CAJ | 학술논문

目的探讨介导血红素氧化酶-1( heme oxygenase,HO-1)的腺病毒在治疗急性放射性皮肤损伤中的作用.方法选取33只雄性SD大鼠,电脑数字表法随机分为3组,磷酸盐缓冲液(PBS)对照组、介导绿色荧光蛋白基因的腺病毒(Ad-EGFP)组及介导HO-1基因的腺病毒(Ad-HO-1)组,每组11只.每组取3只大鼠,用于检测腺病毒目的基因的表达.用直线加速器产生的40 Gy负电子线照射各组余下大鼠臀部皮肤.照射后立即分别皮下注射PBS、Ad-EGFP或Ad-HO-1.采用半定量方法对皮肤损伤评分,每周评定2次,观察7周.结果注射Ad-HO-1的皮肤真皮层内见HO-1表达强阳性.照射14 d后,Ad-HO-1组皮肤损伤明显低于PBS对照组和Ad-EGFP组(q=0.000 ～0.030,P＜0.05).结论 HO-1能显著减轻急性放射性皮肤损伤,表明Ad-HO-1可以用于治疗放射性皮肤损伤.
목적 탐토개도혈홍소양화매-1( heme oxygenase,HO-1)적선병독재치료급성방사성피부손상중적작용.방법 선취33지웅성SD대서,전뇌수자표법수궤분위3조,린산염완충액(PBS)대조조、개도록색형광단백기인적선병독(Ad-EGFP)조급개도HO-1기인적선병독(Ad-HO-1)조,매조11지.매조취3지대서,용우검측선병독목적기인적표체.용직선가속기산생적40 Gy부전자선조사각조여하대서둔부피부.조사후립즉분별피하주사PBS、Ad-EGFP혹Ad-HO-1.채용반정량방법대피부손상평분,매주평정2차,관찰7주.결과 주사Ad-HO-1적피부진피층내견HO-1표체강양성.조사14 d후,Ad-HO-1조피부손상명현저우PBS대조조화Ad-EGFP조(q=0.000 ～0.030,P＜0.05).결론 HO-1능현저감경급성방사성피부손상,표명Ad-HO-1가이용우치료방사성피부손상.
Objective To investigate the effect of heme oxygenase-1 ( HO-1 ) on the acute radiation-induced skin injury by gene transfer.Methods Thirty-three male SD rats were randomly divided into three groups as PBS-injected group,Ad-EGFP-injeeted group and Ad-HO-1-injected group ( n =11 ).In each group,three rats were used for determining the expression of target gene and the other rats were irradiated on the buttock skin with 40 Gy electron beam generated by a linear accelerator.Immediately after irradiation,rats were administered with a subcutaneous injection of PBS,Ad-EGFP or Ad-HO-1,respectively.Subsequently,the skin reactions were measured twice a week using the semi-quantitative skin injury scale.Results The strong positive expression of HO-1 was observed in subcutaneous dermal tissue after injection of Ad-HO-1.Compared to the PBS-injected group or the Ad-EGFP-injected group,a significant mitigation of skin injury was observed in Ad-HO-1-injected mice 14 d after irradiation (q =0.000-0.030,P ＜ 0.05 ).Conclusions HO-1 could significantly mitigate radiation-induced acute skin injury and Ad-HO-1 could be used to treat radiation-induced skin injury.