CAJ | 학술논문

目的研究促性腺激素释放激素激动剂(GnRHa)抑那通对注射卡铂大鼠生殖功能的保护作用.方法 40只雌性Wistar大鼠分为4组,即卡铂组,卡铂+抑那通组,抑那通组,正常对照组;每组10只,分别接受卡铂、卡铂+抑那通、抑那通和生理盐水腹腔注射.实验结束后经腹主动脉采血,测定大鼠血清中雌二醇(E2)、卵泡刺激素(FSH)水平;摘除双侧卵巢和子宫,分别称湿质量并在光镜下进行形态学和病理学观察,进行各级卵泡记数,典型图像照相等.结果卡铂组与正常对照组比较,体质量均明显减轻(均P<0.05),卵巢及子宫湿质量均明显下降(均P<0.05),各级卵泡数目明显减少(均P<0.05),且伴有间质纤维化,坏死,细胞呈退行性变化,体积缩小,空泡样改变,黄体纤维化,血管减少;FSH明显升高(P<0.05),而E2值显著下降(P<0.05);卡铂+抑那通组初级卵泡数量明显多于卡铂组(P<0.05),且未见间质细胞纤维化或坏死.结论抑那通的先期应用对预防化疗所致的大鼠生殖功能损伤有一定的保护作用.
목적 연구촉성선격소석방격소격동제(GnRHa)억나통대주사잡박대서생식공능적보호작용.방법 40지자성Wistar대서분위4조,즉잡박조,잡박+억나통조,억나통조,정상대조조;매조10지,분별접수잡박、잡박+억나통、억나통화생리염수복강주사.실험결속후경복주동맥채혈,측정대서혈청중자이순(E2)、란포자격소(FSH)수평;적제쌍측란소화자궁,분별칭습질량병재광경하진행형태학화병이학관찰,진행각급란포기수,전형도상조상등.결과 잡박조여정상대조조비교,체질량균명현감경(균P<0.05),란소급자궁습질량균명현하강(균P<0.05),각급란포수목명현감소(균P<0.05),차반유간질섬유화,배사,세포정퇴행성변화,체적축소,공포양개변,황체섬유화,혈관감소;FSH명현승고(P<0.05),이E2치현저하강(P<0.05);잡박+억나통조초급란포수량명현다우잡박조(P<0.05),차미견간질세포섬유화혹배사.결론 억나통적선기응용대예방화료소치적대서생식공능손상유일정적보호작용.
Objective To study the effect of gonadotropin releasing hormone agonist(GnRHa)against car-boplation-induced gonadotoxicity in rats. Methods Forty Wistar rats were divided into four groups which received carboplation, GnRHa + carboplation, GnRHa and normal saline respectively(n=10 for each group). Blood samples were collected from the abdominal aorta and the levels of blood follicle stimulation hormone (FSH) and estradiol (E<2>) were determined. Both ovaries and uterus of each rat were removed to measure the amount and the maturity of follicles. Body mass and morphological and pathological features of the rats were also observed. Results Compared with that in control group, the body mass of ovary and uterus decreased (P<0.05), and a significant reduction was observed in the number of ovarian follicles at each grade (P<0.05). The levels of E2 significantly lowered (P<0.05) and the level of FSH markedly ascended in group carboplation. Compared with that in group carboplation, the amount of primitive follicles significantly increased in group GnRHa + carboplation (P<0.05), and carboplation showed markedly protective effect on the ovarian and uterine morphological construction of rats. Conclusion Gn-Rha, appliying to preventing the rat reproduction damage in advance, has the certain protective function.