中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2012年
6期
508-511
,共4页
周震%宋宛珊%王占奎%毛蕾%马妍%张玉莲
週震%宋宛珊%王佔奎%毛蕾%馬妍%張玉蓮
주진%송완산%왕점규%모뢰%마연%장옥련
化痰通络法%脑梗死%溶栓%血脑屏障%内皮细胞%形态学
化痰通絡法%腦梗死%溶栓%血腦屏障%內皮細胞%形態學
화담통락법%뇌경사%용전%혈뇌병장%내피세포%형태학
Huatan Tongluo%Infarction%Thrombolysis%Blood-brain barrier%Endothelial cells%Morphology
目的 观察化痰通络法对急性脑梗死大鼠重组组织型纤溶酶原激活物( rt-PA)溶栓后神经功能缺损评分、血脑屏障通透性及海马内皮细胞超微结构的影响,为化痰通络法防治急性脑梗死溶栓后出血转化提供实验依据.方法 选择健康雄性SD大鼠120只分为6组,即假手术组、模型组、rt-PA组、低剂量中药组、中剂量中药组、高剂量中药组(每组又分为6h、24h、3d、7d四个时相),采用自身栓子法制备大脑中动脉栓塞(MCAO)模型大鼠,rt-PA组及中药组分别给予尾静脉注入rt-PA及联合3种不同剂量化痰通络中药干预.根据不同观察时相对大鼠进行神经功能缺损评分(Bederson's评分),采用依文思蓝染色(EB染色)检测血脑屏障(BBB)通透性,并通过电镜对大脑海马缺血区内皮细胞超微结构进行观察.结果 1.Bederson's评分:与模型组相比,rt-PA组、低剂量中药组、中剂量中药组、高剂量中药组神经功能缺损评分明显降低(P＜0.01)；与rt-PA组相比,以rt-PA联合化痰通络法中剂量组、高剂量组神经功能缺损评分降低最为明显(P＜0.05).2.EB染色:与模型组相比,rt-PA组、低剂量中药组、中剂量中药组、高剂量中药组EB含量均明显减低(P＜0.01)；与rt-PA组[6h:(236.72±79.79) ng/g；24h:(229.72±22.18) ng/g；3d:(163.34±24.45)ng/g；7d:( 133.21±25.09) ng/g]相比,以化痰通络法联合rt-PA中剂量组[6h:(121.63±33.27) ng/g;24h:(153.78±60.67) ng/g;3d:(76.05±27.11 )ng/g;7d:(72.97±25.81) ng/g]、高剂量组[6h评分( 123.11±23.65) ng/g；24h评分(138.36±52.36) ng/g；3d评分(83.00±32.00) ng/g;7d评分(75.06±13.97) ng/g]EB含量降低最为明显(P＜0.01).3.电镜观察:与模型组相比,治疗组均起到保护内皮细胞的作用,其中以rt-PA联合化痰通络法高剂量组24h时相内对内皮细胞的保护作用最好,rt-PA联合化痰通络法中剂量组对3d和7d的内皮细胞形态保护为最佳.结论 rt-PA联合化痰通络法溶栓可以保护溶栓后血脑屏障完整性及海马缺血区微血管内皮细胞,针对溶栓后不同时相调整中药的用药浓度,可以起到更好的效果.
目的 觀察化痰通絡法對急性腦梗死大鼠重組組織型纖溶酶原激活物( rt-PA)溶栓後神經功能缺損評分、血腦屏障通透性及海馬內皮細胞超微結構的影響,為化痰通絡法防治急性腦梗死溶栓後齣血轉化提供實驗依據.方法 選擇健康雄性SD大鼠120隻分為6組,即假手術組、模型組、rt-PA組、低劑量中藥組、中劑量中藥組、高劑量中藥組(每組又分為6h、24h、3d、7d四箇時相),採用自身栓子法製備大腦中動脈栓塞(MCAO)模型大鼠,rt-PA組及中藥組分彆給予尾靜脈註入rt-PA及聯閤3種不同劑量化痰通絡中藥榦預.根據不同觀察時相對大鼠進行神經功能缺損評分(Bederson's評分),採用依文思藍染色(EB染色)檢測血腦屏障(BBB)通透性,併通過電鏡對大腦海馬缺血區內皮細胞超微結構進行觀察.結果 1.Bederson's評分:與模型組相比,rt-PA組、低劑量中藥組、中劑量中藥組、高劑量中藥組神經功能缺損評分明顯降低(P＜0.01)；與rt-PA組相比,以rt-PA聯閤化痰通絡法中劑量組、高劑量組神經功能缺損評分降低最為明顯(P＜0.05).2.EB染色:與模型組相比,rt-PA組、低劑量中藥組、中劑量中藥組、高劑量中藥組EB含量均明顯減低(P＜0.01)；與rt-PA組[6h:(236.72±79.79) ng/g；24h:(229.72±22.18) ng/g；3d:(163.34±24.45)ng/g；7d:( 133.21±25.09) ng/g]相比,以化痰通絡法聯閤rt-PA中劑量組[6h:(121.63±33.27) ng/g;24h:(153.78±60.67) ng/g;3d:(76.05±27.11 )ng/g;7d:(72.97±25.81) ng/g]、高劑量組[6h評分( 123.11±23.65) ng/g；24h評分(138.36±52.36) ng/g；3d評分(83.00±32.00) ng/g;7d評分(75.06±13.97) ng/g]EB含量降低最為明顯(P＜0.01).3.電鏡觀察:與模型組相比,治療組均起到保護內皮細胞的作用,其中以rt-PA聯閤化痰通絡法高劑量組24h時相內對內皮細胞的保護作用最好,rt-PA聯閤化痰通絡法中劑量組對3d和7d的內皮細胞形態保護為最佳.結論 rt-PA聯閤化痰通絡法溶栓可以保護溶栓後血腦屏障完整性及海馬缺血區微血管內皮細胞,針對溶栓後不同時相調整中藥的用藥濃度,可以起到更好的效果.
목적 관찰화담통락법대급성뇌경사대서중조조직형섬용매원격활물( rt-PA)용전후신경공능결손평분、혈뇌병장통투성급해마내피세포초미결구적영향,위화담통락법방치급성뇌경사용전후출혈전화제공실험의거.방법 선택건강웅성SD대서120지분위6조,즉가수술조、모형조、rt-PA조、저제량중약조、중제량중약조、고제량중약조(매조우분위6h、24h、3d、7d사개시상),채용자신전자법제비대뇌중동맥전새(MCAO)모형대서,rt-PA조급중약조분별급여미정맥주입rt-PA급연합3충불동제양화담통락중약간예.근거불동관찰시상대대서진행신경공능결손평분(Bederson's평분),채용의문사람염색(EB염색)검측혈뇌병장(BBB)통투성,병통과전경대대뇌해마결혈구내피세포초미결구진행관찰.결과 1.Bederson's평분:여모형조상비,rt-PA조、저제량중약조、중제량중약조、고제량중약조신경공능결손평분명현강저(P＜0.01)；여rt-PA조상비,이rt-PA연합화담통락법중제량조、고제량조신경공능결손평분강저최위명현(P＜0.05).2.EB염색:여모형조상비,rt-PA조、저제량중약조、중제량중약조、고제량중약조EB함량균명현감저(P＜0.01)；여rt-PA조[6h:(236.72±79.79) ng/g；24h:(229.72±22.18) ng/g；3d:(163.34±24.45)ng/g；7d:( 133.21±25.09) ng/g]상비,이화담통락법연합rt-PA중제량조[6h:(121.63±33.27) ng/g;24h:(153.78±60.67) ng/g;3d:(76.05±27.11 )ng/g;7d:(72.97±25.81) ng/g]、고제량조[6h평분( 123.11±23.65) ng/g；24h평분(138.36±52.36) ng/g；3d평분(83.00±32.00) ng/g;7d평분(75.06±13.97) ng/g]EB함량강저최위명현(P＜0.01).3.전경관찰:여모형조상비,치료조균기도보호내피세포적작용,기중이rt-PA연합화담통락법고제량조24h시상내대내피세포적보호작용최호,rt-PA연합화담통락법중제량조대3d화7d적내피세포형태보호위최가.결론 rt-PA연합화담통락법용전가이보호용전후혈뇌병장완정성급해마결혈구미혈관내피세포,침대용전후불동시상조정중약적용약농도,가이기도경호적효과.
Objective To observe the effects of Huatan Tongluo on the Bederson's scores,the permeability of blood-brain barrier and ultrastructure of hippocampus endothelial cells after thrombolysis in acute cerebral infarction.To provide experimental evidence for the prevention of bleeding after thrombolysis in acute cerebral infarction with Huatan Tongluo.Methods 120 SD male rats were randomly divided into sham group,model group,rt-PA group,Huatan Tongluo combined rt-PA low-dose group,Huatan Tougluo combined rt-PA medium-dose group and Huatan Tongluo combined rt-PA high-dose group( each group was divided in 6h,24h,3d,7d four-phase).MCAO rats were prepared by self-embolus method.rt-PA group and Huatan Tongluo combined rt-PA groups were given rt-PA through tail vein injection and three doses of Huatan Tongluo to treat.To observe the Bederson's scores in rat on four-phase.To detect the permeability of blood-brain barrier(BBB) by the Evans Blue dye ( EB dye) and watch the ultrastructure of hippocampus endothelial cells in ischemic region by electron microscopy.Results 1.Bederson's scores:Compared with model group,rt-PA group,Huatan Tongluo combined rt-PA low-dose group,Huatan Tongluo combined rt-PA medium-dose group and Huatan Tongluo combined rt-PA high-dose group significantly lower(P＜0.01 ) ; Compared with rt-PA group,Huatan Tongluo combined rt-PA medium-dose groups and high-dose groups was significantly lower(P ＜ 0.05).2.EB dye:Compared with model group,rt-PA group,Huatan Tongluo combined rt-PA low-dose group,Huatan Tongluo combined rt-PA medium-dose group and Huatan Tongluo combined rt-PA high-dose group (P ＜ 0.01 ) ; Compared with rt-PA group ( 6h:( 236.72 ± 79.79 ) ng/g; 24h:(229.72 ±22.18)ng/g;3d:(163.34 ±24.45)ng/g;7d:(133.21 ±25.09)ng/g),Huatan Tongluo combined rt-PA medium-dose groups (6h:( 121.63 ± 33.27) ng/g;24h:( 153.78 ± 60.67 ) ng/g;3d:( 76.05 ± 27.11 ) ng/g;7d:(72.97 ±25.81 ) ng/g) and high-dose groups (6h:( 123.11 ±23.65) ng/g;24h:( 138.36 ±52.36)ng/g;3d:(83.00 ±32.00)ng/g;7d:(75.06 ± 13.97) ng/g) was significantly lower(P＜ 0.01 ).3.Electron microscopy:Compared with model group,treatment groups all protected the endothelial cells.In 24h,the Huatan Tongluo combined rt-PA high-dose group was best.At 3d and 7d,the Huatan Tongluo combined rt-PA medium-dose group was best.Conclusion Huatan Tongluo combined rt-PA to thrombolysis can protect the blood-brain barrier and endothelial cells of hippocampal's ischemic region.After thrombolytic,to adjust the medicine drug concentration at different time can play a better treatment results.
