CAJ | 학술논문

目的研究三七总皂苷(PNS)对急性心肌梗死(AMI)后左室重构(LVRM)大鼠血清肿瘤坏死因子-α(TNF-α)与基质金属蛋白酶-2(MMP-2)表达的影响并探讨其作用机制.方法除假手术组外采用结扎大鼠左冠状动脉前降支的方法建立AMI模型,术后24h后随机分为对照组和实验组,连续4周分别灌胃给予生理盐水、福辛普利和PNS低、中、高剂量,观察PNS对病鼠左室舒张末期内径(宽度)(LVIDd)、左室收缩末期内径(宽度)(LVIDs)、左室射血分数(EF)、左室收缩百分率(FS)、二尖瓣口舒张早期血流速度(MY)、心率等反映心功能变化指标及TNF-α与MMP-2表达的影响.结果 PNS中、高剂量组的EF、FS及MV指标均显著提高(P<0.01或0.05);PNS低、中、高剂量组均能明显抑制TNF-α与MMP-2的表达(P<0.05).结论 PNS能够抑制TNF-α与MMP-2的分泌与释放,通过提高左室收缩和舒张功能,降低外周阻力,减轻AMI后LVRM大鼠的心肌缺血再灌注损伤,对病鼠AMI后LVRM心功能有保护和改善作用.
목적 연구삼칠총조감(PNS)대급성심기경사(AMI)후좌실중구(LVRM)대서혈청종류배사인자-α(TNF-α)여기질금속단백매-2(MMP-2)표체적영향병탐토기작용궤제.방법 제가수술조외채용결찰대서좌관상동맥전강지적방법건립AMI모형,술후24h후수궤분위대조조화실험조,련속4주분별관위급여생리염수、복신보리화PNS저、중、고제량,관찰PNS대병서좌실서장말기내경(관도)(LVIDd)、좌실수축말기내경(관도)(LVIDs)、좌실사혈분수(EF)、좌실수축백분솔(FS)、이첨판구서장조기혈류속도(MY)、심솔등반영심공능변화지표급TNF-α여MMP-2표체적영향.결과 PNS중、고제량조적EF、FS급MV지표균현저제고(P<0.01혹0.05);PNS저、중、고제량조균능명현억제TNF-α여MMP-2적표체(P<0.05).결론 PNS능구억제TNF-α여MMP-2적분비여석방,통과제고좌실수축화서장공능,강저외주조력,감경AMI후LVRM대서적심기결혈재관주손상,대병서AMI후LVRM심공능유보호화개선작용.
Objective To observe the effects of panax notoginsenoside (PNS) on tumor necrosis factor-α (TNF-α) and matrix metalloproteinases-2 (MMP-2) expressions in rats with post-myocardial infarction ventricular remodeling and explore the mechanism. Methods Rat models of acute infarction ventricular (AMI) were established by ligation of the left anterior descending coronary artery. Twenty-four hours after the operation, the rats were randomized into control and experimental groups for intragastric administration of normal saline (control), fosinopril and PNS at the low, medium and high doses for 4 consecutive weeks. The effects of PNS on the cardiac function index including the left ventricular end-diastolic dimension (LVIDd), left ventricular end-systolic diameter (LVIDs), ejection fraction (EF), percentage of left ventricular systole (FS), mitral early diastolic flow velocity mouth (MV), and heart rate (HR) were observed, and the changes in TNF-α and MMP-2 expression were detected after post-myocardial infarction ventricular remodeling. Results Compared with the control group, PNS at the medium and high doses produced significant improvements in the EF, FS and MV of the rats (P<0.01 or 0.05). TNF-α and MMP-2 expressions were significantly decreased by PNS treatment at low, medium and high doses (P<0.01). Conclusion PNS can inhibit or reduce the expression of TNF-α and MMP-2, thereby enhancing left ventricular systolic and diastolic functions, decreasing peripheral resistance, and improving the cardiac function of rats with post-myocardial infarction left ventricular remodeling.