中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2008年
3期
196-201
,共6页
张鹏飞%李继福%张梅%刘春喜%丁士芳%梁向明%徐新生%张彦恩%崔先泉%李晓楠%张运
張鵬飛%李繼福%張梅%劉春喜%丁士芳%樑嚮明%徐新生%張彥恩%崔先泉%李曉楠%張運
장붕비%리계복%장매%류춘희%정사방%량향명%서신생%장언은%최선천%리효남%장운
肾动脉%动脉粥样硬化%动物,实验%介入性技术%血管内超声%小型猪
腎動脈%動脈粥樣硬化%動物,實驗%介入性技術%血管內超聲%小型豬
신동맥%동맥죽양경화%동물,실험%개입성기술%혈관내초성%소형저
Renal artery%Atherosclerosis%Animal,experimental%Intervention%Intravaseular ultrasonography%Mini pig
目的 建立一种适于介入性技术研究的肾动脉粥样硬化小型猪动物模型.方法 纯种系中国实验用小型猪(CEMP)8只,饲以高脂饮食13周后,以过大球囊拉伤单侧肾动脉,并继续高脂饲料喂养至40周.分别测定第1、14、40周的血脂和血肌酐水平,并于第14和40周行血管内超声检查.40周处死动物后,采集标本行苏木素-伊红、Masson三色和油红O染色及抗巨噬细胞免疫组织化学染色.结果 第40周CEMP血脂和血肌酐水平较第1周明显升高,损伤侧肾脏出现灶性缺血性病理改变.CEMP肾动脉适于血管造影和介入性血管内超声检查,后者提供了清晰的血管截面病变信息,损伤侧肾动脉内膜-中层厚度与未损伤侧比较,差异有统计学意义[(0.89±0.03)mm比(0.30±0.02)mm,P＜0.05].病理检查证实肾动脉病变符合粥样硬化特征,形成的斑块中,纤维性占85%,纤维脂质性占15%.结论 成功建立了模拟动脉粥样硬化性肾血管病变发生发展和适于介入性技术研究的肾动脉粥样硬化小型猪动物模型.血管内超声技术对于动脉粥样硬化性肾血管病变的评价具有潜在的临床应用前景.
目的 建立一種適于介入性技術研究的腎動脈粥樣硬化小型豬動物模型.方法 純種繫中國實驗用小型豬(CEMP)8隻,飼以高脂飲食13週後,以過大毬囊拉傷單側腎動脈,併繼續高脂飼料餵養至40週.分彆測定第1、14、40週的血脂和血肌酐水平,併于第14和40週行血管內超聲檢查.40週處死動物後,採集標本行囌木素-伊紅、Masson三色和油紅O染色及抗巨噬細胞免疫組織化學染色.結果 第40週CEMP血脂和血肌酐水平較第1週明顯升高,損傷側腎髒齣現竈性缺血性病理改變.CEMP腎動脈適于血管造影和介入性血管內超聲檢查,後者提供瞭清晰的血管截麵病變信息,損傷側腎動脈內膜-中層厚度與未損傷側比較,差異有統計學意義[(0.89±0.03)mm比(0.30±0.02)mm,P＜0.05].病理檢查證實腎動脈病變符閤粥樣硬化特徵,形成的斑塊中,纖維性佔85%,纖維脂質性佔15%.結論 成功建立瞭模擬動脈粥樣硬化性腎血管病變髮生髮展和適于介入性技術研究的腎動脈粥樣硬化小型豬動物模型.血管內超聲技術對于動脈粥樣硬化性腎血管病變的評價具有潛在的臨床應用前景.
목적 건립일충괄우개입성기술연구적신동맥죽양경화소형저동물모형.방법 순충계중국실험용소형저(CEMP)8지,사이고지음식13주후,이과대구낭랍상단측신동맥,병계속고지사료위양지40주.분별측정제1、14、40주적혈지화혈기항수평,병우제14화40주행혈관내초성검사.40주처사동물후,채집표본행소목소-이홍、Masson삼색화유홍O염색급항거서세포면역조직화학염색.결과 제40주CEMP혈지화혈기항수평교제1주명현승고,손상측신장출현조성결혈성병리개변.CEMP신동맥괄우혈관조영화개입성혈관내초성검사,후자제공료청석적혈관절면병변신식,손상측신동맥내막-중층후도여미손상측비교,차이유통계학의의[(0.89±0.03)mm비(0.30±0.02)mm,P＜0.05].병리검사증실신동맥병변부합죽양경화특정,형성적반괴중,섬유성점85%,섬유지질성점15%.결론 성공건립료모의동맥죽양경화성신혈관병변발생발전화괄우개입성기술연구적신동맥죽양경화소형저동물모형.혈관내초성기술대우동맥죽양경화성신혈관병변적평개구유잠재적림상응용전경.
Objective To establish a mini pig model suitable for interventional studies in vivo. Methods The endothelia of unilateral renal arteries in 8 purebred Chinese experimental mini pigs(CEMP)was denuded by inflated balloons after the animals were fed with high cholesterol diet for 13 weeks.The CEMP were fed with h high cholesterol diet continuously till the 40th week.The levels of blood lipid panel and creatinine were tested at week 1,14 and 40.Bilateral renal arteries were examined with intravascular uhrasonography at week 14 and 40.The vessel samples were collected at week 40 and stained with haematoxylin-eosin,Masson trichrome technique, oil O and anti-macrophage immunohistological technique. Results Significant differences of blood lipid panel and creatinine were found between week 1 and week 40.Focal ischemic renal injury could be observed pathologically.Renal arteries of CEMP were suitable for interventional procedure such as angiography and intravascular ultrasonography.Cross-sectional information of vessels could be provided clearly by intravascular ultrasonography and the intimamedian thickness of injured renal arteries was much thicker than that of non-injured ones[(0.89±0.03)mm vs (0.30±0.02)mm,P＜0.05]as evidenced by this diagnostic technique.Pathological findings demonstrated the atheroselerotic profiles of the injured renal arteries.Fibrous and fibro-fatty plaques were the main pathologic types in this CEMP model. Conclusions An animal model with renal arterial atherosclerosis mimicking the progression of atheroselerotic renovaseular disease,which is suitable for interventional procedure is established successfully.Intravascular ultasonography may have potential clinical prospect on the evaluation of atherosclerotic renovaseular disease.
