中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
2期
93-96
,共4页
壳聚糖%多孔%组织相容性%纤维包膜%血管增生
殼聚糖%多孔%組織相容性%纖維包膜%血管增生
각취당%다공%조직상용성%섬유포막%혈관증생
Chitosan%Porous%Histocompatibility%Fibrous capsule%Angiogenesis
目的 分析交联剂戊二醛对多孔壳聚糖膜孔隙率与孔径的影响,并探讨该多孔膜作为皮下植入式葡萄糖传感器保护膜的可行性.方法 通过致孔剂法利用硅胶和不同量的戊二醛制成多孔壳聚糖膜,并采用密度法和切片法分析其孔隙率与孔径等结构参数,扫描电子显微镜观察其表面形态.将无交联的多孔膜植入到9只SD大鼠皮下,第7、17、45天取材切片染色,观察组织形态学变化,定量分析多孔膜内外胶原沉积与血管增生密度.结果 戊二醛能提高多孔壳聚糖膜的孔隙率,5％交联度时孔隙率最大达76.2％/73.0％(密度法/切片法),但同时降低了多孔膜孔径.使用孔径最大的不交联多孔膜(38.5 μm)皮下植入,膜内胶原沉积含量由第7天的6.74％增加到第45天的22.5％,而Masson染色测量的膜内增生血管密度由0.37％增加到2.56％,与HE染色测量结果一致(由0.11％增加到1.65％).第45天时膜外胶原沉积含量38.3％,是膜内的1.7倍.结论 多孔壳聚糖膜能降低材料-组织界面纤维成分的致密性,增加血管增生,具有良好的组织相容性,有望应用于植入式葡萄糖传感器.
目的 分析交聯劑戊二醛對多孔殼聚糖膜孔隙率與孔徑的影響,併探討該多孔膜作為皮下植入式葡萄糖傳感器保護膜的可行性.方法 通過緻孔劑法利用硅膠和不同量的戊二醛製成多孔殼聚糖膜,併採用密度法和切片法分析其孔隙率與孔徑等結構參數,掃描電子顯微鏡觀察其錶麵形態.將無交聯的多孔膜植入到9隻SD大鼠皮下,第7、17、45天取材切片染色,觀察組織形態學變化,定量分析多孔膜內外膠原沉積與血管增生密度.結果 戊二醛能提高多孔殼聚糖膜的孔隙率,5％交聯度時孔隙率最大達76.2％/73.0％(密度法/切片法),但同時降低瞭多孔膜孔徑.使用孔徑最大的不交聯多孔膜(38.5 μm)皮下植入,膜內膠原沉積含量由第7天的6.74％增加到第45天的22.5％,而Masson染色測量的膜內增生血管密度由0.37％增加到2.56％,與HE染色測量結果一緻(由0.11％增加到1.65％).第45天時膜外膠原沉積含量38.3％,是膜內的1.7倍.結論 多孔殼聚糖膜能降低材料-組織界麵纖維成分的緻密性,增加血管增生,具有良好的組織相容性,有望應用于植入式葡萄糖傳感器.
목적 분석교련제무이철대다공각취당막공극솔여공경적영향,병탐토해다공막작위피하식입식포도당전감기보호막적가행성.방법 통과치공제법이용규효화불동량적무이철제성다공각취당막,병채용밀도법화절편법분석기공극솔여공경등결구삼수,소묘전자현미경관찰기표면형태.장무교련적다공막식입도9지SD대서피하,제7、17、45천취재절편염색,관찰조직형태학변화,정량분석다공막내외효원침적여혈관증생밀도.결과 무이철능제고다공각취당막적공극솔,5％교련도시공극솔최대체76.2％/73.0％(밀도법/절편법),단동시강저료다공막공경.사용공경최대적불교련다공막(38.5 μm)피하식입,막내효원침적함량유제7천적6.74％증가도제45천적22.5％,이Masson염색측량적막내증생혈관밀도유0.37％증가도2.56％,여HE염색측량결과일치(유0.11％증가도1.65％).제45천시막외효원침적함량38.3％,시막내적1.7배.결론 다공각취당막능강저재료-조직계면섬유성분적치밀성,증가혈관증생,구유량호적조직상용성,유망응용우식입식포도당전감기.
Objective To analyze the impact of glutaraldehyde crosslinker on porosity and pore size of porous chitosan membrane (PCSM) and to explore the feasibility of PCSM as the protective membrane of glucose sensor.Methods The PCSM was prepared by particle leaching method using silica gel and different amounts of glutaraldehyde.Density method and slice method were used to analyze the structural parameters of PCSM such as porosity and pore size and its surface morphology was observed with scanning electron microscope (SCM). Thereafter, the uncrosslinked porous membranes were subcutaneously transplanted into 9 SD rats and extracted to observe their histomorphological changes by section staining at the 7th,17th and 45th day after transplantation.Quantitative analysis was then performed on collagen deposition inside and outside of the porous membranes and vascular proliferation density.Results The glutaraldehyde could increase the PCSM porosity that could reach the maximum of 76.2％/73.0％ with 5％ degree of crosslinking (density method vs slice method),but it could also decrease the pore size of porous membrane.The uncrosslinked porous membranes with the largest pore size (38.5 μm) were used and subcutaneously transplanted,which resulted in an increase of intramembranous collagen deposition content from 6.74％ at the 7th day to 22.5％ at the 45th day.Moreover,the intramembranous proliferated capillary density was increased from 0.37％ to 2.56％ using Masson staining,which was consistent with the measured outcomes using HE staining (ranged from 0.11％ to 1.65％ ).At the 45th day,the content of collagen deposition outside of the membrane was 38.3％ that was 1.7 times of the intramembranous collagen deposition content.Conclusions PSCM may decrease the compactness fibrous composition of material-tissue interface but increase vascular proliferation,with favorable histocompatibility.Thus,PCSM appears promising in application in transplanted glucose sensor.
