CAJ | 학술논문

目的检测分析牙周组织局部滴注硝酸盐后,一氧化氮(NO)含量变化与局部炎症发生和发展的关系,初步探讨硝酸盐在牙周炎症预防与治疗中的作用.方法建立大鼠实验性牙周炎动物模型,将70只健康SD大鼠随机分为正常对照组(N组),牙龈炎组(P组)和药物对照组(PP组),其中牙龈炎组又分为1000uM/L硝酸钾(KNO_3)滴注组和非KNO3滴注组,所有动物分别于术后1、4周处死,采用酶法检测实验动物牙龈组织中NO_2~-和NO_3~-的含量,并用肉眼和组织切片观察牙龈组织中炎症的变化情况.结果正常对照组与牙龈炎组中未滴注1000uM/L KNO3溶液的牙龈组织中NO含量有高度显著性差异(P＜0.01),在牙龈炎组中,牙龈局部给予KNO_3溶液滴注与未给予KNO_3溶液滴注的牙龈组织中NO含量无显著性差异(P＞0.01),药物对照组与正常对照组牙龈组织中NO含量有高度显著性差异(P＜0.01),药物对照组(PP组)与P组未滴注KNO_3溶液的牙龈组织中NO含量有显著性差异(P＜0.01).结论牙周局部给予硝酸盐滴注后能够增加NO的产生,提高局部NO的浓度,从而改善微循环,增强牙周局部抵抗能力,减轻和预防牙周炎症的发生.
목적 검측분석아주조직국부적주초산염후,일양화담(NO)함량변화여국부염증발생화발전적관계,초보탐토초산염재아주염증예방여치료중적작용.방법 건립대서실험성아주염동물모형,장70지건강SD대서수궤분위정상대조조(N조),아간염조(P조)화약물대조조(PP조),기중아간염조우분위1000uM/L초산갑(KNO_3)적주조화비KNO3적주조,소유동물분별우술후1、4주처사,채용매법검측실험동물아간조직중NO_2~-화NO_3~-적함량,병용육안화조직절편관찰아간조직중염증적변화정황.결과 정상대조조여아간염조중미적주1000uM/L KNO3용액적아간조직중NO함량유고도현저성차이(P＜0.01),재아간염조중,아간국부급여KNO_3용액적주여미급여KNO_3용액적주적아간조직중NO함량무현저성차이(P＞0.01),약물대조조여정상대조조아간조직중NO함량유고도현저성차이(P＜0.01),약물대조조(PP조)여P조미적주KNO_3용액적아간조직중NO함량유현저성차이(P＜0.01).결론 아주국부급여초산염적주후능구증가NO적산생,제고국부NO적농도,종이개선미순배,증강아주국부저항능력,감경화예방아주염증적발생.
Objective To investigate the effects of nitrate on the treatment of periodontitis by analyzing the relationship between nitric oxide (NO) in gingival tissue and local inflammation after nitrate was used in the periodontal tissue of normal and gingivitis.Methods A periodontal animal model was established on SD rats. Seventy SD rats were randomly assigned to the normal group, gingivitis group and drug control group. In gingivitis group, two sub-groups were also assigned with nitrate application and non-application. The animals were sacrificed at 1and 4 weeks later. NO_2~- and NO_3~- level in gingival tissue was measured by NO kit, the change of inflammation in periodontal tissue was observed visually and histologically.Results There was highly significant difference of NO between normal group and the gingival tissue which nitrate was not used in gingivitis group (P＜0.01). Among gingivitis group, there was no significant difference in NO level between gingival tissue which nitrate was used and was not used (P＞0.01). The highly significant differences of NO level were found between drug control group and normal group (P＜0.01). There was also significant difference of NO level in gingival tissue between drug control group and gingivitis group in 1 week and 4 weeks (P＜0.01).Conclusion The microcirculation and the ability of anti-infection of periodontal tissue could be improved by nitrate. Therefore, the degree of periodontitis could be relieved.