中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2010年
4期
300-303
,共4页
更年期抑郁症%5-羟色胺%色氦酸羟化酶2
更年期抑鬱癥%5-羥色胺%色氦痠羥化酶2
경년기억욱증%5-간색알%색양산간화매2
Pefimenopausal depression%5-hydroxytryptamine%Tryptophan hydroxylase 2
目的 制备更年期抑郁症大鼠模型,观察补益肝肾中药对模型大鼠海马5-羟色胺(5-HT)含量及其5-HT合成限速酶-色氨酸羟化酶2(TPH2)表达的影响,探讨补益肝肾中药防治更年期抑郁症的机理.方法 采用雌性SD大鼠,在卵巢切除术的基础上,联合孤养和21d慢性不可预见性的温和刺激(CUMS)制备更年期抑郁症大鼠模型.以高效液相色谱.电化学检测法(HPLC-ECD)测定海马5-HT含量、RT-PCR技术检测中缝核TPH2 mRNA表达以及荧光免疫组化检测中缝核TPH2蛋白表达.结果 中药组海马5-HT含量高于模型组,但差异无显著性[(2543.06±859.59)ng/g组织,(1845.81±233.55)ng/g组织,F=9.617,P＞0.05].RT-PCR示中药组中缝核TPH2 mRNA相对表达量明显高于模型组[(1.282±0.158),(0.985±0.120),F=3.552,P＜0.05].荧光免疫组化示中药组TPH2阳性细胞数明显多于模型组[(152.74±68.52)个,(74.12±38.01)个,F=7.939,P＜0.01].结论 补益肝肾中药具有防治更年期抑郁症的作用,提高大鼠中缝核TPH2的表达,促进5-HT的合成,使海马5-HT含量升高,可能是补益肝肾中药防治更年期抑郁症的作用途径之一.
目的 製備更年期抑鬱癥大鼠模型,觀察補益肝腎中藥對模型大鼠海馬5-羥色胺(5-HT)含量及其5-HT閤成限速酶-色氨痠羥化酶2(TPH2)錶達的影響,探討補益肝腎中藥防治更年期抑鬱癥的機理.方法 採用雌性SD大鼠,在卵巢切除術的基礎上,聯閤孤養和21d慢性不可預見性的溫和刺激(CUMS)製備更年期抑鬱癥大鼠模型.以高效液相色譜.電化學檢測法(HPLC-ECD)測定海馬5-HT含量、RT-PCR技術檢測中縫覈TPH2 mRNA錶達以及熒光免疫組化檢測中縫覈TPH2蛋白錶達.結果 中藥組海馬5-HT含量高于模型組,但差異無顯著性[(2543.06±859.59)ng/g組織,(1845.81±233.55)ng/g組織,F=9.617,P＞0.05].RT-PCR示中藥組中縫覈TPH2 mRNA相對錶達量明顯高于模型組[(1.282±0.158),(0.985±0.120),F=3.552,P＜0.05].熒光免疫組化示中藥組TPH2暘性細胞數明顯多于模型組[(152.74±68.52)箇,(74.12±38.01)箇,F=7.939,P＜0.01].結論 補益肝腎中藥具有防治更年期抑鬱癥的作用,提高大鼠中縫覈TPH2的錶達,促進5-HT的閤成,使海馬5-HT含量升高,可能是補益肝腎中藥防治更年期抑鬱癥的作用途徑之一.
목적 제비경년기억욱증대서모형,관찰보익간신중약대모형대서해마5-간색알(5-HT)함량급기5-HT합성한속매-색안산간화매2(TPH2)표체적영향,탐토보익간신중약방치경년기억욱증적궤리.방법 채용자성SD대서,재란소절제술적기출상,연합고양화21d만성불가예견성적온화자격(CUMS)제비경년기억욱증대서모형.이고효액상색보.전화학검측법(HPLC-ECD)측정해마5-HT함량、RT-PCR기술검측중봉핵TPH2 mRNA표체이급형광면역조화검측중봉핵TPH2단백표체.결과 중약조해마5-HT함량고우모형조,단차이무현저성[(2543.06±859.59)ng/g조직,(1845.81±233.55)ng/g조직,F=9.617,P＞0.05].RT-PCR시중약조중봉핵TPH2 mRNA상대표체량명현고우모형조[(1.282±0.158),(0.985±0.120),F=3.552,P＜0.05].형광면역조화시중약조TPH2양성세포수명현다우모형조[(152.74±68.52)개,(74.12±38.01)개,F=7.939,P＜0.01].결론 보익간신중약구유방치경년기억욱증적작용,제고대서중봉핵TPH2적표체,촉진5-HT적합성,사해마5-HT함량승고,가능시보익간신중약방치경년기억욱증적작용도경지일.
Objective To observe the effect of Chinese medicine of replenishing the liver and kidney on the concentration of 5-hydroxytryptamine(5-HT)in hippocampus and the expression of tryptophan hydroxylase 2 (TPH2)in median raphe nuclei in perimenopausal depression model rats and to explore its mechanism.Methods The animal model was established by resecting the ovaries of female rats,then giving isolation-housing in combination with 21 days of chronic unexpected mild stress(CUMS).High performance liquid chromatogram-electrochemical detection(HPLC-ECD)were employed to measure 5-HT in hippocampus,the hemi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)and fluorescence immunohistochemistry were employed to detect the relative expression of TPH2 mRNA and number of TPH2 immunoreactive neurons in median raphe nuclei.Results The concentration of 5-HT in hippocampus was increased in rats of the medicine group compared with that in the model group((2543.06±859.59)ng/g tissue,(1845.81±233.55)ng/g tissue,F=9.617,P＞0.05).RT-PCR showed that the relative expression of TPH2 mRNA in medicine group was much higher than that in model group ((1.282±0.158),(0.985±0.120),F=3.552,P＜0.05).Immunofluorescence showed that the number of TPH2 immunoreaetive neurons in raphe nuclei had statistical significance between medicine group and model group ((152.74±68.52),(74.12±38.01),F=7.939,P＜0.01).Conclusion Chinese medicine replenishing liver and kidney have effects of improving perimenopausal depression,increasing the expression of TPH2 in median raphe nuclei,which leads to enhance the synthesis of 5-HT in hippocampus might be one of its mechanisms.
