CAJ | 학술논문

目的经体外细胞毒性测试初步筛选一类新型的聚醚型聚氨酯作为可注射型人工髓核材料.方法设置5个实验组:即样品1、2、3组为加入不同配比催化剂合成的软硬段配比不同的聚醚型聚氨酯;样品4组是不加催化剂,在37℃下经缓慢聚合而成的聚醚型聚氨酯;样品5组为单体有毒基团完全反应的聚醚型聚氨酯.另设1个对照组为不含材料浸提液的培养基.称取每组实验材料各0.6mg,经消毒后分别制成浸提液,经稀释50%后培养小鼠成纤维细胞(L929细胞),分别培养至第3天、4天、5天,使用3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四唑(MTT)比色法比较材料组与对照组间酶联免疫分析法测得的490 nm处的OD值,计算细胞相对增殖率,来评价材料的细胞毒性.结果样品4组和样品5组与对照组相比,L929细胞相对增殖率没有显著性差异,细胞毒性分级属于0～1级;样品1～3组与对照组L929细胞相对增殖率比较,差异有统计学意义,细胞毒性1～2级,但细胞形态正常.结论未加催化剂的体内聚合聚氨酯材料没有细胞毒性,具有作为髓核替代材料的应用潜力.加入催化剂后快速聚合的材料毒性有待进一步测试.
목적 경체외세포독성측시초보사선일류신형적취미형취안지작위가주사형인공수핵재료.방법 설치5개실험조:즉양품1、2、3조위가입불동배비최화제합성적연경단배비불동적취미형취안지;양품4조시불가최화제,재37℃하경완만취합이성적취미형취안지;양품5조위단체유독기단완전반응적취미형취안지.령설1개대조조위불함재료침제액적배양기.칭취매조실험재료각0.6mg,경소독후분별제성침제액,경희석50%후배양소서성섬유세포(L929세포),분별배양지제3천、4천、5천,사용3-(4,5-이갑기-2-새서)-2,5-이분기추화사서(MTT)비색법비교재료조여대조조간매련면역분석법측득적490 nm처적OD치,계산세포상대증식솔,래평개재료적세포독성.결과 양품4조화양품5조여대조조상비,L929세포상대증식솔몰유현저성차이,세포독성분급속우0～1급;양품1～3조여대조조L929세포상대증식솔비교,차이유통계학의의,세포독성1～2급,단세포형태정상.결론 미가최화제적체내취합취안지재료몰유세포독성,구유작위수핵체대재료적응용잠력.가입최화제후쾌속취합적재료독성유대진일보측시.
To sieve a new type of polyether polyurethane as an injectable prosthetic nucleus pulpous through testing the cytotoxicity in vitro.Methods Five experimental groups were set as following:that samples 1,2,3 groups were polyether-polyurethane polymerized of different ratio of soft and hard segment with different catalysts; group 4 was polyether polyurethane polymerized slowly at 37 ℃ without catalyst; group 5was polyether polyurethane that toxic monomer responded completely.Additionally,a control group of culture medium was set without material extracts.Get 0.6 mg from each sample product and make into material extracts after disinfection,then dilution them by 50% and culture the mouse fibroblasts(cell L929)until 3 d,4d,5 d,by Adopting 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide(MTT)colorimetric method to compare the 490 nm OD between the materials and control groups obtained by enzyme linked immune analysis,calculate the RGR of cells,and then evaluating the cytotoxicity of the materials.Results The RGR of L929 cell in group 4 and group 5 have no significant difference compared to the control group.Cytotoxic levels were 0 to 1; difference of the RGR of L929 cell between group 1-3 and control group were statistically significant.Cytotoxic levels were 1 to 2,but the cell morphology was normal.Conclusion The material polymerized without catalyst does not show cytotoxicity,and has great potential to be used as a substitute for nucleus pulposus.The cytotoxicity of the materials polymerized with catalyst needs to be tested more.