CAJ | 학술논문

目的探讨瑞舒伐他汀对氧化修饰低密度脂蛋白(ox-LDL)诱导的人单核-巨噬细胞组织因子(TF)表达影响及可能机制.方法据实验要求分空白对照组、ox-LDL组、多聚肌苷酸组、瑞舒伐他汀组.RT-PCR检测各组血凝素样氧化低密度脂蛋白受体-1(LOX-1) mRNA,TFmRNA表达,ELISA检测各组TF蛋白表达.结果 ox-LDL组与空白对照组比较,LOX-1 mRNA、TFmRNA表达增加[ (3.25156±0.15772)vs(1±0)；(2.522451±0.138967) vs(1±0)],其差异均有统计学意义(P＜0.01).多聚肌苷酸组、瑞舒伐他汀组与ox-LDL组比较,LOX-1 mRNA、TFmRNA表达减少[ (2.95139±0.157253) vs(3.25156±0.15772)、(2.877343±0.156558) vs(3.25156±0.15772);(1.811956±0.169699)vs(2.522451±0.138967)、(1.687701±0.174647) vs(2.522451±0.138967)],其差异均有统计学意义(P＜0.05).ox-LDL组与空白对照组比较,TF蛋白表达增加[ (207.7233±1.154701)vs(184.8467±0.871799)],差异有统计学意义(P＜0.01).多聚肌苷酸组、瑞舒伐他汀组与ox-LDL组比较,TF蛋白表达均减少[(197.8733±1.505003) vs(207.7233±1.154701)、(202.9567±2.722744)vs(207.7233±1.154701)],差异均有统计学意义(P＜0.05).结论 LOX-1可能介导ox-LDL诱导的人单核-巨噬细胞TF表达增加.瑞舒伐他汀可通过下调单核-巨噬细胞LOX-1 mRNA的表达下调TFmRNA表达,从而下调TF蛋白表达.
목적 탐토서서벌타정대양화수식저밀도지단백(ox-LDL)유도적인단핵-거서세포조직인자(TF)표체영향급가능궤제.방법 거실험요구분공백대조조、ox-LDL조、다취기감산조、서서벌타정조.RT-PCR검측각조혈응소양양화저밀도지단백수체-1(LOX-1) mRNA,TFmRNA표체,ELISA검측각조TF단백표체.결과 ox-LDL조여공백대조조비교,LOX-1 mRNA、TFmRNA표체증가[ (3.25156±0.15772)vs(1±0)；(2.522451±0.138967) vs(1±0)],기차이균유통계학의의(P＜0.01).다취기감산조、서서벌타정조여ox-LDL조비교,LOX-1 mRNA、TFmRNA표체감소[ (2.95139±0.157253) vs(3.25156±0.15772)、(2.877343±0.156558) vs(3.25156±0.15772);(1.811956±0.169699)vs(2.522451±0.138967)、(1.687701±0.174647) vs(2.522451±0.138967)],기차이균유통계학의의(P＜0.05).ox-LDL조여공백대조조비교,TF단백표체증가[ (207.7233±1.154701)vs(184.8467±0.871799)],차이유통계학의의(P＜0.01).다취기감산조、서서벌타정조여ox-LDL조비교,TF단백표체균감소[(197.8733±1.505003) vs(207.7233±1.154701)、(202.9567±2.722744)vs(207.7233±1.154701)],차이균유통계학의의(P＜0.05).결론 LOX-1가능개도ox-LDL유도적인단핵-거서세포TF표체증가.서서벌타정가통과하조단핵-거서세포LOX-1 mRNA적표체하조TFmRNA표체,종이하조TF단백표체.
Objective To investigate the effects and mechanism of rosuvastatin on the expression of tissue factor in cultured human monocyte-macrophages cells which was induced by oxidized low density lipoprotein(ox-LDL).Methods The human monocyte-macrophages cells were divided into four groups:control group,ox-LDL group,Poly-inosine monophosphate (Poly Ⅰ) group,rosuvastatin group.The expression of LOX-1mRNA and TF mRNA was assayed by RT-PCR.The ELISA was performed to determine the protein concentration of TF.Results Compared with control group,the expression of LOX-1 mRNA and TF mRNA was increased in the ox-LDL group[ (3.25156±0.15772) vs (1±0) ; (2.522451±0.138967) vs (1±0) ],and it was in a concentration-dependent manner (P＜0.01).Compared with the expression of LOX-1 mRNA in the Poly-inosine monophosphate group and rosuvastatin group,TF mRNA were decreased in the ox-LDL group[ (2.95139±0.157253) vs(3.25156±0.15772) ; (2.877343±0.156558) vs(3.25156±0.15772) ; (1.811956±0.169699) vs (2.522451±0.138967) ; (1.687701±0.174647) vs (2.522451±0.138967)],and it was in a concentration-dependent manner(P＜0.05).Compared with control group,the expression of TF in the ox-LDL group was increased [(207.7233±1.154701) vs (184.8467±0.871799) ],and it was in a concentration-dependent manner (P＜0.01).Compared with the Poly-inosine monophosphate group and rosuvastatin group [(197.8733±1.505003) vs (207.7233±1.154701) ;(202.9567±2.722744)vs(207.7233±1.154701) ],the expression of TF in the ox-LDL group were decreased,and it was in a concentration-dependent manner (P＜0.05).Conclusions LOX-1 may be responsible for the expression of TF in human monocyte-macrophages cells induced by ox-LDL.Rosuvastatin is able to down-regulate the expression of LOX-1mRNA in human monocyte-macrophages cells through oxLDL,and TF mRNA and TF expression can be reduced.