中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2010年
19期
2605-2607
,共3页
动脉粥样硬化%通心络%内皮功能%抗原,CD40%CD40配体
動脈粥樣硬化%通心絡%內皮功能%抗原,CD40%CD40配體
동맥죽양경화%통심락%내피공능%항원,CD40%CD40배체
Artherosclerosis%Tongxinluo%Endodermis function%Antigens,CD40%CD40 ligand
目的 观察通心络对动脉粥样硬化家免模型的预防治疗作用,并探讨其对内皮功能、CD40及CD40L mRNA表达的影响.方法 选取60只雄性健康家兔,随机分为对照组、模型组、通心络组三组,每组20只,后两组家兔分别通过高脂饮食制备动脉粥样硬化模型.给药处理前及12周后分别测定以下指标:血浆总胆固醇(TC)、甘油三酯(TG)和高密度酯蛋白胆固醇(HDL-C)的浓度;主动脉内膜/中膜厚度比值及粥样斑块面积;测定血清内皮素(ET)水平和血清一氧化氮(NO)水平;运用定量逆转尿PCR技术(RT-PCR)测定外周血单核细胞中CD40及CD40L mRNA的表达水平.结果 模型组和通心络组家免的TC水平均显著高于对照组(P<0.01),通心络组又低于模型组(P<0.05);通心络组血清ET水平和ET/NO显著降低(P<0.05,P<0.01),血清NO水平显著升高(P<0.01);通心络组中,家兔的主动脉内膜/中膜厚度比值及斑块面积占血管总面积百分率均明显低于模型组[(0.56±0.07)与(1.16±0.08),P<0.01;(36.88±2.38)%与(76.58±2.86)%,P<0.01];与模型组(0.798±0.115)、(0.592±0.132)比较,通心络组家兔外周血单-核细胞中的CD40及CD40L mRNA表达量明显降低[(0.686±0.132)、(0.498±0.108)](P<0.01).结论 通心络可抑制动脉粥样硬化斑块的形成,其机制可能下调CD40及CD40L表达及有效地改善内皮功能有关.
目的 觀察通心絡對動脈粥樣硬化傢免模型的預防治療作用,併探討其對內皮功能、CD40及CD40L mRNA錶達的影響.方法 選取60隻雄性健康傢兔,隨機分為對照組、模型組、通心絡組三組,每組20隻,後兩組傢兔分彆通過高脂飲食製備動脈粥樣硬化模型.給藥處理前及12週後分彆測定以下指標:血漿總膽固醇(TC)、甘油三酯(TG)和高密度酯蛋白膽固醇(HDL-C)的濃度;主動脈內膜/中膜厚度比值及粥樣斑塊麵積;測定血清內皮素(ET)水平和血清一氧化氮(NO)水平;運用定量逆轉尿PCR技術(RT-PCR)測定外週血單覈細胞中CD40及CD40L mRNA的錶達水平.結果 模型組和通心絡組傢免的TC水平均顯著高于對照組(P<0.01),通心絡組又低于模型組(P<0.05);通心絡組血清ET水平和ET/NO顯著降低(P<0.05,P<0.01),血清NO水平顯著升高(P<0.01);通心絡組中,傢兔的主動脈內膜/中膜厚度比值及斑塊麵積佔血管總麵積百分率均明顯低于模型組[(0.56±0.07)與(1.16±0.08),P<0.01;(36.88±2.38)%與(76.58±2.86)%,P<0.01];與模型組(0.798±0.115)、(0.592±0.132)比較,通心絡組傢兔外週血單-覈細胞中的CD40及CD40L mRNA錶達量明顯降低[(0.686±0.132)、(0.498±0.108)](P<0.01).結論 通心絡可抑製動脈粥樣硬化斑塊的形成,其機製可能下調CD40及CD40L錶達及有效地改善內皮功能有關.
목적 관찰통심락대동맥죽양경화가면모형적예방치료작용,병탐토기대내피공능、CD40급CD40L mRNA표체적영향.방법 선취60지웅성건강가토,수궤분위대조조、모형조、통심락조삼조,매조20지,후량조가토분별통과고지음식제비동맥죽양경화모형.급약처리전급12주후분별측정이하지표:혈장총담고순(TC)、감유삼지(TG)화고밀도지단백담고순(HDL-C)적농도;주동맥내막/중막후도비치급죽양반괴면적;측정혈청내피소(ET)수평화혈청일양화담(NO)수평;운용정량역전뇨PCR기술(RT-PCR)측정외주혈단핵세포중CD40급CD40L mRNA적표체수평.결과 모형조화통심락조가면적TC수평균현저고우대조조(P<0.01),통심락조우저우모형조(P<0.05);통심락조혈청ET수평화ET/NO현저강저(P<0.05,P<0.01),혈청NO수평현저승고(P<0.01);통심락조중,가토적주동맥내막/중막후도비치급반괴면적점혈관총면적백분솔균명현저우모형조[(0.56±0.07)여(1.16±0.08),P<0.01;(36.88±2.38)%여(76.58±2.86)%,P<0.01];여모형조(0.798±0.115)、(0.592±0.132)비교,통심락조가토외주혈단-핵세포중적CD40급CD40L mRNA표체량명현강저[(0.686±0.132)、(0.498±0.108)](P<0.01).결론 통심락가억제동맥죽양경화반괴적형성,기궤제가능하조CD40급CD40L표체급유효지개선내피공능유관.
Objective To investigate the therapeutic effects of Tongxinluo and its influence on the expression of CD40 and CD40L in artherosclerosis rabbits. Methods 60 male rabbits were divided into three groups randomly,20 of each group: normal control group, model group and Tongxinluo group. The rabbits in model group and Tongxinluogroup were used to prepare the atherosclerosis model induced by high-cholesterol diet. The levels of ET-1, NOwere measurde. Before and 12 weeks after durg treatment,serum total cholesterol ,plaque areas and ratios of intima/media thickness were detected. The expression of CD40 and CD40L mRNA were determinated by quantitive RT-PCR. Results Serum total cholesterol level of Tongxinluo group was decreased compared with model group ( P < 0. 05 ), and they were both much higher than those of normal control group ( P < 0. 01 ); as compared with the model group, aortic plaque areas and ratios of intima/media thickness in Tongxinluo group reduced significantly [ (0.56 ± 0. 07) vs ( 1.16±0.08),P<0.01;(36.88±2.38)% vs (76.58 ±2.86) %,P <0. 0l] ;The expression of CD40 and CD40L mRNA also decreased in Tongxinluo group with statistic significance[ (0.798 ± 0.115 )、 (0. 592 ± 0. 132) vs (0.686±0. 132) 、(0.498 ±0. 108) ,P <0. 01 ) ]. Conclusion Tongxinluo had the ability to anti-artherosclerosis and its possible mechanism was down-regulate the expression of CD40 and CD40L.
