CAJ | 학술논문

目的探究复方苦参注射液对人肝癌SMMC-7721细胞凋亡的影响.方法无菌培养人肝癌细胞SMMC-7721,采用MTT法检测复方苦参注射液对SMMC7721细胞的影响,复方苦参注射液的浓度分别为3.34、2.38、1.43和0.48 mg/ml;采用膜联蛋白V-异硫氰酸荧光素(Annexin V-FITC)/碘化丙啶(propidium iodide,PI)双染法测肝癌细胞凋亡率,复方苦参注射液的浓度分别为0.86、1.72和3.43 mg/ml.结果复方苦参注射液浓度3.34、2.38、1.43、0.48 mg/ml作用于SMMC-7721 24 h的细胞抑制率分别为51.03％、53.67％、49.83％、0.03％；作用48 h后,上述浓度的细胞抑制率分别为67.14％、65.35％、62.25％、0.05％,作用72 h后,分别为74.16％、68.77％、66.04％、26.02％.结果复方苦参注射液浓度0.86、1.72和3.43 mg/ml作用于SMMC-7721 24 h的细胞凋亡率分别为(2.86±0.35)％、(15.16±1.15)％、(13.17±0.40)％；作用48 h后,上述浓度的细胞凋亡率分别为(8.57±0.44)％、(28.07±0.76)％、(29.81±8.10)％,均高于空白对照组(1.05±0.09)％(P＜0.05).结论复方苦参注射液对人肝癌SMMC-7721细胞增殖具有抑制作用,其作用机制与诱导细胞凋亡有关.
목적 탐구복방고삼주사액대인간암SMMC-7721세포조망적영향.방법 무균배양인간암세포SMMC-7721,채용MTT법검측복방고삼주사액대SMMC7721세포적영향,복방고삼주사액적농도분별위3.34、2.38、1.43화0.48 mg/ml;채용막련단백V-이류청산형광소(Annexin V-FITC)/전화병정(propidium iodide,PI)쌍염법측간암세포조망솔,복방고삼주사액적농도분별위0.86、1.72화3.43 mg/ml.결과 복방고삼주사액농도3.34、2.38、1.43、0.48 mg/ml작용우SMMC-7721 24 h적세포억제솔분별위51.03％、53.67％、49.83％、0.03％；작용48 h후,상술농도적세포억제솔분별위67.14％、65.35％、62.25％、0.05％,작용72 h후,분별위74.16％、68.77％、66.04％、26.02％.결과 복방고삼주사액농도0.86、1.72화3.43 mg/ml작용우SMMC-7721 24 h적세포조망솔분별위(2.86±0.35)％、(15.16±1.15)％、(13.17±0.40)％；작용48 h후,상술농도적세포조망솔분별위(8.57±0.44)％、(28.07±0.76)％、(29.81±8.10)％,균고우공백대조조(1.05±0.09)％(P＜0.05).결론 복방고삼주사액대인간암SMMC-7721세포증식구유억제작용,기작용궤제여유도세포조망유관.
Objective To explore the compound matrine injection on human hepatoma smmc-7721 cells' proliferation.Methods Human hepatoma smmc-7721 cells were sterilely cultured.Assayed the effects of compound matrine injection on smmc-7721 cells by MTT.The concentrations of the compound matrine injection were 3.34,2.38,1.43 and 0.48 mg/ml; Used the double staining method of annexin V-fluorescein isothiocyanate(Annexin V-FITC)/propidium iodide (propidium iodide,PI) to measure the apoptosis of hepatoma cell.The concentrations of the compound matrine injection were 0.86,1.72,and 3.43 mg/ml.Results The apoptosis rates of compound matrine injection with different concentration of 3.34,2.38,1.43 and 0.48 mg/ml on liver cancer cell SMMC-7721 were51.03％、53.67％、49.83％ and 0.03％ respectively after 24 hours; and 67.14％、65.35％、62.25％ and 0.05％ respectively after 48 hours;and 74.16％、68.77％、66.04％ and 26.02％respectively after 72 hours.The apoptosis rates of compound matrine injection with different concentration of 0.86,1.72 and 3.43 mg/ml on liver cancer cell SMMC-7721 were(2.86±0.35)％,(15.16± 1.15)％and(13.17±0.40)％ respectively after 24 hours; and (8.57±0.44)％,(28.07±0.76)％ and (29.81±8.10)％respectively after 48 hours,which were significantly higher than the control group (1.05±0.09)％(P＜0.05).Conclusion The compound matrine injection has a significant inhibition on human hepatoma smmc-7721 cells' proliferation and the mechanism was its inducing cell apoptosis.