
농업과학여기술：영문판
Agricultural Science ＆ Technology
2011年
6期
893-896
,共4页
罗格列酮%血清%脂肪形成%前脂肪细胞%基因表达%猪
囉格列酮%血清%脂肪形成%前脂肪細胞%基因錶達%豬
라격렬동%혈청%지방형성%전지방세포%기인표체%저
Rosiglitazone%Serum%Adipogenesis%Preadipocyte%Gene expression%Pig
[目的]探讨罗格列酮和血清对猪前脂肪细胞诱导分化过程中PPARα和PPARγ基因表达的影响。[方法]利用胶原酶消化法分离了猪皮下前脂肪细胞,采用3种不同分化培养液对猪前脂肪细胞进行了诱导分化,借助油红O染色提取法比较了不同分化培养液对分化过程中细胞内脂肪含量变化的影响,并运用实时定量RT-PCR方法检测了不同分化培养液细胞分化过程中PPARα和PPARγ基因表达的变化趋势。[结果]细胞内脂肪聚积以含罗格列酮的MⅡ最快,不含罗格列酮的MⅠ最慢。罗格列酮可极显著上调PPARγ基因的表达（P〈0.01）,而对PPARα基因的表达存在一定的抑制作用,但不显著。血清对PPARγ基因的表达有极显著的上调作用（P〈0.01）,而对PPARα基因的表达有极显著下调作用（P〈0.01）。[结论]罗格列酮可极大地促进PPARγ基因的表达,继而增进细胞内脂肪沉积;血清中可能存在PPARγ基因的激活剂,同时存在PPARα基因的抑制因子。
[目的]探討囉格列酮和血清對豬前脂肪細胞誘導分化過程中PPARα和PPARγ基因錶達的影響。[方法]利用膠原酶消化法分離瞭豬皮下前脂肪細胞,採用3種不同分化培養液對豬前脂肪細胞進行瞭誘導分化,藉助油紅O染色提取法比較瞭不同分化培養液對分化過程中細胞內脂肪含量變化的影響,併運用實時定量RT-PCR方法檢測瞭不同分化培養液細胞分化過程中PPARα和PPARγ基因錶達的變化趨勢。[結果]細胞內脂肪聚積以含囉格列酮的MⅡ最快,不含囉格列酮的MⅠ最慢。囉格列酮可極顯著上調PPARγ基因的錶達（P〈0.01）,而對PPARα基因的錶達存在一定的抑製作用,但不顯著。血清對PPARγ基因的錶達有極顯著的上調作用（P〈0.01）,而對PPARα基因的錶達有極顯著下調作用（P〈0.01）。[結論]囉格列酮可極大地促進PPARγ基因的錶達,繼而增進細胞內脂肪沉積;血清中可能存在PPARγ基因的激活劑,同時存在PPARα基因的抑製因子。
[목적]탐토라격렬동화혈청대저전지방세포유도분화과정중PPARα화PPARγ기인표체적영향。[방법]이용효원매소화법분리료저피하전지방세포,채용3충불동분화배양액대저전지방세포진행료유도분화,차조유홍O염색제취법비교료불동분화배양액대분화과정중세포내지방함량변화적영향,병운용실시정량RT-PCR방법검측료불동분화배양액세포분화과정중PPARα화PPARγ기인표체적변화추세。[결과]세포내지방취적이함라격렬동적MⅡ최쾌,불함라격렬동적MⅠ최만。라격렬동가겁현저상조PPARγ기인적표체（P〈0.01）,이대PPARα기인적표체존재일정적억제작용,단불현저。혈청대PPARγ기인적표체유겁현저적상조작용（P〈0.01）,이대PPARα기인적표체유겁현저하조작용（P〈0.01）。[결론]라격렬동가겁대지촉진PPARγ기인적표체,계이증진세포내지방침적;혈청중가능존재PPARγ기인적격활제,동시존재PPARα기인적억제인자。
[Objective] The research aimed to discuss the effects of rosiglitazone and serum on the expressions of PPARα and PPARγ genes in the induced differentiation process of pig preadipocyte.[Method] The pig preadipocyte was separated by using the collagenase digestion method.Three kinds of different differentiation culture solutions were used to induce the differentiation of pig preadipocyte.The oil red O staining extraction method was used to contrast the influences of different differentiation culture solutions on the variation of cellular fat content in the differentiation process.Moreover,the variation trends of PPARα and PPARγ expressions in the cellular differentiation process in the different differentiation culture solutions were detected by the real-time quantification PCR.[Result] The cellular fat accumulation was the fastest in MII which contained rosiglitazone and was the slowest in MI which didn＇t contain rosiglitazone.Rosiglitazone could significantly increase the expression of PPARγ gene（P0.01）,but had the certain inhibition effect on the expression of PPARα gene,which wasn＇t significant.The serum had the extremely significant up-regulation effect on the expression of PPARγ gene（P0.01）,but had the extremely significant down-regulation effect on the expression of PPARα gene（P0.01）.[Conclusion] Rosiglitazone could greatly promote the expression of PPARγ gene,which increased the cellular fat deposition.Maybe the activator of PPARγ gene existed in the serum,and the inhibitor of PPARα gene existed simultaneously.
