CAJ | 학술논문

目的探讨汉族人群中外周血儿茶酚氧位甲基转移酶(COMT)基因表达与强迫症的关系.方法采用实时定量逆转录-聚合酶链反应技术检测35例强迫症首次发病患者(强迫症组)与31名健康对照(对照组)外周血COMT基因表达水平;使用美国精神障碍诊断与统计手册第4版轴Ⅱ诊断结构式临床访谈问卷(SCID-Ⅱ)评估强迫症与强迫性人格障碍的共病情况.结果强迫症组COMT基因表达水平明显低于对照组(t=2.56,P＜0.05),下调约32%;无强迫人格患者(15例)与对照组间COMT基因表达水平的差异有统计学意义(t=2.41,P＜0.05),而伴强迫人格患者(20例)与对照组的差异无统计学意义(t=1.50,P＞0.05);伴强迫人格与无强迫人格的强迫症患者COMT基因表达水平分别下调14%和56%;COMT表达水平与症状严重度无显著相关(r＝0.09,P＜0.05).结论 COMT基因表达下调可能与强迫症发病有关;伴或无强迫人格患者的遗传机制可能存在差异.
목적 탐토한족인군중외주혈인다분양위갑기전이매(COMT)기인표체여강박증적관계.방법 채용실시정량역전록-취합매련반응기술검측35례강박증수차발병환자(강박증조)여31명건강대조(대조조)외주혈COMT기인표체수평;사용미국정신장애진단여통계수책제4판축Ⅱ진단결구식림상방담문권(SCID-Ⅱ)평고강박증여강박성인격장애적공병정황.결과 강박증조COMT기인표체수평명현저우대조조(t=2.56,P＜0.05),하조약32%;무강박인격환자(15례)여대조조간COMT기인표체수평적차이유통계학의의(t=2.41,P＜0.05),이반강박인격환자(20례)여대조조적차이무통계학의의(t=1.50,P＞0.05);반강박인격여무강박인격적강박증환자COMT기인표체수평분별하조14%화56%;COMT표체수평여증상엄중도무현저상관(r＝0.09,P＜0.05).결론 COMT기인표체하조가능여강박증발병유관;반혹무강박인격환자적유전궤제가능존재차이.
Objective To explore the association of peripheral catechol-O-methyl-transferase (COMT)gene expression and obsessive-compulsive disorder(OCD)in Han Chinese.Methods Peripheral blood samples were collected from individuals with OCD(n=35)and age-and sex-matched healthy subjects (n=31).Relative COMT gene expression levels were examined with real-time quantitative reverse transcription PCR in peripheral blood of all the subjects.Structured clinical interview for the 4th Edition of Diagnostic and Statistical Manual of Mental Disorder axisⅡ(SCID-Ⅱ)was used to assess the co-morbidity of OCD and obsessive-compulsive personality disorders(OCPD).The Yale-Brown Obsessive-Compulsive Scale(Y-BOCS)was used to evaluate the severity of obsessive compulsive symptoms.Results The COMT gene expression levels were significantly decreased in the OCD group compared with healthy control group (t=2.56,P＜0.05).The OCD patients showed a 32%down-regulation of COMT gene expression.A significant lower COMT gene expression was observed in non-OCPD patients(n=15)in comparison with healthy controls(t=2.41.P＜0.05),while there was,no significant difference between OCPD patients (n=20)and healthy controls(t=1.50,P＞0.05).The rates of COMT gene expression down-regulation in OCD patients with OCPD and ones without OCPD were 14%and 56%respectively.There was no significant correlation between COMT expression level and the Y-BOCS score(r=0.09,P=0.62).Conclusions These results suggest that COMT gene expression down-regulation is associated with OCD,and the OCD patients with and without OCPD may have different genetic background.