中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2010年
2期
126-130
,共5页
罗英饰%钱菊英%张磊%马剑英%黄东%樊冰%葛均波
囉英飾%錢菊英%張磊%馬劍英%黃東%樊冰%葛均波
라영식%전국영%장뢰%마검영%황동%번빙%갈균파
干细胞%冠状动脉疾病%血管成形术,经腔,经皮冠状动脉
榦細胞%冠狀動脈疾病%血管成形術,經腔,經皮冠狀動脈
간세포%관상동맥질병%혈관성형술,경강,경피관상동맥
Stem cells%Coronary disease%Angioplasty,transluminal,percutaneous coronary
目的 观察并比较正常人与不同类型冠心病患者外周血中内皮祖细胞(EPCs)数量的差异,以及不同类型冠心病患者在经皮冠状动脉介入治疗(PCI)前后外周血中EPCs的数量变化.方法 将患者分成4组:稳定性心绞痛组、不稳定性心绞痛组、急性ST段抬高型心肌梗死组及正常对照组.利用流式细胞术双色分析法检测对照组以及3组不同类型冠心病患者在PCI术前、术后即刻、术后24 h EPCs占外周血有核细胞的百分比,其中EPCs以CD133~+/VEGFR-2~+双标记阳性确定.结果 PCI术前,外周血中EPCs数量在稳定性心绞痛组为(0.043±0.043)%、不稳定性心绞痛组为(0.014±0.018)%、急性ST段抬高型心肌梗死组为(0.040±0.036)%,均明显低于正常对照组[(0.111%±0.078)%](均P＜0.01),不稳定性心绞痛组外周血中EPCs的数量明显低于稳定性心绞痛组(P＜0.05).不稳定性心绞痛组外周血中EPCs的数量在PCI术后24 h较术前明显增加[(0.054±0.045)%比(0.014±0.018)%,P＜0.01].稳定性心绞痛组及急性ST段抬高型心肌梗死组PCI术前、术后EPCs的数量差异无统计学意义.结论 外周血中EPCs数量的变化可能与冠心病的病变程度有关.PCI对外周血EPCs数量的影响可能与冠心病类型有关.
目的 觀察併比較正常人與不同類型冠心病患者外週血中內皮祖細胞(EPCs)數量的差異,以及不同類型冠心病患者在經皮冠狀動脈介入治療(PCI)前後外週血中EPCs的數量變化.方法 將患者分成4組:穩定性心絞痛組、不穩定性心絞痛組、急性ST段抬高型心肌梗死組及正常對照組.利用流式細胞術雙色分析法檢測對照組以及3組不同類型冠心病患者在PCI術前、術後即刻、術後24 h EPCs佔外週血有覈細胞的百分比,其中EPCs以CD133~+/VEGFR-2~+雙標記暘性確定.結果 PCI術前,外週血中EPCs數量在穩定性心絞痛組為(0.043±0.043)%、不穩定性心絞痛組為(0.014±0.018)%、急性ST段抬高型心肌梗死組為(0.040±0.036)%,均明顯低于正常對照組[(0.111%±0.078)%](均P＜0.01),不穩定性心絞痛組外週血中EPCs的數量明顯低于穩定性心絞痛組(P＜0.05).不穩定性心絞痛組外週血中EPCs的數量在PCI術後24 h較術前明顯增加[(0.054±0.045)%比(0.014±0.018)%,P＜0.01].穩定性心絞痛組及急性ST段抬高型心肌梗死組PCI術前、術後EPCs的數量差異無統計學意義.結論 外週血中EPCs數量的變化可能與冠心病的病變程度有關.PCI對外週血EPCs數量的影響可能與冠心病類型有關.
목적 관찰병비교정상인여불동류형관심병환자외주혈중내피조세포(EPCs)수량적차이,이급불동류형관심병환자재경피관상동맥개입치료(PCI)전후외주혈중EPCs적수량변화.방법 장환자분성4조:은정성심교통조、불은정성심교통조、급성ST단태고형심기경사조급정상대조조.이용류식세포술쌍색분석법검측대조조이급3조불동류형관심병환자재PCI술전、술후즉각、술후24 h EPCs점외주혈유핵세포적백분비,기중EPCs이CD133~+/VEGFR-2~+쌍표기양성학정.결과 PCI술전,외주혈중EPCs수량재은정성심교통조위(0.043±0.043)%、불은정성심교통조위(0.014±0.018)%、급성ST단태고형심기경사조위(0.040±0.036)%,균명현저우정상대조조[(0.111%±0.078)%](균P＜0.01),불은정성심교통조외주혈중EPCs적수량명현저우은정성심교통조(P＜0.05).불은정성심교통조외주혈중EPCs적수량재PCI술후24 h교술전명현증가[(0.054±0.045)%비(0.014±0.018)%,P＜0.01].은정성심교통조급급성ST단태고형심기경사조PCI술전、술후EPCs적수량차이무통계학의의.결론 외주혈중EPCs수량적변화가능여관심병적병변정도유관.PCI대외주혈EPCs수량적영향가능여관심병류형유관.
Objective To compare circulating endothelial progenitor cells(EPCs)number between normal controls and patients with coronary heart diseases(CHD),and to explore the influence of percutaneous coronary intervention(PCI)on the number of EPCs in patients with CHD.Methods A total of 48 hospitalized patients with CHD were enrolled and divided into three groups,including stable angina pectoris(SAP)group,unstable angina pectoris(UA)group,acute ST-elevation myocardial infarction (STEMI)group.Patients with normal coronary angiography served as controls.The percentage of EPCs in peripheral blood nucleated cells was measured at admission and immediately after and 24 hours after PCI in CHD patients by double-color flow cytometry analysis.EPCs were identified with CD133~+/VEGFR-2~+.Results At admission,the percentage of EPCs in peripheral blood nucleated cells was significantly lower in SAP group(0.043%±0.043%),UA group(0.014%±0.018%)and STEMI group(0.040%±0.036%)than that in the control group(0.111%±0.078%,all P＜0.01).The number of EPCs in UA group was significantly lower than that in the SAP group(P＜0.05).In the UA group,the number of EPCs at 24 hours after PCI(0.054%±0.045%)was significantly higher than before operation(0.014%±0.018%,P＜0.01)and tended to be higher than the value immediately after PCI(0.028%±0.041%,P＞0.05).The before and after PCI EPCs numbers wore similar in SAP and STEMI groups(all P＞0.05).Conclusion The number of peripheral EPCs in patients with CHD is lower than that in normal subjects and negatively related with severity of coronary heart disease.The number of circulating EPCs increased post PCI in patients with unstable angina pectoris.
