CAJ | 학술논문

目的研究脱氧核糖核酸酶Ⅰ(DNase-Ⅰ)纯化结合碱性裂解法提取混合斑精子DNA的方法在法医学中的应用.方法收集79份性犯罪案件混合斑检材,分别用DNase-Ⅰ纯化结合碱性裂解法和差异裂解法提取精子DNA,采用STR荧光标记复合扩增体系进行16个STR基因座分型,并比较检验结果.结果应用DNase-Ⅰ纯化结合碱性裂解法提取精子DNA,64例检材分型成功;应用差异裂解法提取精子DNA,57例检材分型成功;两种方法比较结果存在显著性差异(P=0.039),DNase-1纯化结合碱性裂解法提取精子DNA的STR分型成功率更高,成本低廉.结论 DNase-Ⅰ纯化结合碱性裂解法提取混合斑精子DNA 可提高检验成功率,操作简便,快速,易于自动化,适于法医学个体识别鉴定.
목적 연구탈양핵당핵산매Ⅰ(DNase-Ⅰ)순화결합감성렬해법제취혼합반정자DNA적방법재법의학중적응용.방법 수집79빈성범죄안건혼합반검재,분별용DNase-Ⅰ순화결합감성렬해법화차이렬해법제취정자DNA,채용STR형광표기복합확증체계진행16개STR기인좌분형,병비교검험결과.결과 응용DNase-Ⅰ순화결합감성렬해법제취정자DNA,64례검재분형성공;응용차이렬해법제취정자DNA,57례검재분형성공;량충방법비교결과존재현저성차이(P=0.039),DNase-1순화결합감성렬해법제취정자DNA적STR분형성공솔경고,성본저렴.결론 DNase-Ⅰ순화결합감성렬해법제취혼합반정자DNA 가제고검험성공솔,조작간편,쾌속,역우자동화,괄우법의학개체식별감정.
Objective To establish a method of sperm DNA extraction in mixed stain by using DNase-Ⅰ purificationcombined with alkaline lysis method in forensic science.Methods 79 mixed stain samples of criminal cases were collected.Sperm DNA was extracted using the purification of DNase-Ⅰ binding alkaline lysis method.16 STR loci were genotyped with fluorescent multiplex amplification system.The typing results were compared with that of extracted using two-step differential extraction procedure.Results Of all 79 mixed stain samples,64 samples were genotyped successfully by using DNase-Ⅰ purification combined with alkaline lysis method while 57 samples were genotyped successfully with two-step differential extraction procedure.There was significant difference between two methods(P=0.039).The purification of DNase-Ⅰ binding alkaline lysis method had a higher success rate and lower cost than that of two-step differential extraction procedure.Conclusion Purification of DNase-Ⅰ binding alkaline lysis method can increase the typing success rate of the mixed stain samples.The method is simple,rapid and easy to be automated,and suitable for forensic identification test.