CAJ | 학술논문

目的对浙江省临床分离猪链球菌血清2型(SS2)菌株进行89K候选致病岛检测.方法用多种特异性引物进行PCR扩增检测,同时对89K的3个扩增片段进行测序,并结合有关资料进行生物信息学和流行病学分析.结果 8株SS2菌株,均检出89K(1&2)、89K(3&4)、89K(5&6)候选致病岛特异片段和种特异性16S rDNA、cps2J、mrp毒力基因,和1998年江苏省暴发疫情的菌株结果一致.ZJ0501号SS2分离株89K候选致病岛的3个PCR扩增片段序列和1998年江苏省暴发疫情的菌株有99%以上的相似性,其中编码DNA重组蛋白的序列片段和病乳链球菌及无乳链球菌有较高的同源性.结论近年来在浙江省临床分离Ss2菌株中,存在89K候选致病岛片段.
목적 대절강성림상분리저련구균혈청2형(SS2)균주진행89K후선치병도검측.방법 용다충특이성인물진행PCR확증검측,동시대89K적3개확증편단진행측서,병결합유관자료진행생물신식학화류행병학분석.결과 8주SS2균주,균검출89K(1&2)、89K(3&4)、89K(5&6)후선치병도특이편단화충특이성16S rDNA、cps2J、mrp독력기인,화1998년강소성폭발역정적균주결과일치.ZJ0501호SS2분리주89K후선치병도적3개PCR확증편단서렬화1998년강소성폭발역정적균주유99%이상적상사성,기중편마DNA중조단백적서렬편단화병유련구균급무유련구균유교고적동원성.결론 근년래재절강성림상분리Ss2균주중,존재89K후선치병도편단.
Objective To identify the presence of candidate pathogenicity island 89K DNA sequence of Streptococcus suis serotype 2 (SS2) strains isolated from patient in Zhejiang province. Methods Genes and DNA fragments were amplified by PCR, using specific primers, and three amplified fragments of the89K sequence were directly sequenced. The results were analyzed using software related to bioinformaties and epidemiology. Results 8 strains of SS2 all contained 89K sequence, cps2J and mrp virulent genes, and species-specific 16S rDNA. 3 amplified fragments of 89K candidate pathogenicity island of SS2 ZJ0501 were above 99% similar to SS2 strain identified from outbreaks in Jiangsu in 1998, and the gene fragment of coding DNA recombinant protein in the 89K sequence was highly homological with that of S. dysgalactiae and S. agalactiae. Conclusion In recent years SS2 strains isolaed from patients with clinical symptoms in Zhejiang province had been detected to have contained candidate pathogenicy 89K DNA fragment.