CAJ | 학술논문

目的:探讨吲哚胺吡咯2,3-双加氧酶(IDO,一种具有免疫调节作用的酶)在肾细胞癌(RCC)患者中的作用.方法:在中南大学湘雅二医院诊断为RCC的患者40例,全部接受肾切除术.肾组织的免疫病理检查采用半定量方法评估.实时定量PCR(RT-qPCR)检测RCC与非肾癌肾组织IDO的mRNA水平.免疫组织化学的方法检测血管内皮细胞的IDO蛋白表达.同时依据IDO的mRNA水平计算出患者的Kaplan-Meier生存曲线.结果:RCC肿瘤组织中IDO mRNA的表达明显高于正常肾组织(P＜0.001).在RCC患者中,IDO高表达的患者比那些低IDO表达的患者具有明显较长的生存时间(P=0.01).统计学分析显示:RCC肿瘤组织中IDO的水平和肿瘤增殖标志物Ki67之间呈负相关,IDO水平高的患者Ki67水平低,反之亦然(P＜0.01).结论:IDO可能作为预测RCC患者预后的生物标志物.
목적:탐토신타알필각2,3-쌍가양매(IDO,일충구유면역조절작용적매)재신세포암(RCC)환자중적작용.방법:재중남대학상아이의원진단위RCC적환자40례,전부접수신절제술.신조직적면역병리검사채용반정량방법평고.실시정량PCR(RT-qPCR)검측RCC여비신암신조직IDO적mRNA수평.면역조직화학적방법검측혈관내피세포적IDO단백표체.동시의거IDO적mRNA수평계산출환자적Kaplan-Meier생존곡선.결과:RCC종류조직중IDO mRNA적표체명현고우정상신조직(P＜0.001).재RCC환자중,IDO고표체적환자비나사저IDO표체적환자구유명현교장적생존시간(P=0.01).통계학분석현시:RCC종류조직중IDO적수평화종류증식표지물Ki67지간정부상관,IDO수평고적환자Ki67수평저,반지역연(P＜0.01).결론:IDO가능작위예측RCC환자예후적생물표지물.
Objective:To explore the role of indoleamine-pyrrole 2,3-dioxygenase (IDO),an immunomodulatory enzyme,in renal cell carcinoma (RCC).Methods:A total of 40 patients diagnosed as RCC in the Second Xiangya Hospital were included in this study.All patients received nephrectomy.The histopathological features of samples were assessed semi-quantitatively.IDO mRNA level in RCC and non-RCC renal tissues was determined by real-time quantitative PCR (RT-qPCR).And the expression of IDO protein in endothelial cells was examined by immunohistochemistry; a Kaplan-Meier survival curves was calculated on the basis of IDO mRNA level.Results:Level of IDO mRNA in RCC samples was significantly higher than that in tumor-free samples with P＜0.001.Patients with high IDO expression had an significantly longer survival time than those with low IDO expression (P=0.01).There was a statistically significant inverse correlation between IDO and proliferation marker Ki67.Patients with high IDO level were of low Ki67 level,and vice versa (P＜0.01).Conclusion:IDO might be a prognostic biomarker for patients with RCC.