中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2008年
3期
173-175
,共3页
李清华%徐萍%陈令全%刘丕%吕农华
李清華%徐萍%陳令全%劉丕%呂農華
리청화%서평%진령전%류비%려농화
胰腺炎,性坏死性%噻唑烷二酮类%模型,动物
胰腺炎,性壞死性%噻唑烷二酮類%模型,動物
이선염,성배사성%새서완이동류%모형,동물
Pancreatitis,acute necrotizing%Thiazolidinediones%Models,animal
目的 探讨吡格列酮预处理对ANP大鼠的影响.方法 54只大鼠采用经胆胰管逆行注射5%牛磺胆酸钠制备ANP模型.大鼠按随机数字法分为ANP组、吡格列酮组和假手术组,各18只.吡格列酮组在制模前2 h腹腔注射0.2%吡格列酮20 mg/kg体重.分别在术后3 h、6 h、12 h处死动物,取血检测血淀粉酶,取胰腺组织观察胰腺大体及组织学变化,分别按Hushes和Kusske标准评分.结果 术后3 h、6 h、12 h,ANP组及吡格列酮组血淀粉酶、胰腺大体病理的Hughes评分和胰腺组织学Kusske评分比假手术组明显升高;吡格列酮组大鼠术后12 h血淀粉酶、胰腺Hughes评分和Kusske评分分别为(2 980±1 080)U/L、4.50±2.07和7.50±1.05,均明显低于ANP组的(7 598±1 072)U/L、7.17±1.47和11.33±1.75(P＜0.01).结论 吡格列酮预处理可降低ANP大鼠血清淀粉酶,减轻胰腺大体、组织学病理改变,说明吡格列酮具有预防ANP的效应.
目的 探討吡格列酮預處理對ANP大鼠的影響.方法 54隻大鼠採用經膽胰管逆行註射5%牛磺膽痠鈉製備ANP模型.大鼠按隨機數字法分為ANP組、吡格列酮組和假手術組,各18隻.吡格列酮組在製模前2 h腹腔註射0.2%吡格列酮20 mg/kg體重.分彆在術後3 h、6 h、12 h處死動物,取血檢測血澱粉酶,取胰腺組織觀察胰腺大體及組織學變化,分彆按Hushes和Kusske標準評分.結果 術後3 h、6 h、12 h,ANP組及吡格列酮組血澱粉酶、胰腺大體病理的Hughes評分和胰腺組織學Kusske評分比假手術組明顯升高;吡格列酮組大鼠術後12 h血澱粉酶、胰腺Hughes評分和Kusske評分分彆為(2 980±1 080)U/L、4.50±2.07和7.50±1.05,均明顯低于ANP組的(7 598±1 072)U/L、7.17±1.47和11.33±1.75(P＜0.01).結論 吡格列酮預處理可降低ANP大鼠血清澱粉酶,減輕胰腺大體、組織學病理改變,說明吡格列酮具有預防ANP的效應.
목적 탐토필격렬동예처리대ANP대서적영향.방법 54지대서채용경담이관역행주사5%우광담산납제비ANP모형.대서안수궤수자법분위ANP조、필격렬동조화가수술조,각18지.필격렬동조재제모전2 h복강주사0.2%필격렬동20 mg/kg체중.분별재술후3 h、6 h、12 h처사동물,취혈검측혈정분매,취이선조직관찰이선대체급조직학변화,분별안Hushes화Kusske표준평분.결과 술후3 h、6 h、12 h,ANP조급필격렬동조혈정분매、이선대체병리적Hughes평분화이선조직학Kusske평분비가수술조명현승고;필격렬동조대서술후12 h혈정분매、이선Hughes평분화Kusske평분분별위(2 980±1 080)U/L、4.50±2.07화7.50±1.05,균명현저우ANP조적(7 598±1 072)U/L、7.17±1.47화11.33±1.75(P＜0.01).결론 필격렬동예처리가강저ANP대서혈청정분매,감경이선대체、조직학병리개변,설명필격렬동구유예방ANP적효응.
Objective To investigate the effect of pretreatment of pioglitazone on acute necrotizing pancreatitis (ANP) rats. Methods ANP was induced by retrograde injection of 5% sedinm deoxycholate into bilio-pancreatic ducts. The animals were randomly divided into ANP (n=18), sham operation (n=18) and pioglitazone pretreatment group (n=18). Pioglitazone was given 20 mg/kg in pioglitazone group before ANP was induced. The rats were sacrificed 3 h, 6 h, 12 h after ANP induction, respectively. Bblood samples were taken for serum amylase measurement. Tissue samples of pancreas were harvested for morphological observation under conventional light microscopy. Pathological change of pancreas was evaluated by Hughes and Kusske score system. Results The concentration of serum amylase and the pancreatic histological score in pioglitazone and ANP groups were significantly higher than those in sham operation group (P＜0.001 ). The concentration of serum amylase, Hughes and Kusske score in pioglitazone group at 12 h after ANP induction were (2980± 1080) U/L,4.50±2.07 and 7.50±1.05, respectively, and were lower than (7598±1072) U/L, 7.17±1.47 and 11.33±1.75 of ANP group at 12 h (P＜0.01). Conclusions Pioglitazone pretreatment could decrease the serum level of amylase and the pancreatic histological score. Pioglitazone may ameliorate the severity of ANP.
