CAJ | 학술논문

目的通过对乙肝病毒(HBV)母婴垂直传播者胎盘超微结构的观察，探讨HBV母婴垂直传播的细胞学机制。方法用ELISA双抗夹心法对孕妇静脉血及其胎儿脐血、新生儿静脉血进行HBV检测。对5例发生HBV母婴垂直传播者胎盘用JEM-1200EM透射电镜观察超微结构变化。结果HBV双抗原阳性孕妇60%发生HBV母婴垂直传播。HBV母婴垂直传播胎盘的超微结构改变有：胎盘合体细胞内核糖体增多，微绒毛增多、变形，合体细胞变性坏死；胎盘屏障中毛细血管基底膜增厚、纤维蛋白样物沉积，合体细胞间隙、胞浆、细胞核内见多种病毒样颗粒。结论HBV可感染胎盘合体细胞并且在其中复制，从而使胎儿在宫内被感染。
목적통과대을간병독(HBV)모영수직전파자태반초미결구적관찰，탐토HBV모영수직전파적세포학궤제。방법용ELISA쌍항협심법대잉부정맥혈급기태인제혈、신생인정맥혈진행HBV검측。대5례발생HBV모영수직전파자태반용JEM-1200EM투사전경관찰초미결구변화。결과HBV쌍항원양성잉부60%발생HBV모영수직전파。HBV모영수직전파태반적초미결구개변유：태반합체세포내핵당체증다，미융모증다、변형，합체세포변성배사；태반병장중모세혈관기저막증후、섬유단백양물침적，합체세포간극、포장、세포핵내견다충병독양과립。결론HBV가감염태반합체세포병차재기중복제，종이사태인재궁내피감염。
Objective By observing the placental ultramicrostructure in thefetuses infected by HBV in uterus from their mothers, to investigate the cellular mechanism of HBV transmitting. Methods HBV was determined in mother' serum,cord serum and neonatal serum with ELISA. Five patients'placentae ultramicrostructure of which HBV was transmitted to the fetuses were observed with JEM-1200EM. Results The HBV transmitting rate from mother to fetus in HBV antigens both positive women was 60%. The changes of HBV transmitting placentae include the increasing of ribosome and shape changes of microvilli, the trophoblastic necrosis, the capillary basal lamina in placenta barrier thickness with fibrin-like substance deposits and the exsistance of HBV-like particles in cell spaces, plasma and nuclears. Conclusions HBV can be transmitted from mother to fetus in uterus by infecting and duplicating in the syncytial trophoblast.