中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2011年
11期
1115-1118
,共4页
朱永华%杨有业%林少华%漆松涛%黄汉添%廖巍%胡子慧%李亮明%林其昌%罗勇
硃永華%楊有業%林少華%漆鬆濤%黃漢添%廖巍%鬍子慧%李亮明%林其昌%囉勇
주영화%양유업%림소화%칠송도%황한첨%료외%호자혜%리량명%림기창%라용
颅内动脉瘤%afamin%血清蛋白%双向差异凝胶电泳
顱內動脈瘤%afamin%血清蛋白%雙嚮差異凝膠電泳
로내동맥류%afamin%혈청단백%쌍향차이응효전영
Intracranial aneurysm%Afamin%Serum protein%Two-dimensional differential in-gel electrophoresis
目的 寻找未破裂颅内动脉瘤患者较正常成人血清中表达差异的蛋白.方法 选择中山大学附属中山医院(中山市人民医院)神经外科自2007年7月至2009年9月收治的未破裂颅内动脉瘤患者8例,同期体检健康者8例作为正常对照组,应用双向差异凝胶电泳(2-D DIGE)、基质辅助激光解析离子化飞行时间质谱(MALDI-TOF-MS)分析并鉴定2组血清中差异蛋白质的表达.结果 与对照组比较,未破裂颅内动脉瘤患者血清中有29个表达差异在1.5倍以上的蛋白点,其中579蛋白点在未破裂颅内动脉瘤患者血清中比对照组表达降低1.81倍,差异有统计学意义(P=0.008),经MALDI-TOF-MS鉴定为afamin.结论 afamin在未破裂颅内动脉瘤患者血清中的表达较低,可能参与了颅内动脉瘤的形成及扩张.
目的 尋找未破裂顱內動脈瘤患者較正常成人血清中錶達差異的蛋白.方法 選擇中山大學附屬中山醫院(中山市人民醫院)神經外科自2007年7月至2009年9月收治的未破裂顱內動脈瘤患者8例,同期體檢健康者8例作為正常對照組,應用雙嚮差異凝膠電泳(2-D DIGE)、基質輔助激光解析離子化飛行時間質譜(MALDI-TOF-MS)分析併鑒定2組血清中差異蛋白質的錶達.結果 與對照組比較,未破裂顱內動脈瘤患者血清中有29箇錶達差異在1.5倍以上的蛋白點,其中579蛋白點在未破裂顱內動脈瘤患者血清中比對照組錶達降低1.81倍,差異有統計學意義(P=0.008),經MALDI-TOF-MS鑒定為afamin.結論 afamin在未破裂顱內動脈瘤患者血清中的錶達較低,可能參與瞭顱內動脈瘤的形成及擴張.
목적 심조미파렬로내동맥류환자교정상성인혈청중표체차이적단백.방법 선택중산대학부속중산의원(중산시인민의원)신경외과자2007년7월지2009년9월수치적미파렬로내동맥류환자8례,동기체검건강자8례작위정상대조조,응용쌍향차이응효전영(2-D DIGE)、기질보조격광해석리자화비행시간질보(MALDI-TOF-MS)분석병감정2조혈청중차이단백질적표체.결과 여대조조비교,미파렬로내동맥류환자혈청중유29개표체차이재1.5배이상적단백점,기중579단백점재미파렬로내동맥류환자혈청중비대조조표체강저1.81배,차이유통계학의의(P=0.008),경MALDI-TOF-MS감정위afamin.결론 afamin재미파렬로내동맥류환자혈청중적표체교저,가능삼여료로내동맥류적형성급확장.
Objective To search the differenially expressed proteins in the serum of patients with unruptured intracranial aneurysm.Methods Eight patients with unruptured intracranial aneurysm,admitted to our hospital from July 2007 to September 2009,and 8 healthy adults(controls)were recruited in our study; two-dimensional differential in-gel electrophoresis(2D DIGE)and matrix-assisted laser desorption/ionization-time of flight mass spectrometry(MALDI-TOF-MS)were employed to analyze and identify the differentially expressed proteins in the serum of these patients and controls.Results As compared with the control group,patients with unruptured intracranial aneurysm had 29 differentially expressed proteins those enjoyed more than 1.5 folds differences; protein at spot 579was expressed as low as 1.81 folds in the serum of patients with unruptured intracranial aneurysm as compared with that in controls(P=0.008); the protein was identified as afamin using MALDI-TOF-MS.Conclusion The low expression of afamin in patients with unruptured intracranial aneurysm probably contributes to the formation and expansion of intracranial aneurysm.
