中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2011年
35期
10-12
,共3页
乳腺肿瘤%肥胖症%过氧化物酶增殖体受体γ
乳腺腫瘤%肥胖癥%過氧化物酶增殖體受體γ
유선종류%비반증%과양화물매증식체수체γ
Breast neoplasms%Obesity%Peroxisome proliferator-activated receptor gamma
目的 探讨过氧化物酶增殖体受体γ(PPARγ)在肥胖乳腺癌患者乳腺癌组织中的表达及临床意义.方法 采集60例肥胖乳腺癌患者(肥胖组),60例正常体重乳腺癌患者(正常体重组),用反转录-聚合酶链反应(RT-PCR)和免疫组织化学法检测两组乳腺癌组织PPARγ的mRNA和蛋白表达.结果 肥胖组乳腺癌组织PPARγ的mRNA和蛋白表达分别为0.79±0.06、0.55±0.07,均高于正常体重组的0.42±0.04、0.23±0.03,差异有统计学意义(P＜ 0.05),并且PPARγ mRNA和蛋白表达呈正相关(r=0.81,P＜0.05).肥胖组PPARγ阳性47例患者中,10例(21.3％)发生复发转移；而PPARγ阴性13例患者中,11例(84.6％)发生复发转移,差异有统计学意义(P＜0.05)；正常体重组PPARγ阳性19例患者中,4例(21.1％)发生复发转移；而PPARγ阴性41例患者中,10例(24.4％)发生复发转移,差异无统计学意义(P＞0.05).结论 PPARγ在肥胖乳腺癌患者中具有高表达趋势,提示PPARγ可能在绝经后肥胖相关性乳腺癌的发生、演变及预后中起重要作用.
目的 探討過氧化物酶增殖體受體γ(PPARγ)在肥胖乳腺癌患者乳腺癌組織中的錶達及臨床意義.方法 採集60例肥胖乳腺癌患者(肥胖組),60例正常體重乳腺癌患者(正常體重組),用反轉錄-聚閤酶鏈反應(RT-PCR)和免疫組織化學法檢測兩組乳腺癌組織PPARγ的mRNA和蛋白錶達.結果 肥胖組乳腺癌組織PPARγ的mRNA和蛋白錶達分彆為0.79±0.06、0.55±0.07,均高于正常體重組的0.42±0.04、0.23±0.03,差異有統計學意義(P＜ 0.05),併且PPARγ mRNA和蛋白錶達呈正相關(r=0.81,P＜0.05).肥胖組PPARγ暘性47例患者中,10例(21.3％)髮生複髮轉移；而PPARγ陰性13例患者中,11例(84.6％)髮生複髮轉移,差異有統計學意義(P＜0.05)；正常體重組PPARγ暘性19例患者中,4例(21.1％)髮生複髮轉移；而PPARγ陰性41例患者中,10例(24.4％)髮生複髮轉移,差異無統計學意義(P＞0.05).結論 PPARγ在肥胖乳腺癌患者中具有高錶達趨勢,提示PPARγ可能在絕經後肥胖相關性乳腺癌的髮生、縯變及預後中起重要作用.
목적 탐토과양화물매증식체수체γ(PPARγ)재비반유선암환자유선암조직중적표체급림상의의.방법 채집60례비반유선암환자(비반조),60례정상체중유선암환자(정상체중조),용반전록-취합매련반응(RT-PCR)화면역조직화학법검측량조유선암조직PPARγ적mRNA화단백표체.결과 비반조유선암조직PPARγ적mRNA화단백표체분별위0.79±0.06、0.55±0.07,균고우정상체중조적0.42±0.04、0.23±0.03,차이유통계학의의(P＜ 0.05),병차PPARγ mRNA화단백표체정정상관(r=0.81,P＜0.05).비반조PPARγ양성47례환자중,10례(21.3％)발생복발전이；이PPARγ음성13례환자중,11례(84.6％)발생복발전이,차이유통계학의의(P＜0.05)；정상체중조PPARγ양성19례환자중,4례(21.1％)발생복발전이；이PPARγ음성41례환자중,10례(24.4％)발생복발전이,차이무통계학의의(P＞0.05).결론 PPARγ재비반유선암환자중구유고표체추세,제시PPARγ가능재절경후비반상관성유선암적발생、연변급예후중기중요작용.
Objective To explore the expression and clinical significance of peroxisome proliferator-activated receptor gamma (PPAR γ) in breast tissue in obese patients with breast cancer.Methods Breast tissue samples were collected from 60 obese patients with breast cancer (obese group),60 normal weight patients with breast cancer (normal weight group),the expression of PPAR γmRNA and protein of breast tissue were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry in all patients,and the data was analyzed between PPARγ mRNA and protein.Results Compared with normal weight group,the expression of PPAR γ mRNA and protein of breast tissue in obese group were increased obviously(0.79 ± 0.06 vs 0.42 ± 0.04,0.55 ± 0.07 vs 0.23 ± 0.03 )(P＜ 0.05 ),while the expression of PPAR 3 mRNA and protein were positively correlated (r =0.81,P＜ 0.05 ).In obese group,the positive expression of PPAR γ was 47 cases with 10 cases (21.3％) recurrence and metastasis,the negative expression of PPAR 3 was 13 cases with 11 cases (84.6％) recurrence and metastaais,there was significant difference(P ＜ 0.05 ).In normal weight group,the positive expression of PPAR γwas 19 cases with 4 cases ( 21.1％ ) recurrence and metastasis,the negative expression of PPAR 3 was 41 cases with 10 cases (24.4％) recurrence and metastasis,there was no significant difference (P ＞ 0.05 ).Conclusions Obese patients with breast cancer own a high expression trend of expression of PPAR γ mRNA and protein of breast tissue,which indicates that PPARγ may be play an important role in occurrence,evolvement and prognosis of obesity-related breast cancer.
