CAJ | 학술논문

采用以羽毛粉为唯一碳源和氮源的培养基,从自然界中分离到一株能够高效降解羽毛角蛋白的细菌,经形态学观察,生理生化实验和16SrRNA基因鉴定,初步确定该菌株为短小芽孢杆菌(Bacillus pumilus),且命名为短小芽孢杆菌WHK4.发酵48 h时,羽毛粉降解率达到85.76%.本研究为微生物降解羽毛角蛋白提供了优良的菌株,在蛋白饲料生产中具有潜在的广泛的应用前景.
채용이우모분위유일탄원화담원적배양기,종자연계중분리도일주능구고효강해우모각단백적세균,경형태학관찰,생리생화실험화16SrRNA기인감정,초보학정해균주위단소아포간균(Bacillus pumilus),차명명위단소아포간균WHK4.발효48 h시,우모분강해솔체도85.76%.본연구위미생물강해우모각단백제공료우량적균주,재단백사료생산중구유잠재적엄범적응용전경.
A strain of degrading feather keratin bacterium with high efficiency was isolated from environment by adopting the culture medium taking feather meal as sole carbon and nitrogen source. This strain was identified as Bacillus pumilus by morphology, physiological and biochemical experiments and 16SrDNA gene analysis, and named as the Bacillus pumilus WHK4. The degradation rate of feather keratin reached to 85.76% after 48h fermentation. The results indicated that WHK4 was an appropriate strain for exploring microorganic fermentation system to degrade feather keratin, and had a great potential to be applied in producing high quality protein feed.