中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2009年
6期
555-558
,共4页
夏磊%殷铁林%杜琳%周若南%王义生
夏磊%慇鐵林%杜琳%週若南%王義生
하뢰%은철림%두림%주약남%왕의생
甲泼尼龙%脊髓缺血%再灌注损伤%热休克蛋白质类%肿瘤坏死因子-α
甲潑尼龍%脊髓缺血%再灌註損傷%熱休剋蛋白質類%腫瘤壞死因子-α
갑발니룡%척수결혈%재관주손상%열휴극단백질류%종류배사인자-α
Methylprednisolone%Spinal cord ischemia%Reperfusion injury%Heat-shock pro-teins%Tumor necrosis factor-α
目的 研究甲泼尼龙(MP)预处理脊髓缺血再灌注损伤(SCⅡ)对热休克蛋白27(HSP27)和肿瘤坏死因子α(TNF-α)的影响.方法 健康纯种成年雄性SD大鼠150只随机分成三组(n=50):对照组只暴露腹主动脉,不做模型;SCⅡ组夹闭腹土动脉30 min后松开,冉灌注3 h建立SCⅡ模型,然后处死大鼠,取腰段,脊髓标本;MP组于建立SCⅡ模型前30 min尾部静脉注射MP(30 mg/kg).通过HE染色观察损伤节段脊髓病删形态学变化,用免疫组织化学染色方法观察脊髓组织HSP27和TNF-α,的表达,并对大鼠后肢运动功能进行评分.结果 对照绀:神经元细胞轮廓清晰,极性存在,核圆,核仁清楚,尼氏体呈网状均匀排列于细胞核周围,胞浆均匀深染,组织结构完整清楚,无中性粒细胞浸润,无出血、水肿等异常现象;SCⅡ组:神经元细胞肿胀,数目减少,极性变钝,核仁轻度萎缩,尼氏体旱颗粒状,淡染,组织巾有中性粒细胞浸润;MP组:部分神经元细胞轻度水肿,极性稍变钝,核仁尚清,绀织中术见中性粒细胞浸润.SCⅡ组脊髓组织内HSP27及TNF-α大量表达,与对照绀对比差异有统计学意义(P<0.05).MP组脊髓细胞内TNF-α表达明显降低,而HSP27表达更显著, 者分别与SCⅡ组对比差异均有统计学意义(P<0.05).人鼠SCⅡ后肢功能评分降低,MP组大鼠后肢运动功能评分较SCⅡ组明显提高,差异有统计学意义(P<0.05).结论 MP预处理可降低大鼠SCⅡ中TNF-α的表达,增加HSP27的表达,并能提高大鼠后肢运动功能评分,预防性应用MP能减轻SCⅡ,保护脊髓神终功能.
目的 研究甲潑尼龍(MP)預處理脊髓缺血再灌註損傷(SCⅡ)對熱休剋蛋白27(HSP27)和腫瘤壞死因子α(TNF-α)的影響.方法 健康純種成年雄性SD大鼠150隻隨機分成三組(n=50):對照組隻暴露腹主動脈,不做模型;SCⅡ組夾閉腹土動脈30 min後鬆開,冉灌註3 h建立SCⅡ模型,然後處死大鼠,取腰段,脊髓標本;MP組于建立SCⅡ模型前30 min尾部靜脈註射MP(30 mg/kg).通過HE染色觀察損傷節段脊髓病刪形態學變化,用免疫組織化學染色方法觀察脊髓組織HSP27和TNF-α,的錶達,併對大鼠後肢運動功能進行評分.結果 對照紺:神經元細胞輪廓清晰,極性存在,覈圓,覈仁清楚,尼氏體呈網狀均勻排列于細胞覈週圍,胞漿均勻深染,組織結構完整清楚,無中性粒細胞浸潤,無齣血、水腫等異常現象;SCⅡ組:神經元細胞腫脹,數目減少,極性變鈍,覈仁輕度萎縮,尼氏體旱顆粒狀,淡染,組織巾有中性粒細胞浸潤;MP組:部分神經元細胞輕度水腫,極性稍變鈍,覈仁尚清,紺織中術見中性粒細胞浸潤.SCⅡ組脊髓組織內HSP27及TNF-α大量錶達,與對照紺對比差異有統計學意義(P<0.05).MP組脊髓細胞內TNF-α錶達明顯降低,而HSP27錶達更顯著, 者分彆與SCⅡ組對比差異均有統計學意義(P<0.05).人鼠SCⅡ後肢功能評分降低,MP組大鼠後肢運動功能評分較SCⅡ組明顯提高,差異有統計學意義(P<0.05).結論 MP預處理可降低大鼠SCⅡ中TNF-α的錶達,增加HSP27的錶達,併能提高大鼠後肢運動功能評分,預防性應用MP能減輕SCⅡ,保護脊髓神終功能.
목적 연구갑발니룡(MP)예처리척수결혈재관주손상(SCⅡ)대열휴극단백27(HSP27)화종류배사인자α(TNF-α)적영향.방법 건강순충성년웅성SD대서150지수궤분성삼조(n=50):대조조지폭로복주동맥,불주모형;SCⅡ조협폐복토동맥30 min후송개,염관주3 h건립SCⅡ모형,연후처사대서,취요단,척수표본;MP조우건립SCⅡ모형전30 min미부정맥주사MP(30 mg/kg).통과HE염색관찰손상절단척수병산형태학변화,용면역조직화학염색방법관찰척수조직HSP27화TNF-α,적표체,병대대서후지운동공능진행평분.결과 대조감:신경원세포륜곽청석,겁성존재,핵원,핵인청초,니씨체정망상균균배렬우세포핵주위,포장균균심염,조직결구완정청초,무중성립세포침윤,무출혈、수종등이상현상;SCⅡ조:신경원세포종창,수목감소,겁성변둔,핵인경도위축,니씨체한과립상,담염,조직건유중성립세포침윤;MP조:부분신경원세포경도수종,겁성초변둔,핵인상청,감직중술견중성립세포침윤.SCⅡ조척수조직내HSP27급TNF-α대량표체,여대조감대비차이유통계학의의(P<0.05).MP조척수세포내TNF-α표체명현강저,이HSP27표체경현저, 자분별여SCⅡ조대비차이균유통계학의의(P<0.05).인서SCⅡ후지공능평분강저,MP조대서후지운동공능평분교SCⅡ조명현제고,차이유통계학의의(P<0.05).결론 MP예처리가강저대서SCⅡ중TNF-α적표체,증가HSP27적표체,병능제고대서후지운동공능평분,예방성응용MP능감경SCⅡ,보호척수신종공능.
Objective To observe the effect of methylprednisolone (MP) pre-intervention on ex-pressions of heat shock protein 27 (HSP27) and tumor necrosis factor alpha (TNF-α) in cells in rat spinal cord following ischemia-reperfusion injury. Methods One hundred and fifty male Sprague-Dawley (SD) rats were randomly divided into 3 equal groups: group A (control) in which the abdominal aorta was exposed without any treatment, group B in which the abdominal aorta was clipped for 30 minutes before reperfusion for 3 bours to establish a model of ischemia- reperfusion injury, and group C in which intravenous MP injection was conducted 30 minutes before the establishment of the ischemia-reperfusion injury model. Three hours later the spinal cords were harvested. Pathological changes of spinal cord cells were observed with HE staining and expressions of HSP27 and TNF-α in spinal cord cells were observed with immunohistochemical staining. The motor function of hind-limbs before was evaluated before sample harvest. The data were analyzed with SPSS software. Results There were significant differences between groups A and B in the expressions of TNF-α and HSP27. Compared with group B, the expression of TNF-α decreased and HSP27 increased in group C, with statistically significant differences between the 2 groups. The motor function score of hind-limbs decreased in group B but improved in group C. Conclusions Since MP can decrease the expression of TNF-α and up-regulate the expression of HSP27, it has a potency of neuro-protection. Spinal cord ischemia-reperfusion injury can be avoided or decreased after MP pre-intervention.
