CAJ | 학술논문

目的了解制动对幼龄大鼠骨维生素D受体(vitamin D receptor,VDR)表达的影响,探讨废用性骨质疏松(disuse osteoporosis,DOP)与VDR表达的关系.方法通过腿-尾固定法对4周龄幼鼠分别进行右后肢2周和4周制动,采用股骨湿重、灰重、骨密度测量,骨组织病理学和骨组织形态学参数测定等方法对比评价制动对幼鼠制动侧股骨和胫骨的骨量、骨结构的影响,采用免疫组织化学和图象分析方法检测制动对幼鼠骨VDR表达的影响.结果幼鼠在制动2周和4周后制动侧股骨湿重、灰重、股骨和胫骨骨密度均低于同龄对照组(P＜0.05),骨组织切片可见制动侧胫骨近侧干骺端骨小梁稀疏、纤细和断裂,和相应同龄对照相比骨小梁面积和骨小梁周长降低(P＜0.05);免疫组织化学和图象分析方法显示幼鼠右后肢胫骨近侧干骺端骨组织成骨细胞胞浆和核膜有VDR表达,制动后表达增强.结论腿-尾固定法可成功建立幼鼠DOP动物模型;制动可增强幼鼠制动侧肢体骨VDR的表达,提示维生素D内分泌系统紊乱可能与DOP的发病机制有一定的关系.
목적 료해제동대유령대서골유생소D수체(vitamin D receptor,VDR)표체적영향,탐토폐용성골질소송(disuse osteoporosis,DOP)여VDR표체적관계.방법 통과퇴-미고정법대4주령유서분별진행우후지2주화4주제동,채용고골습중、회중、골밀도측량,골조직병이학화골조직형태학삼수측정등방법 대비평개제동대유서제동측고골화경골적골량、골결구적영향,채용면역조직화학화도상분석방법 검측제동대유서골VDR표체적영향.결과 유서재제동2주화4주후제동측고골습중、회중、고골화경골골밀도균저우동령대조조(P＜0.05),골조직절편가견제동측경골근측간후단골소량희소、섬세화단렬,화상응동령대조상비골소량면적화골소량주장강저(P＜0.05);면역조직화학화도상분석방법 현시유서우후지경골근측간후단골조직성골세포포장화핵막유VDR표체,제동후표체증강.결론 퇴-미고정법가성공건립유서DOP동물모형;제동가증강유서제동측지체골VDR적표체,제시유생소D내분비계통문란가능여DOP적발병궤제유일정적관계.
Objective To study the expression of vitamin D receptor in bone of immobilized immature rat and explore the relation between disuse osteoporosis(DOP)and the expression of vitamin D receptor(VDR)in bone.Methods Rats aging 4 weeks were immobilized by limb-tail fixation for 2 and 4 weeks respectively,and then the wet weight,gray weight,bone mineral density of femurs and tibiae were measured,the histopathological and histomorphological parameters also were detected,which were used to evaluate the effect of immobilization on bone content and bone structure of femurs and tibiae in growing rats.The VDR expression of femurs and tibiae in the growing rats was evaluated by immunohistochemistry and image analysis.Results The wet weight,gray weight,and bone mineral density of immobilized limbs in the growing rats were all lower than those in age-matched control groups(P＜0.05).There were rare,small and broken bone trabeculae found in the histological films of proximal tibial metaphysis of immobilized limbs,with decreased trabecular area and perimeter(P＜0.05),comparing with the age-macthed control groups.Over-expression of VDR in cellular plasma and nuclear membranes of osteoblasts was also found in the proximal metaphysis of right hind limb in immobilized growing rats.Conclusions It is a definite method to establish animal model of DOP in young rats by leg-tail fixation;over-expression of Vitamin D receptors in growing bone of immobilized rat limbs means that the disorder of vittamin D endocrine system may be related to pathogenesis of DOP.