国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2008年
8期
689-690
,共2页
聂俊玮%梁燕%杨寿%唐玉芬
聶俊瑋%樑燕%楊壽%唐玉芬
섭준위%량연%양수%당옥분
淋巴细胞培养%生长因素%方法%转化率%分裂相
淋巴細胞培養%生長因素%方法%轉化率%分裂相
림파세포배양%생장인소%방법%전화솔%분렬상
Lymphocute cultivation%Growth factor%Method%Transformation ratio%Mitotic phase
目的 探讨影响淋巴细胞培养生长因素及去除方法.方法 在正常淋巴细胞培养中去掉原培养液,加入灭菌生理水(内含有青霉素钠100 IU/mL)洗涤后再加入培养基继续培养,常规收获细胞及常规G显带油镜观察分析.结果 用上述方法洗涤后收获的细胞较正常培养收获的细胞转化率、有丝分裂指数、分裂相质量均提高非常显著(P<0.01).结论 该方法可去除影响淋巴细胞培养生长的因素,保证收获细胞数量和质量,从而提高分析诊断水平,且易于推广应用.
目的 探討影響淋巴細胞培養生長因素及去除方法.方法 在正常淋巴細胞培養中去掉原培養液,加入滅菌生理水(內含有青黴素鈉100 IU/mL)洗滌後再加入培養基繼續培養,常規收穫細胞及常規G顯帶油鏡觀察分析.結果 用上述方法洗滌後收穫的細胞較正常培養收穫的細胞轉化率、有絲分裂指數、分裂相質量均提高非常顯著(P<0.01).結論 該方法可去除影響淋巴細胞培養生長的因素,保證收穫細胞數量和質量,從而提高分析診斷水平,且易于推廣應用.
목적 탐토영향림파세포배양생장인소급거제방법.방법 재정상림파세포배양중거도원배양액,가입멸균생리수(내함유청매소납100 IU/mL)세조후재가입배양기계속배양,상규수획세포급상규G현대유경관찰분석.결과 용상술방법세조후수획적세포교정상배양수획적세포전화솔、유사분렬지수、분렬상질량균제고비상현저(P<0.01).결론 해방법가거제영향림파세포배양생장적인소,보증수획세포수량화질량,종이제고분석진단수평,차역우추엄응용.
Objective To probe into the influential factor for lymphocyte growth and its removing method.Methods The original culture liquid in normal lymphocyte medium was removed and washed with the disinfectant physiological saline(containing 100 IU/mL of benzylpenicillin sodium).After that,the new culture medium was added for continuous cultivation.The lymphocytes had been collected conventionally and G banding analysis under immersion objective was performed.Results After the aforesaid washing method was used,cell transformation efficiency,mitotic index and quality of mitotic phase of lymphocytes were obviously improved compared with those cultivated by conventional cultivation method(P<0.01).Conclusion It is proved that this new method can remove the influence on lymphocyte growth during cultivation and guarantee the harvesting cell number and quality,and then improve the levels of analysis and diagnosis.And also it is easy for popularization.
