CAJ | 학술논문

对我国东南沿海日本囊对虾的4个养殖群体广东群体(GD)、台湾群体(TW)、福建群体(FJ)和浙江群体(ZJ)的线粒体细胞色素6基因片段进行PCR扩增,对产物进行测序后分析.经比对获得552bp的核苷酸序列,发现了52个变异位点,得到了50种单倍型.广东、台湾、福建和浙江群体的核苷酸多样性依次分别为0.0082、0.0044、0.0137、0.0045,各群体均存在较好的单倍型多态性和核苷酸多态性.群体遗传分化分析表明各群体间保持着一定的遗传差异,其中福建群体与其它群体之间存在明显的遗传分化.采用MEGA软件计算了群体间的遗传距离,福建群体与广东群体间的遗传距离最远0.012,台湾群体与浙江群体间的遗传距离最小为0.005.以其它4种对虾为外群构建了4个群体的NJ系统进化树,结果显示台湾群体与浙江群体最先聚为一组群.
대아국동남연해일본낭대하적4개양식군체엄동군체(GD)、태만군체(TW)、복건군체(FJ)화절강군체(ZJ)적선립체세포색소6기인편단진행PCR확증,대산물진행측서후분석.경비대획득552bp적핵감산서렬,발현료52개변이위점,득도료50충단배형.엄동、태만、복건화절강군체적핵감산다양성의차분별위0.0082、0.0044、0.0137、0.0045,각군체균존재교호적단배형다태성화핵감산다태성.군체유전분화분석표명각군체간보지착일정적유전차이,기중복건군체여기타군체지간존재명현적유전분화.채용MEGA연건계산료군체간적유전거리,복건군체여엄동군체간적유전거리최원0.012,태만군체여절강군체간적유전거리최소위0.005.이기타4충대하위외군구건료4개군체적NJ계통진화수,결과현시태만군체여절강군체최선취위일조군.
To investigate the variation of mitochondrial cytb sequence and assess the genetic structure and polymor-phism of four cultured stocks of Marsupenaeus japonicus, M. japonicus were sampled in South China Guangdong (GD),Taiwan (TW), Fujian (FJ), and Zhejiang (ZJ) provinces. With PCR technique, we amplified the mt-DNA cytb gene frag-ment among the four stocks. 552bp nucleotide sequences of partial cytb were obtained, of which 52 variable sites and 50 haplotypes were detected. Nucleotide diversities in GD, TW, FJ,and ZJ stocks were 0.0082, 0.0044, 0.0137, and 0.0045 respectively. All the stocks showed high diversity in nucleotide and haplotype. Genetic difference in all the four stocks was found, of which the one between FJ stock and other three stocks was significant, shown by F_(st) analysis. The Ki-mura-2-paramter genetic distance calculated by MEGA software between FJ and GD stocks is the greatest at 0.012, while that between ZJ and TW ones is the smallest at 0.005. A NJ phylogenetic tree was constructed using four penaeidae shrimp as out group. These results shall be very important and useful for making scientific strategy for the family selective breed-ing with molecular marker assistance.