CAJ | 학술논문

大豆籽粒贮藏蛋白7S和11S组分及其亚基含量、11S/7S比值与大豆蛋白的营养价值和加工特性密切相关.获得具不同7S和11S组分及其亚基含量、不同11S/7S比值的种质材料是对大豆蛋白的营养价值和功能特性进行遗传育种改良的重要材料基础.本研究利用SDS-PAGE技术,对706份中国大豆种质资源7S、11S组分及其亚基相对含量进行了研究.结果表明:706份我国大豆种质资源中7S、11S组分及其亚基相对含量具有丰富的遗传变异;7S和11S组分含量间存在极显著的负相关(r=-1.00,P＜0.01);603份地方品种和103份新育成品种或主栽品种的7S、11S组分相对含量的平均值和变异幅度分别为40.00%,20.58%～56.65%,60.00%,43.35%～79.42%和38.21%,30.33%～52.67%,61.79%,47.33%～69.67%;11S/7S比值的平均值和变异幅度分别为1.54,0.77～3.86和1.65,0.90～2.30;筛选获得了63份7S、11S组分或亚基含量变异种质.
대두자립저장단백7S화11S조분급기아기함량、11S/7S비치여대두단백적영양개치화가공특성밀절상관.획득구불동7S화11S조분급기아기함량、불동11S/7S비치적충질재료시대대두단백적영양개치화공능특성진행유전육충개량적중요재료기출.본연구이용SDS-PAGE기술,대706빈중국대두충질자원7S、11S조분급기아기상대함량진행료연구.결과표명:706빈아국대두충질자원중7S、11S조분급기아기상대함량구유봉부적유전변이;7S화11S조분함량간존재겁현저적부상관(r=-1.00,P＜0.01);603빈지방품충화103빈신육성품충혹주재품충적7S、11S조분상대함량적평균치화변이폭도분별위40.00%,20.58%～56.65%,60.00%,43.35%～79.42%화38.21%,30.33%～52.67%,61.79%,47.33%～69.67%;11S/7S비치적평균치화변이폭도분별위1.54,0.77～3.86화1.65,0.90～2.30;사선획득료63빈7S、11S조분혹아기함량변이충질.
The results showed that 7S globulin was divided as three subunits (α',α,β), and 11S globulin was divided into three bands which were represented as A3, A1aA1bA2A4 (Acid subunits) and B1aB1bB2B3B4 (Basic subunits), respectively. There were great genetic diversity of the relative contents of 11S, 7S globulins and their subunits in 706 Chinese soybean germplasm. Remarkably negative correlation between the relative contents of 7S and 11S globulins (r=-1.00, P＜0.01)existed. The average and variation range of the relative content of 7S, 11S fraction of 603 Chinese landraces and 103 cultivars were 40.00%,20.58%～56.65%, 60.00%, 43.35%～79.42% and 38.21%, 30.33%～52.67%, 61.79%, 47.33%～69.67%, respectively. The average and variation range of the 11S/7S ratios of 603 Chinese landraces and 103 cultivars were 1.54, 0.77～3.86and 1.65, 0.90～2.30, respectively. 63 accessions screened from 706 germplasm showed polymorphism of band type for the subunit relative contents.