CAJ | 학술논문

目的建立离体眼球表面灌流系统(isolated eye superfusion system,IESS),评价IESS对离体眼球正常形态和功能的维持能力.方法在文献报道基础上,应用仿生学原理,结合具体实验条件,自行设计制作离体眼球表面灌流系统.选取8只经检测未发生损伤的离体兔眼置于IESS中,在不同时间点对离体眼球进行角膜混浊度、荧光素滞留度、角膜水肿率和病理组织学改变进行检测,从而评价IESS的功能.以上实验间隔1周进行1次,共进行3次.结果成功设计制作了具有自主知识产权的IESS.3组评价试验中,离体眼球角膜混浊度评分分别为0.00±0.00、0.01±0.03和0.00±0.01;荧光素滞留度评分分别为0.03±0.09、0.00±0.00和0.03±0.09;角膜水肿率分别为(2.06±1.63)%、(2.83±1.93)%和(2.03±1.19)%,以上指标均在允许范围内,并未见明显的角膜病理组织学损伤.IESS工作稳定性良好,3组实验间各指标差异无统计学意义(P＞ 0.05).结论 IESS可以维持离体眼球在实验期间的正常形态,且具有较高的稳定性和可靠性,可以用于试验用离体眼球的短期保存.
목적 건립리체안구표면관류계통(isolated eye superfusion system,IESS),평개IESS대리체안구정상형태화공능적유지능력.방법 재문헌보도기출상,응용방생학원리,결합구체실험조건,자행설계제작리체안구표면관류계통.선취8지경검측미발생손상적리체토안치우IESS중,재불동시간점대리체안구진행각막혼탁도、형광소체류도、각막수종솔화병리조직학개변진행검측,종이평개IESS적공능.이상실험간격1주진행1차,공진행3차.결과 성공설계제작료구유자주지식산권적IESS.3조평개시험중,리체안구각막혼탁도평분분별위0.00±0.00、0.01±0.03화0.00±0.01;형광소체류도평분분별위0.03±0.09、0.00±0.00화0.03±0.09;각막수종솔분별위(2.06±1.63)%、(2.83±1.93)%화(2.03±1.19)%,이상지표균재윤허범위내,병미견명현적각막병리조직학손상.IESS공작은정성량호,3조실험간각지표차이무통계학의의(P＞ 0.05).결론 IESS가이유지리체안구재실험기간적정상형태,차구유교고적은정성화가고성,가이용우시험용리체안구적단기보존.
Objective To establish the isolated eye superfusion system (IESS) and evaluate its maintenance for normal appearance and functions of isolated eyes. Methods According to bionics principles, a home-made IESS was developed with reference to literatures and adapted to special condition of our experiment. Eight healthy isolated rabbit eyes were selected to be placed in the IESS and studied for the opacity, fluorescein retention, edema and histopathology over time so as to evaluate the capacities of IESS.These measurements were performed every two weeks for 3 times. Results A patented IESS was successfully designed and manufactured. Three sessions of evaluation did not identify any histopathological changes in the cornea, as reflected by the within-normal measurements for corneal opacity scores (0.00±0.00, 0.01±0.03 and 0.00±0.01), fluorescein retention scores (0.03±0.09, 0.00±0.00 and 0.03±0.09), and rates of corneal edema [(2.06±1.63)%, (2.83±1.93)% and (2.03± 1.19)% ].The IESS showed good functional stability, and there were no significant ditferences among the three sessions of measurement for each parameter (P＞0.05). Conclusions IESS can maintain the normal appearance and function of the isolated eyes throughout the whole experimental periods. The high stability and reliability of IESS may support its use for short-term storage of experimental isolated eyes.