CAJ | 학술논문

以陈放1年的油菜蜂花粉为原料,对破壁脱脂后的花粉中各组分蛋白含量进行了研究,其中谷蛋白的含量约占总蛋白含量的55.7%,清蛋白为39.O%,球蛋白和醇蛋白只有3.2%和2.1%.以谷蛋白为原料,在单因素试验的基础上,利用响应面分析法对油菜花粉谷蛋白最佳酶解条件进行了研究.结果表明,Alcalase碱性蛋白酶水解油菜花粉谷蛋白的最佳水解条件为底物浓度6%,pH值9.0,水解温度60℃,酶底比1460U/g,水解时间2h,谷蛋白酶法改性后抗羟基自由基的活性能达到70.0%.
이진방1년적유채봉화분위원료,대파벽탈지후적화분중각조분단백함량진행료연구,기중곡단백적함량약점총단백함량적55.7%,청단백위39.O%,구단백화순단백지유3.2%화2.1%.이곡단백위원료,재단인소시험적기출상,이용향응면분석법대유채화분곡단백최가매해조건진행료연구.결과표명,Alcalase감성단백매수해유채화분곡단백적최가수해조건위저물농도6%,pH치9.0,수해온도60℃,매저비1460U/g,수해시간2h,곡단백매법개성후항간기자유기적활성능체도70.0%.
Protein fractionations of cell-fragmentated and defatted rape pollen stored for more than 12 months were investigated. Rape pollen glutelin was hydrolyzed with alcalase and the crude peptides of rape pollen were obtained. The scavenging effects of rape pollen glutelin and its hydrolysate on hydroxyl free radicals (·OH) in the deoxyribose-iron system was studied. Results show that glutelins and albumins are found to be the predominant proteins in rape pollen, accounting for 55.7% and 39.0% of total proteins, while globulins and prolamins are 3.2% and 2.1%. respectively. The optimum conditions of alcalase enzymatic hydrolysis were determined by single-factor analysis and response surface methodology as follows: pH9, hydrolyzing temperature 50℃, enzyme concentration 1460 U per gram of substrate, concentration of substrate 6%, hydrolyzing time 2 h. In the antioxidation test, the inhibition rate·OH of glutelin was 25.1%. After being hydrolyzed, the inhibition rate of·OH of glutelin oligopeptide was remarkably increased to reach 70.0%.