中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
4期
489-492
,共4页
张黎龙%田融%张学利%夏刚%申庆丰%刘岩%于斌
張黎龍%田融%張學利%夏剛%申慶豐%劉巖%于斌
장려룡%전융%장학리%하강%신경봉%류암%우빈
骨移植%富血小板血浆%腰椎%脊柱融合术
骨移植%富血小闆血漿%腰椎%脊柱融閤術
골이식%부혈소판혈장%요추%척주융합술
Platelet- rich plasma%Lumbar vertebra%Bone transplantation%Spinal fusion
目的 研究富血小板血浆(PRP)在家兔腰椎后外侧植骨融合中的作用,为脊柱的融合找到一个新的途径.方法 制备PRP,取家兔24只,取双侧肋骨,行双侧第5、6腰椎横突间的后外侧植骨融合.采用自身对照方法,实验侧取PRP复合肋骨骨粒植入融合区,对照侧取肋骨骨粒植入融合区.术后4、8、12周处死各处死8只动物取脊柱标本.利用影像学、组织学的手段观察植骨区的融合情况,从大体、组织、细胞等方面分析植骨融合情况.结果 PRP复合自体骨组术后4、8、12周各项评分明显优于单纯自体骨组(P＜0.05).实验侧植骨区阻射密度值4、8和12周分别为51±7、69±7、81 ±6；对照侧为33 ±9、40 ±6、50 ±6,2组同时间点比较差异有统计学意义(均P＜0.05).实验侧植骨区放射学评分4、8和12周分别为(3.1±0.8)、(6.9±1.1)、(8.1±1.1)分,2组同时间点比较差异有统计学意义(P均＜0.05).结论 PRP复合自体骨移植对家兔腰椎后外侧植骨融合具有促进作用.
目的 研究富血小闆血漿(PRP)在傢兔腰椎後外側植骨融閤中的作用,為脊柱的融閤找到一箇新的途徑.方法 製備PRP,取傢兔24隻,取雙側肋骨,行雙側第5、6腰椎橫突間的後外側植骨融閤.採用自身對照方法,實驗側取PRP複閤肋骨骨粒植入融閤區,對照側取肋骨骨粒植入融閤區.術後4、8、12週處死各處死8隻動物取脊柱標本.利用影像學、組織學的手段觀察植骨區的融閤情況,從大體、組織、細胞等方麵分析植骨融閤情況.結果 PRP複閤自體骨組術後4、8、12週各項評分明顯優于單純自體骨組(P＜0.05).實驗側植骨區阻射密度值4、8和12週分彆為51±7、69±7、81 ±6；對照側為33 ±9、40 ±6、50 ±6,2組同時間點比較差異有統計學意義(均P＜0.05).實驗側植骨區放射學評分4、8和12週分彆為(3.1±0.8)、(6.9±1.1)、(8.1±1.1)分,2組同時間點比較差異有統計學意義(P均＜0.05).結論 PRP複閤自體骨移植對傢兔腰椎後外側植骨融閤具有促進作用.
목적 연구부혈소판혈장(PRP)재가토요추후외측식골융합중적작용,위척주적융합조도일개신적도경.방법 제비PRP,취가토24지,취쌍측륵골,행쌍측제5、6요추횡돌간적후외측식골융합.채용자신대조방법,실험측취PRP복합륵골골립식입융합구,대조측취륵골골립식입융합구.술후4、8、12주처사각처사8지동물취척주표본.이용영상학、조직학적수단관찰식골구적융합정황,종대체、조직、세포등방면분석식골융합정황.결과 PRP복합자체골조술후4、8、12주각항평분명현우우단순자체골조(P＜0.05).실험측식골구조사밀도치4、8화12주분별위51±7、69±7、81 ±6；대조측위33 ±9、40 ±6、50 ±6,2조동시간점비교차이유통계학의의(균P＜0.05).실험측식골구방사학평분4、8화12주분별위(3.1±0.8)、(6.9±1.1)、(8.1±1.1)분,2조동시간점비교차이유통계학의의(P균＜0.05).결론 PRP복합자체골이식대가토요추후외측식골융합구유촉진작용.
Objective To study the effect of platelet-rich plasma (PRP) in the posterolateral fusion of rabbit lumbar and to provide the new approach for the fusion of spine.Methods PRP was prepared,bilateral ribs were taken from 24 rabbits and lumbar 5 and 6 underwent bilateral posterolateral intertransverse fusion.Using selfcontrol method,the bone particles with PRP was implanted to fusion zone in the experimental side,and the bone particles in the control side.Rabbits were sacrificed after 4,8 and 12 weeks to take the spine specimens.The spine fusion were observed by means of histology and imaging to evaluate the situation of fusion from the general,tissues,cells and other aspects.Results 4,8 and 12 weeks after surgery,the score was significantly better in experimental group than that in control group.The radiopacity density of fusion bone were 51 ±7,69 ± 7,81 ± 6 in experimental group and 33 ±9,40 ±6,50 ±6 in control group at 4,8 and 12 weeks after surgery respectively.There was significant difference on the density at any different time between these two groups(P ＜ 0.05 ).Radiology score of fusion bone were ( 3.1 ± 0.8 ),(6.9 ± 1.1 ),( 8.1 ± 1.1 ) in experimental group at 4,8 and 12 weeks after surgery respectively,and there was also significant difference on the score at any different time between these two groups(P ＜0.05).Conclusion The autogenous bone with PRP can promote the posterolateral fusion of rabbit lumbar.
